Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,093,682 G→C 10.2% I390M (ATC→ATG) ‡ tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,093,6820GC10.2% 241.5 / 15.0 88I390M (ATC→ATG) ‡tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base G (44/35);  new base C (5/4);  total (49/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

CGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGC  >  minE/2093614‑2093747
                                                                    |                                                                 
cggcgCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAt                                                                 >  1:8963/1‑71 (MQ=255)
      aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                           >  1:2485069/1‑71 (MQ=255)
      aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                           <  1:6982/71‑1 (MQ=255)
      aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                           >  1:3745743/1‑71 (MQ=255)
        cGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                         >  1:3399835/1‑71 (MQ=255)
        cGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                         >  1:168311/1‑71 (MQ=255)
        cGCCATCCGCGCGGAGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                         >  1:3222038/1‑71 (MQ=255)
         gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                                                        >  1:1567766/1‑71 (MQ=255)
           cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                         <  1:101659/68‑1 (MQ=255)
           cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                         <  1:3566136/68‑1 (MQ=255)
           cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                      <  1:3595496/71‑1 (MQ=255)
           cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                      <  1:3664100/71‑1 (MQ=255)
            aTCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTcc                                                     >  1:1921021/1‑71 (MQ=255)
             tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTcc                                                     <  1:3503917/70‑1 (MQ=255)
             tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCg                                                    <  1:2604308/71‑1 (MQ=255)
               cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                                   >  1:481807/1‑70 (MQ=255)
                  gcggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                                                               >  1:1516098/1‑55 (MQ=255)
                  gcggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCaa                                                <  1:1269140/70‑1 (MQ=255)
                    ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg                                             <  1:1470898/71‑1 (MQ=255)
                     gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                            <  1:2104486/71‑1 (MQ=255)
                      cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                            <  1:594042/70‑1 (MQ=255)
                      cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                            <  1:3166183/70‑1 (MQ=255)
                      cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGa                                           >  1:842131/1‑71 (MQ=255)
                        tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                          >  1:2542348/1‑70 (MQ=255)
                        tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATc                                         <  1:457663/71‑1 (MQ=255)
                        tttACTGCTTTCACGGCATTAATGGAATTACGTTCAATGCAAGGGATTTGTACCTGTCCGGCAACCGGATc                                         >  1:1050312/1‑71 (MQ=255)
                          tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                        >  1:2917917/1‑70 (MQ=255)
                          tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                        >  1:2922620/1‑70 (MQ=255)
                          tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                        >  1:2426003/1‑70 (MQ=255)
                          tACTGCTTTCACGGCATTAATGGCATTACGTTCAACGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                        >  1:3823908/1‑70 (MQ=255)
                            cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                           >  1:1282288/1‑49 (MQ=255)
                            cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                           >  1:2207141/1‑49 (MQ=255)
                            cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                     >  1:980117/1‑71 (MQ=255)
                             tGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAgg                                    >  1:65814/1‑71 (MQ=255)
                              gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                      <  1:1713412/52‑1 (MQ=255)
                              gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                      <  1:1188368/52‑1 (MQ=255)
                              gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                   >  1:2113141/1‑71 (MQ=255)
                               cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                   <  1:3682707/70‑1 (MQ=255)
                                tttCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                                                               >  1:1893355/1‑41 (MQ=255)
                                tttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCg                                                    >  1:3942410/1‑52 (MQ=255)
                                 ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCa                                 >  1:2099797/1‑70 (MQ=255)
                                 ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                >  1:2115135/1‑71 (MQ=255)
                                 ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                >  1:1688047/1‑71 (MQ=255)
                                  tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                     >  1:2729925/1‑65 (MQ=255)
                                  tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc                               >  1:3463932/1‑71 (MQ=255)
                                    aaggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGcc                              >  1:3397224/3‑70 (MQ=255)
                                     cGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                            <  1:1339931/71‑1 (MQ=255)
                                       gCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                          >  1:907870/1‑71 (MQ=255)
                                        cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                        >  1:3647834/1‑56 (MQ=255)
                                        cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                        >  1:1891197/1‑56 (MQ=255)
                                        cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                         <  1:2020089/71‑1 (MQ=255)
                                         aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                         >  1:3659780/1‑70 (MQ=255)
                                          ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCaa                                                <  1:2031837/46‑1 (MQ=255)
                                          ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGtt                       <  1:2367230/71‑1 (MQ=255)
                                          gcaaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGCCCGAg                          >  1:1679234/3‑68 (MQ=255)
                                           tAATGGCATTACGTTCAATGCACGGGATTTGTTCCTGTCCGGCAACCGGATCGCAGGt                                   <  1:447430/58‑1 (MQ=255)
                                           caaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGttg                      <  1:476134/70‑2 (MQ=255)
                                             aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                      <  1:1192641/69‑1 (MQ=255)
                                             aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                     <  1:2990572/70‑1 (MQ=255)
                                              tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                                <  1:572740/58‑1 (MQ=255)
                                              tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                                <  1:1916719/58‑1 (MQ=255)
                                               ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                  >  1:2251416/1‑71 (MQ=255)
                                                gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                         >  1:2302445/1‑63 (MQ=255)
                                                  gttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGCGTCACACGTCAg                                <  1:2645771/53‑1 (MQ=255)
                                                  aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa               <  1:2510351/71‑1 (MQ=255)
                                                  aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa               <  1:2041893/71‑1 (MQ=255)
                                                    tACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtg             >  1:32290/1‑70 (MQ=255)
                                                     aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                           >  1:3553785/1‑56 (MQ=255)
                                                     aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                      >  1:3738059/1‑61 (MQ=255)
                                                     aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                 >  1:2898859/1‑66 (MQ=255)
                                                     aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg            >  1:3945273/1‑69 (MQ=255)
                                                     aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg            >  1:432397/1‑69 (MQ=255)
                                                      cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                             >  1:54267/1‑53 (MQ=255)
                                                      cGTTCAATGCACGGGATATGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                             >  1:935363/1‑53 (MQ=255)
                                                       gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgcg          <  1:1594202/71‑1 (MQ=255)
                                                       gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg            <  1:1090986/69‑1 (MQ=255)
                                                       gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg            <  1:2607773/69‑1 (MQ=255)
                                                           aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt       <  1:1713354/70‑1 (MQ=255)
                                                           aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt       <  1:1763668/70‑1 (MQ=255)
                                                             tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc    <  1:3704535/71‑1 (MQ=255)
                                                              gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                >  1:3031072/1‑58 (MQ=255)
                                                              gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa               >  1:1203666/1‑59 (MQ=255)
                                                               cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                     >  1:1209205/1‑36 (MQ=255)
                                                               cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                      >  1:1182642/1‑51 (MQ=255)
                                                               cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc  <  1:482353/71‑1 (MQ=255)
                                                               cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc  <  1:327095/71‑1 (MQ=255)
                                                               cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccg   <  1:385379/70‑1 (MQ=255)
                                                                 cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc     <  1:2370356/66‑1 (MQ=255)
                                                                   ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg            >  1:3059344/1‑57 (MQ=255)
                                                                    |                                                                 
CGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGC  >  minE/2093614‑2093747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑