| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,057,586 | C→T | 18.3% | D137D (GAC→GAT) | ttdA → | L‑tartrate dehydratase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,057,586 | 0 | C | T | 18.3% | 158.4 / 27.0 | 71 | D137D (GAC→GAT) | ttdA | L‑tartrate dehydratase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (30/28); new base T (7/6); total (37/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
CGGTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTGCACGCTACCTGG > minE/2057516‑2057654 | cGGTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGAc < 1:443204/71‑1 (MQ=255) gTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGAcat < 1:151021/71‑1 (MQ=255) gTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGAcat < 1:3377892/71‑1 (MQ=255) tAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGAcatc < 1:16303/71‑1 (MQ=255) aGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGAcatca > 1:1532465/1‑71 (MQ=255) aGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGAcatca > 1:3371715/1‑71 (MQ=255) aaTTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATcc > 1:2242093/1‑71 (MQ=255) aTTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATccc > 1:3163398/1‑71 (MQ=255) gAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGGtatcat > 1:2772738/1‑59 (MQ=255) gAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGATATCATCCCCGACAATGa > 1:2078265/1‑71 (MQ=255) gAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGATATCATCCCCGACAATGa > 1:3877462/1‑71 (MQ=255) gAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAAt < 1:1006417/69‑1 (MQ=255) aaGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGAc < 1:1753181/71‑1 (MQ=255) aGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGAc > 1:875816/1‑70 (MQ=255) gTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGa > 1:2685767/1‑71 (MQ=255) gTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGa > 1:2681307/1‑71 (MQ=255) gTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGa > 1:1589368/1‑71 (MQ=255) aaCACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGc > 1:3484009/1‑71 (MQ=255) aaCACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATg > 1:3770979/1‑70 (MQ=255) aaCACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGc < 1:1526096/71‑1 (MQ=255) aaCACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGc > 1:1395241/1‑71 (MQ=255) aaCACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGc < 1:1535222/71‑1 (MQ=255) cacCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCgg < 1:3217085/71‑1 (MQ=255) acCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCgg > 1:2754577/1‑70 (MQ=255) aaaaaTACCGGTAGCGGCGTACCGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCGGAAATCGa < 1:3837916/71‑1 (MQ=255) aaaaaTACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATcccc < 1:1491241/48‑1 (MQ=255) aaaaTACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGaa < 1:587107/71‑1 (MQ=255) aaaaTACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGaa < 1:3353877/71‑1 (MQ=255) aaaTACCGGTAGCGGCGTACCGTGGGTCACCTGGGACatcatc > 1:2559559/1‑43 (MQ=255) aaaTACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCg > 1:3029287/1‑68 (MQ=255) aaTACCGGTAGCGGCTTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGt < 1:3443623/71‑1 (MQ=255) aaTACCGGTAGCGGCGTACCGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCGGAATTCGAAGt < 1:1868175/71‑1 (MQ=255) aaTACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGt > 1:2769251/1‑71 (MQ=255) aTACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGtt > 1:841813/1‑71 (MQ=255) ggTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACa > 1:2489920/1‑70 (MQ=255) gTAGCGGCGTACCGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATg > 1:2364124/1‑71 (MQ=255) gTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACaa > 1:1666257/1‑43 (MQ=255) gTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGa > 1:3477514/1‑46 (MQ=255) gTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGa > 1:2752560/1‑46 (MQ=255) gTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACg < 1:1995158/48‑1 (MQ=255) gTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACg < 1:3485036/48‑1 (MQ=255) gTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATg > 1:1655358/1‑71 (MQ=255) tAGCGGCGTACCGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATgg < 1:1003747/71‑1 (MQ=255) tAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATgg < 1:33795/71‑1 (MQ=255) gcggcgTACCGTGGGTCACCTGGGACATCATCCCCGa > 1:2412696/1‑37 (MQ=255) gcggcgTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCa < 1:1685163/71‑1 (MQ=255) gcgTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTAc < 1:1606771/62‑1 (MQ=255) gTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGa < 1:3157787/53‑1 (MQ=255) gTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGa < 1:535677/53‑1 (MQ=255) gTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGa < 1:1581983/53‑1 (MQ=255) gTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAggcgg > 1:1800432/1‑71 (MQ=255) gTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAggcgg < 1:3461301/71‑1 (MQ=255) ccGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAggcggcgg > 1:288371/1‑71 (MQ=255) gTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCt > 1:708804/1‑71 (MQ=255) gTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACa > 1:537235/1‑56 (MQ=255) tGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTg < 1:910924/71‑1 (MQ=255) ggTCACCTGGGACATCATCCCCGACAATGACGATGCGGa < 1:2114545/39‑1 (MQ=255) ggTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTGc < 1:1096458/70‑1 (MQ=255) ggTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTGCa < 1:1382298/71‑1 (MQ=255) tCACCTGGGATATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTGCACg < 1:454669/71‑1 (MQ=255) tCACCTGGGACATCATCCCCGACAATGACGATGCGGa < 1:1456607/37‑1 (MQ=255) tCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCg < 1:637029/42‑1 (MQ=255) tCACCTGGGACATCATCACCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTGCACg > 1:1274148/1‑71 (MQ=255) aCCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTGCACGc > 1:2302100/1‑70 (MQ=255) cTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAg > 1:1193979/1‑41 (MQ=255) cTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTGCa > 1:2286716/1‑65 (MQ=255) cTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTGCACGCTAc < 1:1227953/71‑1 (MQ=255) gggACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTa > 1:2569956/1‑43 (MQ=255) gggACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACa > 1:1768215/1‑45 (MQ=255) gggACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACa > 1:1728416/1‑45 (MQ=255) gggACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACa > 1:1365444/1‑45 (MQ=255) gggACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACAt < 1:1306797/46‑1 (MQ=255) gggACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACAt < 1:3549069/46‑1 (MQ=255) gggACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAggcggc < 1:1016804/56‑1 (MQ=255) gggACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTg > 1:1907757/1‑61 (MQ=255) ggATATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTGCACGCTAcc < 1:683603/69‑1 (MQ=255) ggATATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTGCACGCTACCTg > 1:1230654/1‑71 (MQ=255) gACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTGCACGCTACCTgg < 1:2165486/71‑1 (MQ=255) | CGGTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTGCACGCTACCTGG > minE/2057516‑2057654 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |