Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,093,664 A→G 6.2% N396N (AAT→AAC)  tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,093,6640AG 6.2% 227.6 / 5.1 81N396N (AAT→AAC) tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base A (47/29);  new base G (2/3);  total (49/32)
Fisher's exact test for biased strand distribution p-value = 3.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

AACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCA  >  minE/2093595‑2093734
                                                                     |                                                                      
aaCACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCAt                                                                       >  1:3887885/1‑71 (MQ=255)
   acGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCAtt                                                                      >  1:3706645/1‑69 (MQ=255)
   acGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCAtt                                                                      >  1:3711166/1‑69 (MQ=255)
   acGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTAc                                                                    >  1:1289487/1‑71 (MQ=255)
    cgcgGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACg                                                                   <  1:3776976/71‑1 (MQ=255)
    cgcgGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACg                                                                   <  1:3893472/71‑1 (MQ=255)
       ggTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGtt                                                                 >  1:296519/1‑70 (MQ=255)
         tGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCAt                                                                       <  1:615157/62‑1 (MQ=255)
          gCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCa                                                               >  1:901598/1‑69 (MQ=255)
             gAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                                              >  1:1181378/1‑67 (MQ=255)
               ggTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACg                                                        >  1:747704/1‑71 (MQ=255)
                gTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACgg                                                       <  1:3225755/71‑1 (MQ=255)
                   cggcgCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAt                                                    >  1:8963/1‑71 (MQ=255)
                         aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                                                <  1:796598/53‑1 (MQ=255)
                         aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                              >  1:3745743/1‑71 (MQ=255)
                         aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                              <  1:6982/71‑1 (MQ=255)
                         aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                              >  1:2485069/1‑71 (MQ=255)
                           cGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                            >  1:168311/1‑71 (MQ=255)
                           cGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                            >  1:3399835/1‑71 (MQ=255)
                           cGCCATCCGCGCGGAGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                            >  1:3222038/1‑71 (MQ=255)
                            gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                                           >  1:1567766/1‑71 (MQ=255)
                              cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                            <  1:3566136/68‑1 (MQ=255)
                              cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                            <  1:101659/68‑1 (MQ=255)
                              cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                         <  1:3595496/71‑1 (MQ=255)
                              cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                         <  1:3664100/71‑1 (MQ=255)
                               aTCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTcc                                        >  1:1921021/1‑71 (MQ=255)
                                tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTcc                                        <  1:3503917/70‑1 (MQ=255)
                                tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCg                                       <  1:2604308/71‑1 (MQ=255)
                                 ccGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGtt                                                                 >  1:222395/1‑44 (MQ=255)
                                  cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTAc                                                                    >  1:578433/1‑40 (MQ=255)
                                  cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                      >  1:481807/1‑70 (MQ=255)
                                    cgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCa                                                               >  1:2639943/1‑43 (MQ=255)
                                     gcggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                                                  >  1:1516098/1‑55 (MQ=255)
                                     gcggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCaa                                   <  1:1269140/70‑1 (MQ=255)
                                      cggcgTTTACTGCTTTCACGGCATTAATGGCATTACGtt                                                                 <  1:1105301/39‑1 (MQ=255)
                                       ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg                                <  1:1470898/71‑1 (MQ=255)
                                        gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCa                                                               >  1:55805/1‑39 (MQ=255)
                                        gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                               <  1:2104486/71‑1 (MQ=255)
                                         cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                               <  1:594042/70‑1 (MQ=255)
                                         cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                               <  1:3166183/70‑1 (MQ=255)
                                         cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGa                              >  1:842131/1‑71 (MQ=255)
                                           tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                             >  1:2542348/1‑70 (MQ=255)
                                           tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATc                            <  1:457663/71‑1 (MQ=255)
                                           tttACTGCTTTCACGGCATTAATGGAATTACGTTCAATGCAAGGGATTTGTACCTGTCCGGCAACCGGATc                            >  1:1050312/1‑71 (MQ=255)
                                             tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                           >  1:2922620/1‑70 (MQ=255)
                                             tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                           >  1:2917917/1‑70 (MQ=255)
                                             tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                           >  1:2426003/1‑70 (MQ=255)
                                             tACTGCTTTCACGGCATTAATGGCATTACGTTCAACGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                           >  1:3823908/1‑70 (MQ=255)
                                               cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                              >  1:1282288/1‑49 (MQ=255)
                                               cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                              >  1:2207141/1‑49 (MQ=255)
                                               cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                        >  1:980117/1‑71 (MQ=255)
                                                tGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAgg                       >  1:65814/1‑71 (MQ=255)
                                                 gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                         <  1:1713412/52‑1 (MQ=255)
                                                 gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                         <  1:1188368/52‑1 (MQ=255)
                                                 gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                      >  1:2113141/1‑71 (MQ=255)
                                                  cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                      <  1:3682707/70‑1 (MQ=255)
                                                   tttCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                                                  >  1:1893355/1‑41 (MQ=255)
                                                   tttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCg                                       >  1:3942410/1‑52 (MQ=255)
                                                    ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCa                    >  1:2099797/1‑70 (MQ=255)
                                                    ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                   >  1:1688047/1‑71 (MQ=255)
                                                    ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                   >  1:2115135/1‑71 (MQ=255)
                                                     tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                        >  1:2729925/1‑65 (MQ=255)
                                                     tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc                  >  1:3463932/1‑71 (MQ=255)
                                                       aaggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGcc                 >  1:3397224/3‑70 (MQ=255)
                                                        cGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa               <  1:1339931/71‑1 (MQ=255)
                                                          gCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg             >  1:907870/1‑71 (MQ=255)
                                                           cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                           >  1:1891197/1‑56 (MQ=255)
                                                           cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                           >  1:3647834/1‑56 (MQ=255)
                                                           cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg            <  1:2020089/71‑1 (MQ=255)
                                                            aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg            >  1:3659780/1‑70 (MQ=255)
                                                             ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCaa                                   <  1:2031837/46‑1 (MQ=255)
                                                             ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGtt          <  1:2367230/71‑1 (MQ=255)
                                                             gcaaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGCCCGAg             >  1:1679234/3‑68 (MQ=255)
                                                              tAATGGCATTACGTTCAATGCACGGGATTTGTTCCTGTCCGGCAACCGGATCGCAGGt                      <  1:447430/58‑1 (MQ=255)
                                                              caaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGttg         <  1:476134/70‑2 (MQ=255)
                                                                aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa         <  1:1192641/69‑1 (MQ=255)
                                                                aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt        <  1:2990572/70‑1 (MQ=255)
                                                                 tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                   <  1:572740/58‑1 (MQ=255)
                                                                 tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                   <  1:1916719/58‑1 (MQ=255)
                                                                  ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt     >  1:2251416/1‑71 (MQ=255)
                                                                   gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg            >  1:2302445/1‑63 (MQ=255)
                                                                     aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa  <  1:2510351/71‑1 (MQ=255)
                                                                     aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa  <  1:2041893/71‑1 (MQ=255)
                                                                     |                                                                      
AACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCA  >  minE/2093595‑2093734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑