Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,093,700	A→G	14.8%	V384V (GTT→GTC)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,093,700	0	A	G	14.8%	215.8 / 26.2	88	V384V (GTT→GTC)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base A (37/38);  new base G (5/8);  total (42/46)
Fisher's exact test for biased strand distribution p-value = 5.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAC  >  minE/2093632‑2093770                                                                     |                                                                       gcggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCaa                                                                       <  1:1269140/70‑1 (MQ=255)   ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg                                                                    <  1:1470898/71‑1 (MQ=255)    gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                                                   <  1:2104486/71‑1 (MQ=255)     cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                                                   <  1:3166183/70‑1 (MQ=255)     cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                                                   <  1:594042/70‑1 (MQ=255)     cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGa                                                                  >  1:842131/1‑71 (MQ=255)       tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                                                 >  1:2542348/1‑70 (MQ=255)       tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATc                                                                <  1:457663/71‑1 (MQ=255)       tttACTGCTTTCACGGCATTAATGGAATTACGTTCAATGCAAGGGATTTGTACCTGTCCGGCAACCGGATc                                                                >  1:1050312/1‑71 (MQ=255)         tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                               >  1:2922620/1‑70 (MQ=255)         tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                               >  1:2917917/1‑70 (MQ=255)         tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                               >  1:2426003/1‑70 (MQ=255)         tACTGCTTTCACGGCATTAATGGCATTACGTTCAACGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                               >  1:3823908/1‑70 (MQ=255)           cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                            >  1:980117/1‑71 (MQ=255)            tGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAgg                                                           >  1:65814/1‑71 (MQ=255)             gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                                          >  1:2113141/1‑71 (MQ=255)              cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                                          <  1:3682707/70‑1 (MQ=255)                ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCa                                                        >  1:2099797/1‑70 (MQ=255)                ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                                       >  1:1688047/1‑71 (MQ=255)                ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                                       >  1:2115135/1‑71 (MQ=255)                 tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                            >  1:2729925/1‑65 (MQ=255)                 tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc                                                      >  1:3463932/1‑71 (MQ=255)                   aaggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGcc                                                     >  1:3397224/3‑70 (MQ=255)                    cGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                                                   <  1:1339931/71‑1 (MQ=255)                      gCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                                 >  1:907870/1‑71 (MQ=255)                       cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                               >  1:3647834/1‑56 (MQ=255)                       cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                               >  1:1891197/1‑56 (MQ=255)                       cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                <  1:2020089/71‑1 (MQ=255)                        aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                >  1:3659780/1‑70 (MQ=255)                         ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCaa                                                                       <  1:2031837/46‑1 (MQ=255)                         ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGtt                                              <  1:2367230/71‑1 (MQ=255)                         gcaaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGCCCGAg                                                 >  1:1679234/3‑68 (MQ=255)                          tAATGGCATTACGTTCAATGCACGGGATTTGTTCCTGTCCGGCAACCGGATCGCAGGt                                                          <  1:447430/58‑1 (MQ=255)                          caaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGttg                                             <  1:476134/70‑2 (MQ=255)                            aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                             <  1:1192641/69‑1 (MQ=255)                            aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                            <  1:2990572/70‑1 (MQ=255)                             tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                                                       <  1:572740/58‑1 (MQ=255)                             tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                                                       <  1:1916719/58‑1 (MQ=255)                              ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                                         >  1:2251416/1‑71 (MQ=255)                               gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                >  1:2302445/1‑63 (MQ=255)                                 gttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGCGTCACACGTCAg                                                       <  1:2645771/53‑1 (MQ=255)                                 aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                      <  1:2510351/71‑1 (MQ=255)                                 aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                      <  1:2041893/71‑1 (MQ=255)                                   tACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtg                                    >  1:32290/1‑70 (MQ=255)                                    aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                  >  1:3553785/1‑56 (MQ=255)                                    aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                             >  1:3738059/1‑61 (MQ=255)                                    aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                                        >  1:2898859/1‑66 (MQ=255)                                    aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                   >  1:432397/1‑69 (MQ=255)                                    aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                   >  1:3945273/1‑69 (MQ=255)                                     cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                                    >  1:54267/1‑53 (MQ=255)                                     cGTTCAATGCACGGGATATGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                                    >  1:935363/1‑53 (MQ=255)                                      gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgcg                                 <  1:1594202/71‑1 (MQ=255)                                      gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                   <  1:1090986/69‑1 (MQ=255)                                      gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                   <  1:2607773/69‑1 (MQ=255)                                          aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                              <  1:1713354/70‑1 (MQ=255)                                          aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                              <  1:1763668/70‑1 (MQ=255)                                            tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                           <  1:3704535/71‑1 (MQ=255)                                             gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                       >  1:3031072/1‑58 (MQ=255)                                             gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                      >  1:1203666/1‑59 (MQ=255)                                              cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                            >  1:1209205/1‑36 (MQ=255)                                              cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                             >  1:1182642/1‑51 (MQ=255)                                              cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                         <  1:482353/71‑1 (MQ=255)                                              cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                         <  1:327095/71‑1 (MQ=255)                                              cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccg                          <  1:385379/70‑1 (MQ=255)                                                cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                            <  1:2370356/66‑1 (MQ=255)                                                  ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                   >  1:3059344/1‑57 (MQ=255)                                                    aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                   >  1:1312345/1‑71 (MQ=255)                                                    aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                   <  1:755610/71‑1 (MQ=255)                                                    aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTACGCCGCAtt                   <  1:3689283/71‑1 (MQ=255)                                                     tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                               >  1:244553/1‑42 (MQ=255)                                                     tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                           <  1:188381/62‑1 (MQ=255)                                                      ttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                  <  1:2937035/70‑1 (MQ=255)                                                       tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                             >  1:2403525/1‑42 (MQ=255)                                                       tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                             >  1:1404608/1‑42 (MQ=255)                                                       tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                  <  1:2619489/69‑1 (MQ=255)                                                        gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                  >  1:1220318/1‑52 (MQ=255)                                                         tACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCac               <  1:2748418/70‑2 (MQ=255)                                                         tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt               <  1:1846827/70‑1 (MQ=255)                                                         tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt               <  1:665244/70‑1 (MQ=255)                                                         tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt               <  1:660514/70‑1 (MQ=255)                                                           ccTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACAcc            <  1:621551/71‑1 (MQ=255)                                                           ccTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACAcc            <  1:3773520/71‑1 (MQ=255)                                                            cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCt           <  1:3419973/71‑1 (MQ=255)                                                            cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCt           <  1:3625457/71‑1 (MQ=255)                                                               tCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTg          <  1:3655475/69‑1 (MQ=9)                                                                 cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgcgc       <  1:2887763/70‑1 (MQ=255)                                                                   gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                  <  1:1494542/41‑1 (MQ=255)                                                                   gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                  <  1:212616/41‑1 (MQ=255)                                                                   gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCAATCGCGATTTCCGCCGCATTGCATACCTGCGCCg     >  1:3141530/1‑70 (MQ=255)                                                                    cAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                   <  1:1213022/39‑1 (MQ=255)                                                                     aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc  >  1:513882/1‑71 (MQ=255)                                                                     aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc  <  1:1732695/71‑1 (MQ=255)                                                                     aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc  <  1:3014455/71‑1 (MQ=255)                                                                     |                                                                       GCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAC  >  minE/2093632‑2093770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑