Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,093,673	A→G	11.4%	I393I (ATT→ATC)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,093,673	0	A	G	11.4%	211.2 / 16.0	79	I393I (ATT→ATC)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base A (41/29);  new base G (5/4);  total (46/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATT  >  minE/2093605‑2093742                                                                     |                                                                      gCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCa                                                                       >  1:901598/1‑69 (MQ=255)    gAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                                                      >  1:1181378/1‑67 (MQ=255)      ggTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACg                                                                >  1:747704/1‑71 (MQ=255)       gTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACgg                                                               <  1:3225755/71‑1 (MQ=255)          cggcgCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAt                                                            >  1:8963/1‑71 (MQ=255)                aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                      >  1:3745743/1‑71 (MQ=255)                aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                      >  1:2485069/1‑71 (MQ=255)                aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                      <  1:6982/71‑1 (MQ=255)                  cGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                    >  1:3399835/1‑71 (MQ=255)                  cGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                    >  1:168311/1‑71 (MQ=255)                  cGCCATCCGCGCGGAGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                    >  1:3222038/1‑71 (MQ=255)                   gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                                                   >  1:1567766/1‑71 (MQ=255)                     cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                    <  1:3566136/68‑1 (MQ=255)                     cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                    <  1:101659/68‑1 (MQ=255)                     cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                 <  1:3664100/71‑1 (MQ=255)                     cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                 <  1:3595496/71‑1 (MQ=255)                      aTCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTcc                                                >  1:1921021/1‑71 (MQ=255)                       tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTcc                                                <  1:3503917/70‑1 (MQ=255)                       tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCg                                               <  1:2604308/71‑1 (MQ=255)                         cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                              >  1:481807/1‑70 (MQ=255)                           cgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCa                                                                       >  1:2639943/1‑43 (MQ=255)                            gcggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                                                          >  1:1516098/1‑55 (MQ=255)                            gcggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCaa                                           <  1:1269140/70‑1 (MQ=255)                              ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg                                        <  1:1470898/71‑1 (MQ=255)                               gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCa                                                                       >  1:55805/1‑39 (MQ=255)                               gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                       <  1:2104486/71‑1 (MQ=255)                                cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                       <  1:594042/70‑1 (MQ=255)                                cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                       <  1:3166183/70‑1 (MQ=255)                                cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGa                                      >  1:842131/1‑71 (MQ=255)                                  tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                     >  1:2542348/1‑70 (MQ=255)                                  tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATc                                    <  1:457663/71‑1 (MQ=255)                                  tttACTGCTTTCACGGCATTAATGGAATTACGTTCAATGCAAGGGATTTGTACCTGTCCGGCAACCGGATc                                    >  1:1050312/1‑71 (MQ=255)                                    tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                   >  1:2922620/1‑70 (MQ=255)                                    tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                   >  1:2917917/1‑70 (MQ=255)                                    tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                   >  1:2426003/1‑70 (MQ=255)                                    tACTGCTTTCACGGCATTAATGGCATTACGTTCAACGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                   >  1:3823908/1‑70 (MQ=255)                                      cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                      >  1:2207141/1‑49 (MQ=255)                                      cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                      >  1:1282288/1‑49 (MQ=255)                                      cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                >  1:980117/1‑71 (MQ=255)                                       tGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAgg                               >  1:65814/1‑71 (MQ=255)                                        gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                 <  1:1188368/52‑1 (MQ=255)                                        gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                 <  1:1713412/52‑1 (MQ=255)                                        gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                              >  1:2113141/1‑71 (MQ=255)                                         cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                              <  1:3682707/70‑1 (MQ=255)                                          tttCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                                                          >  1:1893355/1‑41 (MQ=255)                                          tttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCg                                               >  1:3942410/1‑52 (MQ=255)                                           ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCa                            >  1:2099797/1‑70 (MQ=255)                                           ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                           >  1:2115135/1‑71 (MQ=255)                                           ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                           >  1:1688047/1‑71 (MQ=255)                                            tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                >  1:2729925/1‑65 (MQ=255)                                            tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc                          >  1:3463932/1‑71 (MQ=255)                                              aaggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGcc                         >  1:3397224/3‑70 (MQ=255)                                               cGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                       <  1:1339931/71‑1 (MQ=255)                                                 gCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                     >  1:907870/1‑71 (MQ=255)                                                  cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                   >  1:1891197/1‑56 (MQ=255)                                                  cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                   >  1:3647834/1‑56 (MQ=255)                                                  cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                    <  1:2020089/71‑1 (MQ=255)                                                   aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                    >  1:3659780/1‑70 (MQ=255)                                                    ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCaa                                           <  1:2031837/46‑1 (MQ=255)                                                    ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGtt                  <  1:2367230/71‑1 (MQ=255)                                                    gcaaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGCCCGAg                     >  1:1679234/3‑68 (MQ=255)                                                     tAATGGCATTACGTTCAATGCACGGGATTTGTTCCTGTCCGGCAACCGGATCGCAGGt                              <  1:447430/58‑1 (MQ=255)                                                     caaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGttg                 <  1:476134/70‑2 (MQ=255)                                                       aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                 <  1:1192641/69‑1 (MQ=255)                                                       aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                <  1:2990572/70‑1 (MQ=255)                                                        tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                           <  1:572740/58‑1 (MQ=255)                                                        tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                           <  1:1916719/58‑1 (MQ=255)                                                         ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt             >  1:2251416/1‑71 (MQ=255)                                                          gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                    >  1:2302445/1‑63 (MQ=255)                                                            gttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGCGTCACACGTCAg                           <  1:2645771/53‑1 (MQ=255)                                                            aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa          <  1:2041893/71‑1 (MQ=255)                                                            aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa          <  1:2510351/71‑1 (MQ=255)                                                              tACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtg        >  1:32290/1‑70 (MQ=255)                                                               aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                      >  1:3553785/1‑56 (MQ=255)                                                               aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                 >  1:3738059/1‑61 (MQ=255)                                                               aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc            >  1:2898859/1‑66 (MQ=255)                                                               aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg       >  1:432397/1‑69 (MQ=255)                                                               aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg       >  1:3945273/1‑69 (MQ=255)                                                                cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                        >  1:54267/1‑53 (MQ=255)                                                                cGTTCAATGCACGGGATATGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                        >  1:935363/1‑53 (MQ=255)                                                                 gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgcg     <  1:1594202/71‑1 (MQ=255)                                                                 gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg       <  1:2607773/69‑1 (MQ=255)                                                                 gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg       <  1:1090986/69‑1 (MQ=255)                                                                     aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt  <  1:1713354/70‑1 (MQ=255)                                                                     aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt  <  1:1763668/70‑1 (MQ=255)                                                                     |                                                                      GCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATT  >  minE/2093605‑2093742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑