| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,068,671 | C→T | 12.0% | A373A (GCG→GCA) | aer ← | fused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,068,671 | 0 | C | T | 12.0% | 131.3 / 8.3 | 50 | A373A (GCG→GCA) | aer | fused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component |
| Reads supporting (aligned to +/- strand): ref base C (25/19); new base T (2/4); total (27/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
AAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATG > minE/2068603‑2068740 | aaaTGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAccgc > 1:3403829/1‑71 (MQ=255)aaaTGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAccgc > 1:231308/1‑71 (MQ=255) cGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt < 1:3730117/71‑1 (MQ=255) cGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt < 1:3435111/71‑1 (MQ=255) cACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCa < 1:2029644/71‑1 (MQ=255) cACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCa < 1:2034237/71‑1 (MQ=255) cTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAgg > 1:3333029/1‑71 (MQ=255) cTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAgg > 1:2427210/1‑71 (MQ=255) cTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAgg > 1:2422504/1‑71 (MQ=255) ttCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAggg > 1:3030166/1‑71 (MQ=255) tcaccTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGc > 1:2678180/4‑71 (MQ=255) tCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGc > 1:522385/1‑71 (MQ=255) caccTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGc < 1:203244/68‑1 (MQ=255) ccTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAg < 1:712940/71‑1 (MQ=255) tGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACcgccgc > 1:549988/1‑57 (MQ=255) tGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCa > 1:437268/1‑58 (MQ=255) gCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa > 1:3066835/1‑70 (MQ=255) gCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa > 1:3533332/1‑70 (MQ=255) ccaccaCTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa < 1:1781001/69‑1 (MQ=255) ccaccaCTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAat < 1:436552/70‑1 (MQ=255) accacTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa < 1:3054758/67‑1 (MQ=255) cTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTc > 1:564839/1‑71 (MQ=255) tGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCAt < 1:3442404/50‑1 (MQ=255) tGCAAAACCTTTGCCCTGTTCGCCGGCACCCGCCGCTTCCACCGCCGCAt < 1:797656/50‑1 (MQ=255) cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCt > 1:591776/1‑69 (MQ=255) cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGa < 1:343315/71‑1 (MQ=255) aaaaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGaa < 1:3173156/71‑1 (MQ=255) aaaaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGa > 1:2974443/1‑70 (MQ=255) aaaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCa > 1:1016410/1‑45 (MQ=255) aaaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCa > 1:1879894/1‑45 (MQ=255) aCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc > 1:2651862/1‑70 (MQ=255) accttGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGc < 1:2492293/68‑1 (MQ=255) cccTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCa > 1:1596129/1‑40 (MQ=255) ccTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCg < 1:2028251/71‑1 (MQ=255) ccTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCg < 1:1350658/71‑1 (MQ=255) cTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAattc > 1:3663648/1‑66 (MQ=255) ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCg < 1:2259955/67‑1 (MQ=255) ccgCACGCGCAGCCTCAACCGCCGCGTTCAAAGCCAGAATATTGGTCTGGAAGGCAATGCCGTCAAt < 1:2790610/65‑1 (MQ=255) cGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGc > 1:1329879/1‑71 (MQ=255) gCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGa < 1:3277613/71‑1 (MQ=255) gCACGCGCAGCCTCAACCGCCGCGTTCAAAGCCAGAATATTGGTCTGGAAGGCAat > 1:2499203/1‑56 (MQ=16) cACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCg > 1:3028283/1‑69 (MQ=255) cACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCg > 1:192375/1‑69 (MQ=255) cgcgCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGt < 1:868334/71‑1 (MQ=255) gccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAAt > 1:3011232/1‑71 (MQ=255) gccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAAt < 1:2754838/71‑1 (MQ=255) cTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTa < 1:2898855/53‑1 (MQ=255) cTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGc < 1:899517/59‑1 (MQ=255) cTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGt > 1:3104982/1‑70 (MQ=255) tCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGt > 1:1540306/1‑68 (MQ=255) cACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCaa > 1:3825574/1‑71 (MQ=255) cACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCaa > 1:814492/1‑71 (MQ=255) aCCGCCGCATTCAGGGCCAGAATATAGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAAt > 1:1433693/1‑71 (MQ=255) ccgccgCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAAt < 1:2813940/70‑1 (MQ=255) ccgccgCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATg > 1:3786816/1‑71 (MQ=255) | AAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATG > minE/2068603‑2068740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |