| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,072,881 | C→A | 16.3% | P235T (CCG→ACG) | ebgR → | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,072,881 | 0 | C | A | 16.3% | 169.7 / 14.3 | 61 | P235T (CCG→ACG) | ebgR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (28/23); new base A (6/4); total (34/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01 | |||||||||||
GCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCC > minE/2072814‑2072950 | gcgcGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCg < 1:1897882/70‑1 (MQ=255) cgcgGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATcc > 1:1254708/1‑68 (MQ=255) cgcgGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGaa < 1:1405886/71‑1 (MQ=255) cggcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAgg < 1:37087/71‑1 (MQ=255) tttttCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACtgt > 1:2871061/1‑71 (MQ=255) ttttCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACtgtt > 1:1300068/1‑71 (MQ=255) ttCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACtgtttg > 1:1442714/1‑71 (MQ=255) tCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACtgtttg < 1:3746635/70‑1 (MQ=255) ccAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACtgtttgtt > 1:1074498/1‑71 (MQ=255) ccAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACtgtttgt < 1:1719295/70‑1 (MQ=255) ccAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACtgtttg < 1:3159979/69‑1 (MQ=255) gTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCt > 1:2865978/1‑71 (MQ=255) ttCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATc < 1:3605870/50‑1 (MQ=255) ttCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCtt > 1:1758208/1‑71 (MQ=255) tcgGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGa > 1:673276/1‑71 (MQ=255) ggTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTc < 1:2872583/71‑1 (MQ=255) ttATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGa > 1:1974280/1‑44 (MQ=255) ttATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGa > 1:3357628/1‑44 (MQ=255) ttATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCa < 1:2427224/71‑1 (MQ=255) tATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTc > 1:876130/1‑62 (MQ=255) tGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTg > 1:3130993/1‑71 (MQ=255) gAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCt < 1:703837/57‑1 (MQ=255) aCTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACtgtttgt < 1:3252624/51‑1 (MQ=255) aCTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTa < 1:1700328/71‑1 (MQ=255) aCTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTa < 1:3648844/71‑1 (MQ=255) tGGCAAAACAAATGCTGGCGCGGGAAGACTATCCg < 1:99526/35‑1 (MQ=255) tGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGaa > 1:1453035/1‑37 (MQ=255) tGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGaa > 1:2738552/1‑37 (MQ=255) tGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCa > 1:3391260/1‑63 (MQ=255) tGGAAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATc > 1:3465894/1‑71 (MQ=255) gCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACtgtttgtt > 1:1085380/1‑48 (MQ=255) aaaaCAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGc > 1:1298437/1‑70 (MQ=255) aaCAAATGCTGGCGCGGGAAGACTATCCGAAGGCACtgt > 1:1200843/1‑39 (MQ=255) aCAAATGCTGGCGCGGGAAGACTATCCGAAGGCACtgtttgt > 1:1629416/1‑42 (MQ=255) aCAAATGCTGGCGCGGGAAGACTATACGAAGGCACtgtttgtt > 1:3471521/1‑43 (MQ=255) aCAAATGCTGGCGCGGGAAGACTATACGAAGGCACtgtttgtt > 1:3216080/1‑43 (MQ=255) aCAAATGCTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTAc < 1:1622626/71‑1 (MQ=255) cAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGc > 1:2059113/1‑66 (MQ=255) aaaTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCa < 1:834378/54‑1 (MQ=255) aaTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGc < 1:2419294/71‑1 (MQ=255) aTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCg > 1:3952681/1‑71 (MQ=255) aTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCg > 1:3477610/1‑71 (MQ=255) aTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCg > 1:206600/1‑71 (MQ=255) cTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCggg > 1:2611669/1‑70 (MQ=255) gcgcGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCa < 1:3232696/69‑1 (MQ=255) gcgcGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAAt < 1:185058/71‑1 (MQ=255) gcgcGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAAt < 1:3453220/71‑1 (MQ=255) gcgcGGGAAGACTATACGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCaa < 1:3284072/70‑1 (MQ=255) cgcgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAAtt > 1:973466/1‑71 (MQ=255) gcgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGTCGTACTGCGGGCAAtt < 1:88217/70‑1 (MQ=255) gcgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAAtt > 1:1201872/1‑70 (MQ=255) gcgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTc < 1:2240311/71‑1 (MQ=255) cgGGAAGACTATACGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCa < 1:565971/71‑1 (MQ=255) gggAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCAt > 1:1210588/1‑71 (MQ=255) ggAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATg < 1:1011626/71‑1 (MQ=255) aGACTATACGAAGGCACTGTTTGTTGCTTCCGATTCCATTGc > 1:1559072/1‑42 (MQ=255) aGACTATACGAAGGCACTGTTTGTTGCTTCCGATTCCATTGc > 1:1764436/1‑42 (MQ=255) aCTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCg < 1:1108627/48‑1 (MQ=255) tATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGa > 1:2818459/1‑69 (MQ=255) tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTAc > 1:2166367/1‑47 (MQ=255) tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAgg < 1:3265149/69‑1 (MQ=255) tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAgg < 1:2245572/69‑1 (MQ=255) tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGcc > 1:3563843/1‑71 (MQ=255) | GCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCC > minE/2072814‑2072950 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |