| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,087,666 | C→T | 50.0% | T54I (ACC→ATC) | yhaH → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,087,666 | 0 | C | T | 50.0% | ‑4.1 / 81.9 | 58 | T54I (ACC→ATC) | yhaH | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (13/16); new base T (15/14); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTG > minE/2087599‑2087730 | atcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCt < 1:1160262/71‑1 (MQ=255)atcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCt < 1:1718860/71‑1 (MQ=255) cTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTga > 1:2107860/1‑71 (MQ=255) aCGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTgacga > 1:1870671/1‑70 (MQ=255) cGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACa < 1:3506990/71‑1 (MQ=255) gTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGTGAAGGCACCCTGACGACaa < 1:2933982/71‑1 (MQ=255) tCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATc > 1:1884559/1‑71 (MQ=255) cGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATc < 1:2078660/70‑1 (MQ=255) tGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTAc < 1:3219448/71‑1 (MQ=255) gCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACg > 1:866703/1‑71 (MQ=255) gggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCAtcc > 1:1140223/1‑52 (MQ=255) gggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTAt > 1:1847813/1‑71 (MQ=255) tgctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATc < 1:2522821/59‑1 (MQ=255) tgctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTg > 1:1375785/1‑71 (MQ=255) tgctgGATAAAATGTTAGGCTGGCAACGCGCTGGGGGCGAAGGCACCCTGACGACAATCTACGGTATTc < 1:1294701/69‑1 (MQ=255) gctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTa < 1:47548/60‑1 (MQ=255) tgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGgtg > 1:1471453/1‑71 (MQ=255) tgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTga > 1:2448509/1‑48 (MQ=255) ggATAAGATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGgtgt > 1:3818/1‑71 (MQ=255) gATAAAATGTTAGGCTTGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGgtg < 1:1098831/69‑1 (MQ=255) gATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGtt > 1:508157/1‑71 (MQ=255) aTAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGttt > 1:3499719/1‑71 (MQ=255) aaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTga < 1:991775/42‑1 (MQ=255) aaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTa > 1:3533259/1‑71 (MQ=255) aaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGttttt < 1:1114170/69‑1 (MQ=255) aaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGttttt < 1:2589095/69‑1 (MQ=255) aTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTAcc < 1:3018038/71‑1 (MQ=255) ttAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgtg > 1:2952382/1‑71 (MQ=255) tAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACa < 1:3783319/41‑1 (MQ=255) aGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgtgg < 1:1441087/70‑1 (MQ=255) aGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTa < 1:2625455/45‑1 (MQ=255) aGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTa < 1:3777413/45‑1 (MQ=255) gCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTg < 1:3342894/49‑1 (MQ=255) gCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgtg > 1:172173/1‑63 (MQ=255) cAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGt > 1:2527548/1‑71 (MQ=255) cAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGt < 1:2697472/71‑1 (MQ=255) aaCGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGtt > 1:1441953/1‑71 (MQ=255) aCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTAc > 1:433353/1‑56 (MQ=255) aCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCgg > 1:3827355/1‑68 (MQ=255) aCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCgg > 1:1370214/1‑68 (MQ=255) aCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCgg > 1:702810/1‑68 (MQ=255) cgcTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTa < 1:177989/52‑1 (MQ=255) cTGGTGGCGAAGGCACCCTGACGACAATCTACTGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGtt < 1:3399400/70‑1 (MQ=255) tggtggCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGtttt > 1:3429698/1‑47 (MQ=255) tggtggCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGtttt > 1:2639621/1‑47 (MQ=255) gtggCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTccgc > 1:1249732/1‑71 (MQ=255) gtggCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTccg < 1:3309672/70‑1 (MQ=255) gtggCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTccg < 1:8150/70‑1 (MQ=255) ggCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgtg > 1:473384/1‑50 (MQ=255) gCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTAcc < 1:2887599/46‑1 (MQ=255) gCGAAGGCACCCTGACGACAATCTACGGGATTCTGGTGTTTTTAcc < 1:2892368/46‑1 (MQ=255) cGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCgccgcc < 1:718166/71‑1 (MQ=255) cGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCgccgcc < 1:939856/71‑1 (MQ=255) cGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCgccgcc > 1:2947938/1‑71 (MQ=255) agAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCgg > 1:1352860/2‑56 (MQ=255) gAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTc < 1:1877075/58‑1 (MQ=255) gAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCa < 1:1452325/59‑1 (MQ=255) aGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTg > 1:3430486/1‑70 (MQ=255) aGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGtt > 1:2787890/1‑60 (MQ=255) | ATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTG > minE/2087599‑2087730 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |