| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,089,817 | C→T | 16.6% | V99V (GTC→GTT) | yhaK → | predicted pirin‑related protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,089,817 | 0 | C | T | 16.6% | 154.1 / 21.7 | 66 | V99V (GTC→GTT) | yhaK | predicted pirin‑related protein |
| Reads supporting (aligned to +/- strand): ref base C (24/31); new base T (5/6); total (29/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
CGATAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCC > minE/2089748‑2089887 | cgATAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCa < 1:2993848/71‑1 (MQ=255) gATAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAg > 1:103253/1‑71 (MQ=255) aTAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGc < 1:3580230/71‑1 (MQ=255) aTAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGATGCTCTCTACCCAGCCGGGTGTc < 1:3327351/68‑1 (MQ=255) cGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCta > 1:3050564/1‑69 (MQ=255) aaGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGc > 1:2895826/1‑71 (MQ=255) aGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCg < 1:947625/71‑1 (MQ=255) aGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCg < 1:891320/71‑1 (MQ=255) ggCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCg > 1:2888190/1‑70 (MQ=255) ggCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCg > 1:2073582/1‑70 (MQ=255) aaTCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAaca > 1:2397963/1‑71 (MQ=255) aaTCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAaca < 1:2614307/71‑1 (MQ=255) aTGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAAt > 1:3905007/1‑71 (MQ=255) aTGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAAt > 1:3401915/1‑71 (MQ=255) tCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAAtct > 1:666983/1‑69 (MQ=255) tCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAAtct > 1:1618357/1‑69 (MQ=255) ccAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCaaa < 1:104719/71‑1 (MQ=255) ccAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCaaa < 1:2464578/71‑1 (MQ=255) cgcCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACa < 1:3382813/71‑1 (MQ=255) cgcCGGAGAAGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAAACTCAGCAAAGACa < 1:1897389/71‑1 (MQ=255) cGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAAc > 1:2399519/1‑71 (MQ=255) ggAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAAcc > 1:32083/1‑71 (MQ=255) gagaGGCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAACCg > 1:2049807/1‑71 (MQ=255) gagaGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCg > 1:397789/1‑71 (MQ=255) agagGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGt < 1:2475581/71‑1 (MQ=255) agagGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGt < 1:3416831/71‑1 (MQ=255) agagGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGt < 1:2213957/71‑1 (MQ=255) gagGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGtt < 1:1466268/71‑1 (MQ=255) gagGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGt > 1:3417615/1‑70 (MQ=255) agGCGTTGCTGCTCTCTACCCAGACGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGtt < 1:2901492/70‑1 (MQ=255) agGCGTTGCTGCTCTCAACCCAGCCGGGTGTTAGCTATAGCGAAcaca > 1:2544001/1‑48 (MQ=255) ggCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTaa < 1:3391440/71‑1 (MQ=255) gCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAAc > 1:3507646/1‑71 (MQ=255) gCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAAcc < 1:36612/65‑1 (MQ=255) tgctgcTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCATTAAcgcg > 1:3556392/1‑70 (MQ=255) ctgctCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGaa < 1:1658135/70‑1 (MQ=255) tgctCTCTACCCAGCCGGGTGTCAGCTATAGCGAacac < 1:2826845/38‑1 (MQ=255) ctctctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAAc > 1:343327/1‑62 (MQ=255) ctctctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCa < 1:2932554/71‑1 (MQ=255) tctctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATg < 1:2926509/68‑1 (MQ=255) tctACCCAGCCTGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAAc < 1:2187721/52‑1 (MQ=255) tctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCt > 1:3782505/1‑71 (MQ=255) ctACGCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCtt > 1:2946742/1‑71 (MQ=255) tACCGAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCttt > 1:3833780/1‑71 (MQ=255) agccAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCt < 1:2420439/66‑1 (MQ=255) cAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCaaa > 1:453900/1‑39 (MQ=255) cAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCaaa > 1:1819527/1‑39 (MQ=255) aGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTg < 1:3785620/71‑1 (MQ=255) aGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTg < 1:3255109/71‑1 (MQ=255) aGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACaa < 1:99827/43‑1 (MQ=255) aGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACaa < 1:16923/43‑1 (MQ=255) aGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTg > 1:990427/1‑71 (MQ=255) gCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACaaa < 1:2544334/43‑1 (MQ=255) gCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTg > 1:2685777/1‑70 (MQ=255) cGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCt < 1:394880/61‑1 (MQ=255) cGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGAc < 1:2647643/71‑1 (MQ=255) cGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGAc < 1:529779/71‑1 (MQ=255) cGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTaa > 1:1169207/1‑48 (MQ=255) cGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTaa > 1:2560408/1‑48 (MQ=255) cGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGAc < 1:2416198/71‑1 (MQ=255) ggTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAAc > 1:3891675/1‑40 (MQ=255) tgtCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGc < 1:181834/69‑1 (MQ=255) tCAGCTATAGCGAACACAATCTCAGCAAAGACAAAc < 1:3147385/36‑1 (MQ=255) tCAGCTATAGCGAACACAATCTCAGCAAAGACAAAc < 1:2798741/36‑1 (MQ=255) tCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTa < 1:1762658/41‑1 (MQ=255) tCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGc < 1:666722/71‑1 (MQ=255) tCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGc < 1:1395614/71‑1 (MQ=255) cAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAAt > 1:544477/1‑48 (MQ=255) cAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGcc > 1:636301/1‑71 (MQ=255) cAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGAGAATGCAGCTTTGGCTGGACGCCTGcc > 1:2503837/1‑71 (MQ=255) | CGATAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCC > minE/2089748‑2089887 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |