Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,093,670	T→C	10.7%	E394E (GAA→GAG)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,093,670	0	T	C	10.7%	227.5 / 15.7	84	E394E (GAA→GAG)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base T (45/30);  new base C (5/4);  total (50/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

GGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCG  >  minE/2093602‑2093739                                                                     |                                                                      ggTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGtt                                                                      >  1:296519/1‑70 (MQ=255)    gCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCa                                                                    >  1:901598/1‑69 (MQ=255)       gAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                                                   >  1:1181378/1‑67 (MQ=255)         ggTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACg                                                             >  1:747704/1‑71 (MQ=255)          gTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACgg                                                            <  1:3225755/71‑1 (MQ=255)             cggcgCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAt                                                         >  1:8963/1‑71 (MQ=255)                   aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                                                     <  1:796598/53‑1 (MQ=255)                   aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                   >  1:2485069/1‑71 (MQ=255)                   aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                   >  1:3745743/1‑71 (MQ=255)                   aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                   <  1:6982/71‑1 (MQ=255)                     cGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                 >  1:3399835/1‑71 (MQ=255)                     cGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                 >  1:168311/1‑71 (MQ=255)                     cGCCATCCGCGCGGAGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                 >  1:3222038/1‑71 (MQ=255)                      gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                                                >  1:1567766/1‑71 (MQ=255)                        cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                 <  1:101659/68‑1 (MQ=255)                        cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                 <  1:3566136/68‑1 (MQ=255)                        cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                              <  1:3595496/71‑1 (MQ=255)                        cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                              <  1:3664100/71‑1 (MQ=255)                         aTCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTcc                                             >  1:1921021/1‑71 (MQ=255)                          tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTcc                                             <  1:3503917/70‑1 (MQ=255)                          tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCg                                            <  1:2604308/71‑1 (MQ=255)                           ccGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGtt                                                                      >  1:222395/1‑44 (MQ=255)                            cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                           >  1:481807/1‑70 (MQ=255)                              cgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCa                                                                    >  1:2639943/1‑43 (MQ=255)                               gcggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                                                       >  1:1516098/1‑55 (MQ=255)                               gcggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCaa                                        <  1:1269140/70‑1 (MQ=255)                                cggcgTTTACTGCTTTCACGGCATTAATGGCATTACGtt                                                                      <  1:1105301/39‑1 (MQ=255)                                 ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg                                     <  1:1470898/71‑1 (MQ=255)                                  gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCa                                                                    >  1:55805/1‑39 (MQ=255)                                  gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                    <  1:2104486/71‑1 (MQ=255)                                   cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                    <  1:594042/70‑1 (MQ=255)                                   cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                    <  1:3166183/70‑1 (MQ=255)                                   cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGa                                   >  1:842131/1‑71 (MQ=255)                                     tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                  >  1:2542348/1‑70 (MQ=255)                                     tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATc                                 <  1:457663/71‑1 (MQ=255)                                     tttACTGCTTTCACGGCATTAATGGAATTACGTTCAATGCAAGGGATTTGTACCTGTCCGGCAACCGGATc                                 >  1:1050312/1‑71 (MQ=255)                                       tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                >  1:2426003/1‑70 (MQ=255)                                       tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                >  1:2922620/1‑70 (MQ=255)                                       tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                >  1:2917917/1‑70 (MQ=255)                                       tACTGCTTTCACGGCATTAATGGCATTACGTTCAACGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                >  1:3823908/1‑70 (MQ=255)                                         cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                   >  1:2207141/1‑49 (MQ=255)                                         cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                   >  1:1282288/1‑49 (MQ=255)                                         cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                             >  1:980117/1‑71 (MQ=255)                                          tGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAgg                            >  1:65814/1‑71 (MQ=255)                                           gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                              <  1:1188368/52‑1 (MQ=255)                                           gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                              <  1:1713412/52‑1 (MQ=255)                                           gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                           >  1:2113141/1‑71 (MQ=255)                                            cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                           <  1:3682707/70‑1 (MQ=255)                                             tttCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                                                       >  1:1893355/1‑41 (MQ=255)                                             tttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCg                                            >  1:3942410/1‑52 (MQ=255)                                              ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCa                         >  1:2099797/1‑70 (MQ=255)                                              ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                        >  1:1688047/1‑71 (MQ=255)                                              ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                        >  1:2115135/1‑71 (MQ=255)                                               tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                             >  1:2729925/1‑65 (MQ=255)                                               tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc                       >  1:3463932/1‑71 (MQ=255)                                                 aaggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGcc                      >  1:3397224/3‑70 (MQ=255)                                                  cGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                    <  1:1339931/71‑1 (MQ=255)                                                    gCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                  >  1:907870/1‑71 (MQ=255)                                                     cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                >  1:1891197/1‑56 (MQ=255)                                                     cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                >  1:3647834/1‑56 (MQ=255)                                                     cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                 <  1:2020089/71‑1 (MQ=255)                                                      aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                 >  1:3659780/1‑70 (MQ=255)                                                       ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCaa                                        <  1:2031837/46‑1 (MQ=255)                                                       ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGtt               <  1:2367230/71‑1 (MQ=255)                                                       gcaaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGCCCGAg                  >  1:1679234/3‑68 (MQ=255)                                                        tAATGGCATTACGTTCAATGCACGGGATTTGTTCCTGTCCGGCAACCGGATCGCAGGt                           <  1:447430/58‑1 (MQ=255)                                                        caaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGttg              <  1:476134/70‑2 (MQ=255)                                                          aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa              <  1:1192641/69‑1 (MQ=255)                                                          aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt             <  1:2990572/70‑1 (MQ=255)                                                           tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                        <  1:1916719/58‑1 (MQ=255)                                                           tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                        <  1:572740/58‑1 (MQ=255)                                                            ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt          >  1:2251416/1‑71 (MQ=255)                                                             gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                 >  1:2302445/1‑63 (MQ=255)                                                               gttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGCGTCACACGTCAg                        <  1:2645771/53‑1 (MQ=255)                                                               aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa       <  1:2510351/71‑1 (MQ=255)                                                               aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa       <  1:2041893/71‑1 (MQ=255)                                                                 tACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtg     >  1:32290/1‑70 (MQ=255)                                                                  aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                   >  1:3553785/1‑56 (MQ=255)                                                                  aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa              >  1:3738059/1‑61 (MQ=255)                                                                  aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc         >  1:2898859/1‑66 (MQ=255)                                                                  aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg    >  1:432397/1‑69 (MQ=255)                                                                  aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg    >  1:3945273/1‑69 (MQ=255)                                                                   cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                     >  1:54267/1‑53 (MQ=255)                                                                   cGTTCAATGCACGGGATATGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                     >  1:935363/1‑53 (MQ=255)                                                                    gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgcg  <  1:1594202/71‑1 (MQ=255)                                                                    gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg    <  1:2607773/69‑1 (MQ=255)                                                                    gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg    <  1:1090986/69‑1 (MQ=255)                                                                     |                                                                      GGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCG  >  minE/2093602‑2093739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑