| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,050,271 | A→G | 11.6% | L28L (CTT→CTC) | glnE ← | fused deadenylyltransferase and adenylyltransferase for glutamine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,050,271 | 0 | A | G | 11.6% | 182.6 / 12.0 | 69 | L28L (CTT→CTC) | glnE | fused deadenylyltransferase and adenylyltransferase for glutamine synthetase |
| Reads supporting (aligned to +/- strand): ref base A (32/29); new base G (3/5); total (35/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACG > minE/2050202‑2050335 | ctctGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTaa < 1:772090/71‑1 (MQ=255)ctctGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTaa < 1:3391693/71‑1 (MQ=255) tctGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAg > 1:100386/1‑71 (MQ=255) tctGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAg > 1:619103/1‑71 (MQ=255) gATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGt < 1:576242/68‑1 (MQ=255) gATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGa > 1:3764625/1‑70 (MQ=255) gATGCGCAATCACGCTGTCATGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTGAGt < 1:1419301/68‑1 (MQ=255) tGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTc < 1:3033642/71‑1 (MQ=255) cgcAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTcc < 1:283945/70‑1 (MQ=255) cgcAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCt < 1:2695980/71‑1 (MQ=255) cgcAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCt < 1:1485093/71‑1 (MQ=255) gcAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTc > 1:1457580/1‑71 (MQ=255) cAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTc < 1:2756460/70‑1 (MQ=255) cAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCg < 1:1348576/71‑1 (MQ=255) aaTCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCgg > 1:229823/1‑71 (MQ=255) tCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGc > 1:302604/1‑70 (MQ=255) acACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGc > 1:3591640/2‑70 (MQ=255) cGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTaaa < 1:771804/71‑1 (MQ=255) gCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAg > 1:195551/1‑71 (MQ=255) tCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGctc > 1:2399702/1‑71 (MQ=255) gCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTa < 1:752157/44‑1 (MQ=255) gCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTGAGTGATTCCTCGGCTAAAGGctc > 1:3280140/1‑67 (MQ=255) gCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTg > 1:3167678/1‑48 (MQ=255) gCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTg > 1:3263152/1‑48 (MQ=255) gCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTgg > 1:1391159/1‑70 (MQ=255) gCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTgg > 1:2249431/1‑70 (MQ=255) cacaAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCt < 1:2820736/53‑1 (MQ=255) cacaAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGc < 1:928263/70‑1 (MQ=255) caAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGc < 1:609910/71‑1 (MQ=255) aaaaTCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGcc > 1:2545204/1‑71 (MQ=255) aaaaTCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGcc > 1:699289/1‑71 (MQ=255) aaaaTCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGc > 1:704041/1‑70 (MQ=255) aaaTCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTGAGTGATTc < 1:3638997/47‑1 (MQ=255) aaaTCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGcc < 1:2038484/70‑1 (MQ=255) aaTCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCg > 1:1497799/1‑70 (MQ=255) aaTCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGc < 1:3293650/71‑1 (MQ=255) aaTCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGc > 1:1746218/1‑71 (MQ=255) aaTCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGc < 1:1698481/71‑1 (MQ=255) aTCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCt < 1:141072/71‑1 (MQ=255) tCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTc < 1:1958948/44‑1 (MQ=255) tCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTc < 1:2597044/44‑1 (MQ=255) cACTAAAAGTAAGTACTGACTTAGCCTGTGCGCTAAGTGATTCCTCGGCTaaa > 1:1068793/1‑53 (MQ=255) cTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTcaac < 1:344358/71‑1 (MQ=255) tAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGt < 1:292202/35‑1 (MQ=255) aaaGTAAGTACTGACTTCGCCTGTGCGCTGAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCaacaac > 1:2762493/1‑71 (MQ=255) aaaGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCt > 1:667247/1‑40 (MQ=255) aaaGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGc > 1:2765142/1‑60 (MQ=255) aaaGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGc > 1:1226133/1‑60 (MQ=255) aaaGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTcaacaa < 1:2401014/70‑1 (MQ=255) aaaGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTcaaca < 1:2449733/69‑1 (MQ=255) aagtaagtACTGACTTCGCCTGTGCGCTGAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACg < 1:64636/71‑1 (MQ=255) aagtaagtACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACg < 1:2984599/71‑1 (MQ=255) aagtaCTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAg > 1:1680665/1‑54 (MQ=255) aagtaCTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTc < 1:124719/70‑1 (MQ=255) agtaCTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACg > 1:2393610/1‑66 (MQ=255) agtaCTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCt > 1:2493331/1‑70 (MQ=255) taCTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGc > 1:811226/1‑70 (MQ=255) taCTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGc > 1:3859748/1‑70 (MQ=255) taCTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGc > 1:2691137/1‑70 (MQ=255) cTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGcc < 1:815599/51‑1 (MQ=255) tGACTTCGCCTGTGCGCTAAGTGATTCCTCTGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCt < 1:1314841/65‑1 (MQ=255) aCTTCGCCTGTGCGCTGAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTAc < 1:2502602/71‑1 (MQ=255) aCTTCGCCTGTGCGCTGAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTAc < 1:2497974/71‑1 (MQ=255) aCTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTa < 1:13359/70‑1 (MQ=255) ttCGCCTGTGCGCTGAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTActg > 1:382593/1‑71 (MQ=255) ttCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTActg > 1:449724/1‑71 (MQ=255) cGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTActgct > 1:1030492/1‑71 (MQ=255) cGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTActgct > 1:1267234/1‑71 (MQ=255) cGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTActg < 1:1187611/69‑1 (MQ=255) gCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTActgctg > 1:3702292/1‑71 (MQ=255) cTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAg < 1:1767293/61‑1 (MQ=255) tgCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACg < 1:929777/71‑1 (MQ=255) | CTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACG > minE/2050202‑2050335 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |