Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,093,679	C→T	10.1%	P391P (CCG→CCA)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,093,679	0	C	T	10.1%	252.9 / 14.2	89	P391P (CCG→CCA)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base C (44/35);  new base T (5/4);  total (50/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGC  >  minE/2093610‑2093747                                                                      |                                                                     ggTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACg                                                                     >  1:747704/1‑71 (MQ=255)  gTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACgg                                                                    <  1:3225755/71‑1 (MQ=255)     cggcgCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAt                                                                 >  1:8963/1‑71 (MQ=255)           aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                           >  1:2485069/1‑71 (MQ=255)           aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                           <  1:6982/71‑1 (MQ=255)           aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                           >  1:3745743/1‑71 (MQ=255)             cGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                         >  1:3399835/1‑71 (MQ=255)             cGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                         >  1:168311/1‑71 (MQ=255)             cGCCATCCGCGCGGAGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                         >  1:3222038/1‑71 (MQ=255)              gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                                                        >  1:1567766/1‑71 (MQ=255)                cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                         <  1:101659/68‑1 (MQ=255)                cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                         <  1:3566136/68‑1 (MQ=255)                cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                      <  1:3595496/71‑1 (MQ=255)                cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                      <  1:3664100/71‑1 (MQ=255)                 aTCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTcc                                                     >  1:1921021/1‑71 (MQ=255)                  tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTcc                                                     <  1:3503917/70‑1 (MQ=255)                  tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCg                                                    <  1:2604308/71‑1 (MQ=255)                    cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                                   >  1:481807/1‑70 (MQ=255)                       gcggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                                                               >  1:1516098/1‑55 (MQ=255)                       gcggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCaa                                                <  1:1269140/70‑1 (MQ=255)                         ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg                                             <  1:1470898/71‑1 (MQ=255)                          gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                            <  1:2104486/71‑1 (MQ=255)                           cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                            <  1:594042/70‑1 (MQ=255)                           cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                            <  1:3166183/70‑1 (MQ=255)                           cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGa                                           >  1:842131/1‑71 (MQ=255)                             tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                          >  1:2542348/1‑70 (MQ=255)                             tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATc                                         <  1:457663/71‑1 (MQ=255)                             tttACTGCTTTCACGGCATTAATGGAATTACGTTCAATGCAAGGGATTTGTACCTGTCCGGCAACCGGATc                                         >  1:1050312/1‑71 (MQ=255)                               tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                        >  1:2917917/1‑70 (MQ=255)                               tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                        >  1:2922620/1‑70 (MQ=255)                               tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                        >  1:2426003/1‑70 (MQ=255)                               tACTGCTTTCACGGCATTAATGGCATTACGTTCAACGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                        >  1:3823908/1‑70 (MQ=255)                                 cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                           >  1:1282288/1‑49 (MQ=255)                                 cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                           >  1:2207141/1‑49 (MQ=255)                                 cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                     >  1:980117/1‑71 (MQ=255)                                  tGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAgg                                    >  1:65814/1‑71 (MQ=255)                                   gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                      <  1:1713412/52‑1 (MQ=255)                                   gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                      <  1:1188368/52‑1 (MQ=255)                                   gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                   >  1:2113141/1‑71 (MQ=255)                                    cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                   <  1:3682707/70‑1 (MQ=255)                                     tttCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                                                               >  1:1893355/1‑41 (MQ=255)                                     tttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCg                                                    >  1:3942410/1‑52 (MQ=255)                                      ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCa                                 >  1:2099797/1‑70 (MQ=255)                                      ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                >  1:1688047/1‑71 (MQ=255)                                      ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                >  1:2115135/1‑71 (MQ=255)                                       tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                     >  1:2729925/1‑65 (MQ=255)                                       tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc                               >  1:3463932/1‑71 (MQ=255)                                         aaggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGcc                              >  1:3397224/3‑70 (MQ=255)                                          cGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                            <  1:1339931/71‑1 (MQ=255)                                            gCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                          >  1:907870/1‑71 (MQ=255)                                             cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                        >  1:3647834/1‑56 (MQ=255)                                             cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                        >  1:1891197/1‑56 (MQ=255)                                             cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                         <  1:2020089/71‑1 (MQ=255)                                              aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                         >  1:3659780/1‑70 (MQ=255)                                               ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCaa                                                <  1:2031837/46‑1 (MQ=255)                                               ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGtt                       <  1:2367230/71‑1 (MQ=255)                                               gcaaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGCCCGAg                          >  1:1679234/3‑68 (MQ=255)                                                tAATGGCATTACGTTCAATGCACGGGATTTGTTCCTGTCCGGCAACCGGATCGCAGGt                                   <  1:447430/58‑1 (MQ=255)                                                caaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGttg                      <  1:476134/70‑2 (MQ=255)                                                  aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                      <  1:1192641/69‑1 (MQ=255)                                                  aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                     <  1:2990572/70‑1 (MQ=255)                                                   tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                                <  1:1916719/58‑1 (MQ=255)                                                   tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                                <  1:572740/58‑1 (MQ=255)                                                    ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                  >  1:2251416/1‑71 (MQ=255)                                                     gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                         >  1:2302445/1‑63 (MQ=255)                                                       gttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGCGTCACACGTCAg                                <  1:2645771/53‑1 (MQ=255)                                                       aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa               <  1:2510351/71‑1 (MQ=255)                                                       aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa               <  1:2041893/71‑1 (MQ=255)                                                         tACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtg             >  1:32290/1‑70 (MQ=255)                                                          aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                           >  1:3553785/1‑56 (MQ=255)                                                          aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                      >  1:3738059/1‑61 (MQ=255)                                                          aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                 >  1:2898859/1‑66 (MQ=255)                                                          aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg            >  1:3945273/1‑69 (MQ=255)                                                          aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg            >  1:432397/1‑69 (MQ=255)                                                           cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                             >  1:54267/1‑53 (MQ=255)                                                           cGTTCAATGCACGGGATATGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                             >  1:935363/1‑53 (MQ=255)                                                            gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgcg          <  1:1594202/71‑1 (MQ=255)                                                            gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg            <  1:1090986/69‑1 (MQ=255)                                                            gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg            <  1:2607773/69‑1 (MQ=255)                                                                aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt       <  1:1713354/70‑1 (MQ=255)                                                                aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt       <  1:1763668/70‑1 (MQ=255)                                                                  tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc    <  1:3704535/71‑1 (MQ=255)                                                                   gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                >  1:3031072/1‑58 (MQ=255)                                                                   gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa               >  1:1203666/1‑59 (MQ=255)                                                                    cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                     >  1:1209205/1‑36 (MQ=255)                                                                    cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                      >  1:1182642/1‑51 (MQ=255)                                                                    cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc  <  1:482353/71‑1 (MQ=255)                                                                    cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc  <  1:327095/71‑1 (MQ=255)                                                                    cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccg   <  1:385379/70‑1 (MQ=255)                                                                      cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc     <  1:2370356/66‑1 (MQ=255)                                                                      |                                                                     GGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGC  >  minE/2093610‑2093747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑