Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,093,684	T→C	10.1%	I390V (ATC→GTC) ‡	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,093,684	0	T	C	10.1%	241.6 / 15.3	89	I390V (ATC→GTC) ‡	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base T (43/37);  new base C (5/4);  total (48/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

CGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATT  >  minE/2093614‑2093753                                                                       |                                                                      cggcgCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAt                                                                       >  1:8963/1‑71 (MQ=255)       aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                                 >  1:3745743/1‑71 (MQ=255)       aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                                 <  1:6982/71‑1 (MQ=255)       aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                                 >  1:2485069/1‑71 (MQ=255)         cGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                               >  1:168311/1‑71 (MQ=255)         cGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                               >  1:3399835/1‑71 (MQ=255)         cGCCATCCGCGCGGAGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                               >  1:3222038/1‑71 (MQ=255)          gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                                                              >  1:1567766/1‑71 (MQ=255)            cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                               <  1:101659/68‑1 (MQ=255)            cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                               <  1:3566136/68‑1 (MQ=255)            cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                            <  1:3664100/71‑1 (MQ=255)            cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                            <  1:3595496/71‑1 (MQ=255)             aTCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTcc                                                           >  1:1921021/1‑71 (MQ=255)              tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTcc                                                           <  1:3503917/70‑1 (MQ=255)              tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCg                                                          <  1:2604308/71‑1 (MQ=255)                cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                                         >  1:481807/1‑70 (MQ=255)                   gcggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                                                                     >  1:1516098/1‑55 (MQ=255)                   gcggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCaa                                                      <  1:1269140/70‑1 (MQ=255)                     ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg                                                   <  1:1470898/71‑1 (MQ=255)                      gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                                  <  1:2104486/71‑1 (MQ=255)                       cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                                  <  1:594042/70‑1 (MQ=255)                       cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                                  <  1:3166183/70‑1 (MQ=255)                       cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGa                                                 >  1:842131/1‑71 (MQ=255)                         tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                                >  1:2542348/1‑70 (MQ=255)                         tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATc                                               <  1:457663/71‑1 (MQ=255)                         tttACTGCTTTCACGGCATTAATGGAATTACGTTCAATGCAAGGGATTTGTACCTGTCCGGCAACCGGATc                                               >  1:1050312/1‑71 (MQ=255)                           tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                              >  1:2917917/1‑70 (MQ=255)                           tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                              >  1:2922620/1‑70 (MQ=255)                           tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                              >  1:2426003/1‑70 (MQ=255)                           tACTGCTTTCACGGCATTAATGGCATTACGTTCAACGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                              >  1:3823908/1‑70 (MQ=255)                             cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                                 >  1:1282288/1‑49 (MQ=255)                             cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                                 >  1:2207141/1‑49 (MQ=255)                             cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                           >  1:980117/1‑71 (MQ=255)                              tGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAgg                                          >  1:65814/1‑71 (MQ=255)                               gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                            <  1:1713412/52‑1 (MQ=255)                               gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                            <  1:1188368/52‑1 (MQ=255)                               gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                         >  1:2113141/1‑71 (MQ=255)                                cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                         <  1:3682707/70‑1 (MQ=255)                                 tttCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                                                                     >  1:1893355/1‑41 (MQ=255)                                 tttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCg                                                          >  1:3942410/1‑52 (MQ=255)                                  ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCa                                       >  1:2099797/1‑70 (MQ=255)                                  ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                      >  1:2115135/1‑71 (MQ=255)                                  ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                      >  1:1688047/1‑71 (MQ=255)                                   tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                           >  1:2729925/1‑65 (MQ=255)                                   tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc                                     >  1:3463932/1‑71 (MQ=255)                                     aaggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGcc                                    >  1:3397224/3‑70 (MQ=255)                                      cGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                                  <  1:1339931/71‑1 (MQ=255)                                        gCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                >  1:907870/1‑71 (MQ=255)                                         cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                              >  1:1891197/1‑56 (MQ=255)                                         cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                              >  1:3647834/1‑56 (MQ=255)                                         cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                               <  1:2020089/71‑1 (MQ=255)                                          aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                               >  1:3659780/1‑70 (MQ=255)                                           ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCaa                                                      <  1:2031837/46‑1 (MQ=255)                                           ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGtt                             <  1:2367230/71‑1 (MQ=255)                                           gcaaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGCCCGAg                                >  1:1679234/3‑68 (MQ=255)                                            tAATGGCATTACGTTCAATGCACGGGATTTGTTCCTGTCCGGCAACCGGATCGCAGGt                                         <  1:447430/58‑1 (MQ=255)                                            caaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGttg                            <  1:476134/70‑2 (MQ=255)                                              aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                            <  1:1192641/69‑1 (MQ=255)                                              aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                           <  1:2990572/70‑1 (MQ=255)                                               tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                                      <  1:572740/58‑1 (MQ=255)                                               tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                                      <  1:1916719/58‑1 (MQ=255)                                                ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                        >  1:2251416/1‑71 (MQ=255)                                                 gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                               >  1:2302445/1‑63 (MQ=255)                                                   gttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGCGTCACACGTCAg                                      <  1:2645771/53‑1 (MQ=255)                                                   aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                     <  1:2041893/71‑1 (MQ=255)                                                   aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                     <  1:2510351/71‑1 (MQ=255)                                                     tACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtg                   >  1:32290/1‑70 (MQ=255)                                                      aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                 >  1:3553785/1‑56 (MQ=255)                                                      aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                            >  1:3738059/1‑61 (MQ=255)                                                      aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                       >  1:2898859/1‑66 (MQ=255)                                                      aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                  >  1:432397/1‑69 (MQ=255)                                                      aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                  >  1:3945273/1‑69 (MQ=255)                                                       cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                   >  1:54267/1‑53 (MQ=255)                                                       cGTTCAATGCACGGGATATGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                   >  1:935363/1‑53 (MQ=255)                                                        gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgcg                <  1:1594202/71‑1 (MQ=255)                                                        gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                  <  1:1090986/69‑1 (MQ=255)                                                        gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                  <  1:2607773/69‑1 (MQ=255)                                                            aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt             <  1:1713354/70‑1 (MQ=255)                                                            aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt             <  1:1763668/70‑1 (MQ=255)                                                              tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc          <  1:3704535/71‑1 (MQ=255)                                                               gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                      >  1:3031072/1‑58 (MQ=255)                                                               gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                     >  1:1203666/1‑59 (MQ=255)                                                                cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                           >  1:1209205/1‑36 (MQ=255)                                                                cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                            >  1:1182642/1‑51 (MQ=255)                                                                cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc        <  1:482353/71‑1 (MQ=255)                                                                cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc        <  1:327095/71‑1 (MQ=255)                                                                cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccg         <  1:385379/70‑1 (MQ=255)                                                                  cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc           <  1:2370356/66‑1 (MQ=255)                                                                    ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                  >  1:3059344/1‑57 (MQ=255)                                                                      aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt  <  1:755610/71‑1 (MQ=255)                                                                      aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt  >  1:1312345/1‑71 (MQ=255)                                                                      aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTACGCCGCAtt  <  1:3689283/71‑1 (MQ=255)                                                                       tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                              >  1:244553/1‑42 (MQ=255)                                                                       tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc          <  1:188381/62‑1 (MQ=255)                                                                       |                                                                      CGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATT  >  minE/2093614‑2093753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑