Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,093,685	T→C	10.2%	Q389Q (CAA→CAG)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,093,685	0	T	C	10.2%	241.4 / 15.5	89	Q389Q (CAA→CAG)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base T (42/37);  new base C (5/4);  total (48/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

ATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTG  >  minE/2093620‑2093754                                                                  |                                                                      aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                                  >  1:2485069/1‑71 (MQ=255) aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                                  >  1:3745743/1‑71 (MQ=255) aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                                  <  1:6982/71‑1 (MQ=255)   cGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                                >  1:3399835/1‑71 (MQ=255)   cGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                                >  1:168311/1‑71 (MQ=255)   cGCCATCCGCGCGGAGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                                >  1:3222038/1‑71 (MQ=255)    gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                                                               >  1:1567766/1‑71 (MQ=255)      cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                                <  1:101659/68‑1 (MQ=255)      cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCt                                                                <  1:3566136/68‑1 (MQ=255)      cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                             <  1:3664100/71‑1 (MQ=255)      cATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                             <  1:3595496/71‑1 (MQ=255)       aTCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTcc                                                            >  1:1921021/1‑71 (MQ=255)        tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTcc                                                            <  1:3503917/70‑1 (MQ=255)        tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCg                                                           <  1:2604308/71‑1 (MQ=255)          cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                                          >  1:481807/1‑70 (MQ=255)             gcggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                                                                      >  1:1516098/1‑55 (MQ=255)             gcggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCaa                                                       <  1:1269140/70‑1 (MQ=255)               ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg                                                    <  1:1470898/71‑1 (MQ=255)                gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                                   <  1:2104486/71‑1 (MQ=255)                 cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                                   <  1:3166183/70‑1 (MQ=255)                 cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                                   <  1:594042/70‑1 (MQ=255)                 cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGa                                                  >  1:842131/1‑71 (MQ=255)                   tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                                 >  1:2542348/1‑70 (MQ=255)                   tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATc                                                <  1:457663/71‑1 (MQ=255)                   tttACTGCTTTCACGGCATTAATGGAATTACGTTCAATGCAAGGGATTTGTACCTGTCCGGCAACCGGATc                                                >  1:1050312/1‑71 (MQ=255)                     tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                               >  1:2922620/1‑70 (MQ=255)                     tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                               >  1:2917917/1‑70 (MQ=255)                     tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                               >  1:2426003/1‑70 (MQ=255)                     tACTGCTTTCACGGCATTAATGGCATTACGTTCAACGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                               >  1:3823908/1‑70 (MQ=255)                       cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                                  >  1:1282288/1‑49 (MQ=255)                       cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                                  >  1:2207141/1‑49 (MQ=255)                       cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                            >  1:980117/1‑71 (MQ=255)                        tGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAgg                                           >  1:65814/1‑71 (MQ=255)                         gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                             <  1:1713412/52‑1 (MQ=255)                         gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTc                                                             <  1:1188368/52‑1 (MQ=255)                         gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                          >  1:2113141/1‑71 (MQ=255)                          cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                          <  1:3682707/70‑1 (MQ=255)                           tttCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                                                                      >  1:1893355/1‑41 (MQ=255)                           tttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCg                                                           >  1:3942410/1‑52 (MQ=255)                            ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCa                                        >  1:2099797/1‑70 (MQ=255)                            ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                       >  1:2115135/1‑71 (MQ=255)                            ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                       >  1:1688047/1‑71 (MQ=255)                             tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                            >  1:2729925/1‑65 (MQ=255)                             tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc                                      >  1:3463932/1‑71 (MQ=255)                               aaggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGcc                                     >  1:3397224/3‑70 (MQ=255)                                cGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                                   <  1:1339931/71‑1 (MQ=255)                                  gCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                 >  1:907870/1‑71 (MQ=255)                                   cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                               >  1:3647834/1‑56 (MQ=255)                                   cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                               >  1:1891197/1‑56 (MQ=255)                                   cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                <  1:2020089/71‑1 (MQ=255)                                    aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                >  1:3659780/1‑70 (MQ=255)                                     ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCaa                                                       <  1:2031837/46‑1 (MQ=255)                                     ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGtt                              <  1:2367230/71‑1 (MQ=255)                                     gcaaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGCCCGAg                                 >  1:1679234/3‑68 (MQ=255)                                      tAATGGCATTACGTTCAATGCACGGGATTTGTTCCTGTCCGGCAACCGGATCGCAGGt                                          <  1:447430/58‑1 (MQ=255)                                      caaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGttg                             <  1:476134/70‑2 (MQ=255)                                        aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                             <  1:1192641/69‑1 (MQ=255)                                        aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                            <  1:2990572/70‑1 (MQ=255)                                         tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                                       <  1:572740/58‑1 (MQ=255)                                         tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                                       <  1:1916719/58‑1 (MQ=255)                                          ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                         >  1:2251416/1‑71 (MQ=255)                                           gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                >  1:2302445/1‑63 (MQ=255)                                             gttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGCGTCACACGTCAg                                       <  1:2645771/53‑1 (MQ=255)                                             aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                      <  1:2041893/71‑1 (MQ=255)                                             aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                      <  1:2510351/71‑1 (MQ=255)                                               tACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtg                    >  1:32290/1‑70 (MQ=255)                                                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                  >  1:3553785/1‑56 (MQ=255)                                                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                             >  1:3738059/1‑61 (MQ=255)                                                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                        >  1:2898859/1‑66 (MQ=255)                                                aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                   >  1:432397/1‑69 (MQ=255)                                                aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                   >  1:3945273/1‑69 (MQ=255)                                                 cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                    >  1:54267/1‑53 (MQ=255)                                                 cGTTCAATGCACGGGATATGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                    >  1:935363/1‑53 (MQ=255)                                                  gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgcg                 <  1:1594202/71‑1 (MQ=255)                                                  gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                   <  1:1090986/69‑1 (MQ=255)                                                  gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                   <  1:2607773/69‑1 (MQ=255)                                                      aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt              <  1:1713354/70‑1 (MQ=255)                                                      aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt              <  1:1763668/70‑1 (MQ=255)                                                        tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc           <  1:3704535/71‑1 (MQ=255)                                                         gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                       >  1:3031072/1‑58 (MQ=255)                                                         gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                      >  1:1203666/1‑59 (MQ=255)                                                          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                            >  1:1209205/1‑36 (MQ=255)                                                          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                             >  1:1182642/1‑51 (MQ=255)                                                          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc         <  1:327095/71‑1 (MQ=255)                                                          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc         <  1:482353/71‑1 (MQ=255)                                                          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccg          <  1:385379/70‑1 (MQ=255)                                                            cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc            <  1:2370356/66‑1 (MQ=255)                                                              ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                   >  1:3059344/1‑57 (MQ=255)                                                                aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt   <  1:755610/71‑1 (MQ=255)                                                                aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt   >  1:1312345/1‑71 (MQ=255)                                                                aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTACGCCGCAtt   <  1:3689283/71‑1 (MQ=255)                                                                 tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                               >  1:244553/1‑42 (MQ=255)                                                                 tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc           <  1:188381/62‑1 (MQ=255)                                                                  ttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg  <  1:2937035/70‑1 (MQ=255)                                                                  |                                                                      ATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTG  >  minE/2093620‑2093754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑