Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,093,706	A→G	17.0%	D382D (GAT→GAC)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,093,706	0	A	G	17.0%	214.7 / 34.9	94	D382D (GAT→GAC)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base A (41/37);  new base G (6/10);  total (47/47)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

CGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGC  >  minE/2093636‑2093773                                                                       |                                                                    cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGa                                                                     >  1:842131/1‑71 (MQ=255)   tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                                                    >  1:2542348/1‑70 (MQ=255)   tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATc                                                                   <  1:457663/71‑1 (MQ=255)   tttACTGCTTTCACGGCATTAATGGAATTACGTTCAATGCAAGGGATTTGTACCTGTCCGGCAACCGGATc                                                                   >  1:1050312/1‑71 (MQ=255)     tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                                  >  1:2426003/1‑70 (MQ=255)     tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                                  >  1:2917917/1‑70 (MQ=255)     tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                                  >  1:2922620/1‑70 (MQ=255)     tACTGCTTTCACGGCATTAATGGCATTACGTTCAACGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                                  >  1:3823908/1‑70 (MQ=255)       cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                               >  1:980117/1‑71 (MQ=255)        tGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAgg                                                              >  1:65814/1‑71 (MQ=255)         gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                                             >  1:2113141/1‑71 (MQ=255)          cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                                             <  1:3682707/70‑1 (MQ=255)            ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCa                                                           >  1:2099797/1‑70 (MQ=255)            ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                                          >  1:2115135/1‑71 (MQ=255)            ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                                          >  1:1688047/1‑71 (MQ=255)             tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                               >  1:2729925/1‑65 (MQ=255)             tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc                                                         >  1:3463932/1‑71 (MQ=255)               aaggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGcc                                                        >  1:3397224/3‑70 (MQ=255)                cGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                                                      <  1:1339931/71‑1 (MQ=255)                  gCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                                    >  1:907870/1‑71 (MQ=255)                   cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                                  >  1:3647834/1‑56 (MQ=255)                   cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                                  >  1:1891197/1‑56 (MQ=255)                   cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                   <  1:2020089/71‑1 (MQ=255)                    aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                   >  1:3659780/1‑70 (MQ=255)                     ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGtt                                                 <  1:2367230/71‑1 (MQ=255)                     gcaaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGCCCGAg                                                    >  1:1679234/3‑68 (MQ=255)                      tAATGGCATTACGTTCAATGCACGGGATTTGTTCCTGTCCGGCAACCGGATCGCAGGt                                                             <  1:447430/58‑1 (MQ=255)                      caaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGttg                                                <  1:476134/70‑2 (MQ=255)                        aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                <  1:1192641/69‑1 (MQ=255)                        aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                               <  1:2990572/70‑1 (MQ=255)                         tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                                                          <  1:1916719/58‑1 (MQ=255)                         tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                                                          <  1:572740/58‑1 (MQ=255)                          ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                                            >  1:2251416/1‑71 (MQ=255)                           gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                   >  1:2302445/1‑63 (MQ=255)                             gttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGCGTCACACGTCAg                                                          <  1:2645771/53‑1 (MQ=255)                             aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                         <  1:2510351/71‑1 (MQ=255)                             aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                         <  1:2041893/71‑1 (MQ=255)                               tACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtg                                       >  1:32290/1‑70 (MQ=255)                                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                     >  1:3553785/1‑56 (MQ=255)                                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                >  1:3738059/1‑61 (MQ=255)                                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                                           >  1:2898859/1‑66 (MQ=255)                                aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                      >  1:3945273/1‑69 (MQ=255)                                aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                      >  1:432397/1‑69 (MQ=255)                                 cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                                       >  1:54267/1‑53 (MQ=255)                                 cGTTCAATGCACGGGATATGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                                       >  1:935363/1‑53 (MQ=255)                                  gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgcg                                    <  1:1594202/71‑1 (MQ=255)                                  gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                      <  1:2607773/69‑1 (MQ=255)                                  gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                      <  1:1090986/69‑1 (MQ=255)                                      aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                 <  1:1713354/70‑1 (MQ=255)                                      aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                 <  1:1763668/70‑1 (MQ=255)                                        tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                              <  1:3704535/71‑1 (MQ=255)                                         gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                          >  1:3031072/1‑58 (MQ=255)                                         gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                         >  1:1203666/1‑59 (MQ=255)                                          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                               >  1:1209205/1‑36 (MQ=255)                                          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                >  1:1182642/1‑51 (MQ=255)                                          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                            <  1:482353/71‑1 (MQ=255)                                          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                            <  1:327095/71‑1 (MQ=255)                                          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccg                             <  1:385379/70‑1 (MQ=255)                                            cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                               <  1:2370356/66‑1 (MQ=255)                                              ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                      >  1:3059344/1‑57 (MQ=255)                                                aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                      >  1:1312345/1‑71 (MQ=255)                                                aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                      <  1:755610/71‑1 (MQ=255)                                                aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTACGCCGCAtt                      <  1:3689283/71‑1 (MQ=255)                                                 tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                  >  1:244553/1‑42 (MQ=255)                                                 tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                              <  1:188381/62‑1 (MQ=255)                                                  ttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                     <  1:2937035/70‑1 (MQ=255)                                                   tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                >  1:1404608/1‑42 (MQ=255)                                                   tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                >  1:2403525/1‑42 (MQ=255)                                                   tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                     <  1:2619489/69‑1 (MQ=255)                                                    gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                     >  1:1220318/1‑52 (MQ=255)                                                     tACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCac                  <  1:2748418/70‑2 (MQ=255)                                                     tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                  <  1:1846827/70‑1 (MQ=255)                                                     tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                  <  1:665244/70‑1 (MQ=255)                                                     tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                  <  1:660514/70‑1 (MQ=255)                                                       ccTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACAcc               <  1:621551/71‑1 (MQ=255)                                                       ccTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACAcc               <  1:3773520/71‑1 (MQ=255)                                                        cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCt              <  1:3625457/71‑1 (MQ=255)                                                        cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCt              <  1:3419973/71‑1 (MQ=255)                                                           tCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTg             <  1:3655475/69‑1 (MQ=9)                                                             cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgcgc          <  1:2887763/70‑1 (MQ=255)                                                               gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                     <  1:1494542/41‑1 (MQ=255)                                                               gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                     <  1:212616/41‑1 (MQ=255)                                                               gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCAATCGCGATTTCCGCCGCATTGCATACCTGCGCCg        >  1:3141530/1‑70 (MQ=255)                                                                cgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCggg      <  1:1670782/69‑2 (MQ=255)                                                                cgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCggg      <  1:2687725/69‑2 (MQ=255)                                                                cAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                      <  1:1213022/39‑1 (MQ=255)                                                                 aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc     <  1:3014455/71‑1 (MQ=255)                                                                 aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc     >  1:513882/1‑71 (MQ=255)                                                                 aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc     <  1:1732695/71‑1 (MQ=255)                                                                  aCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACt    >  1:2419385/1‑71 (MQ=255)                                                                  aCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACt    >  1:1686890/1‑71 (MQ=255)                                                                   ccGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCGGGCtt   >  1:2433961/1‑70 (MQ=255)                                                                   ccGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAt                                  <  1:610524/40‑1 (MQ=255)                                                                    cGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTgc  >  1:613875/1‑71 (MQ=255)                                                                      gATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                 >  1:556891/1‑38 (MQ=255)                                                                       aTCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                              <  1:3048837/40‑1 (MQ=255)                                                                       |                                                                    CGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGC  >  minE/2093636‑2093773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑