Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,093,709	G→A	18.6%	C381C (TGC→TGT)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,093,709	0	G	A	18.6%	194.0 / 33.4	86	C381C (TGC→TGT)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base G (33/37);  new base A (6/10);  total (39/47)
Fisher's exact test for biased strand distribution p-value = 5.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.88e-01

TACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCC  >  minE/2093640‑2093778                                                                      |                                                                      tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                                       >  1:2426003/1‑70 (MQ=255) tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                                       >  1:2917917/1‑70 (MQ=255) tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                                       >  1:2922620/1‑70 (MQ=255) tACTGCTTTCACGGCATTAATGGCATTACGTTCAACGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                                       >  1:3823908/1‑70 (MQ=255)   cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                                    >  1:980117/1‑71 (MQ=255)    tGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAgg                                                                   >  1:65814/1‑71 (MQ=255)     gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                                                  >  1:2113141/1‑71 (MQ=255)      cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                                                  <  1:3682707/70‑1 (MQ=255)        ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCa                                                                >  1:2099797/1‑70 (MQ=255)        ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                                               >  1:2115135/1‑71 (MQ=255)        ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                                               >  1:1688047/1‑71 (MQ=255)         tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                                    >  1:2729925/1‑65 (MQ=255)         tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc                                                              >  1:3463932/1‑71 (MQ=255)           aaggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGcc                                                             >  1:3397224/3‑70 (MQ=255)            cGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                                                           <  1:1339931/71‑1 (MQ=255)              gCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                                         >  1:907870/1‑71 (MQ=255)               cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                                       >  1:3647834/1‑56 (MQ=255)               cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                                       >  1:1891197/1‑56 (MQ=255)               cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                        <  1:2020089/71‑1 (MQ=255)                aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                        >  1:3659780/1‑70 (MQ=255)                 ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGtt                                                      <  1:2367230/71‑1 (MQ=255)                 gcaaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGCCCGAg                                                         >  1:1679234/3‑68 (MQ=255)                  tAATGGCATTACGTTCAATGCACGGGATTTGTTCCTGTCCGGCAACCGGATCGCAGGt                                                                  <  1:447430/58‑1 (MQ=255)                  caaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGttg                                                     <  1:476134/70‑2 (MQ=255)                    aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                     <  1:1192641/69‑1 (MQ=255)                    aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                    <  1:2990572/70‑1 (MQ=255)                     tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                                                               <  1:1916719/58‑1 (MQ=255)                     tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                                                               <  1:572740/58‑1 (MQ=255)                      ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                                                 >  1:2251416/1‑71 (MQ=255)                       gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                        >  1:2302445/1‑63 (MQ=255)                         gttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGCGTCACACGTCAg                                                               <  1:2645771/53‑1 (MQ=255)                         aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                              <  1:2510351/71‑1 (MQ=255)                         aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                              <  1:2041893/71‑1 (MQ=255)                           tACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtg                                            >  1:32290/1‑70 (MQ=255)                            aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                          >  1:3553785/1‑56 (MQ=255)                            aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                     >  1:3738059/1‑61 (MQ=255)                            aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                                                >  1:2898859/1‑66 (MQ=255)                            aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                           >  1:432397/1‑69 (MQ=255)                            aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                           >  1:3945273/1‑69 (MQ=255)                             cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                                            >  1:54267/1‑53 (MQ=255)                             cGTTCAATGCACGGGATATGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                                            >  1:935363/1‑53 (MQ=255)                              gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgcg                                         <  1:1594202/71‑1 (MQ=255)                              gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                           <  1:2607773/69‑1 (MQ=255)                              gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                           <  1:1090986/69‑1 (MQ=255)                                  aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                      <  1:1763668/70‑1 (MQ=255)                                  aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                      <  1:1713354/70‑1 (MQ=255)                                    tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                   <  1:3704535/71‑1 (MQ=255)                                     gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                               >  1:3031072/1‑58 (MQ=255)                                     gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                              >  1:1203666/1‑59 (MQ=255)                                      cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                                    >  1:1209205/1‑36 (MQ=255)                                      cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                     >  1:1182642/1‑51 (MQ=255)                                      cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                 <  1:482353/71‑1 (MQ=255)                                      cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                 <  1:327095/71‑1 (MQ=255)                                      cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccg                                  <  1:385379/70‑1 (MQ=255)                                        cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                                    <  1:2370356/66‑1 (MQ=255)                                          ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                           >  1:3059344/1‑57 (MQ=255)                                            aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                           <  1:755610/71‑1 (MQ=255)                                            aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                           >  1:1312345/1‑71 (MQ=255)                                            aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTACGCCGCAtt                           <  1:3689283/71‑1 (MQ=255)                                             tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                       >  1:244553/1‑42 (MQ=255)                                             tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                   <  1:188381/62‑1 (MQ=255)                                              ttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                          <  1:2937035/70‑1 (MQ=255)                                               tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                     >  1:2403525/1‑42 (MQ=255)                                               tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                     >  1:1404608/1‑42 (MQ=255)                                               tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                          <  1:2619489/69‑1 (MQ=255)                                                gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                          >  1:1220318/1‑52 (MQ=255)                                                 tACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCac                       <  1:2748418/70‑2 (MQ=255)                                                 tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                       <  1:660514/70‑1 (MQ=255)                                                 tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                       <  1:665244/70‑1 (MQ=255)                                                 tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                       <  1:1846827/70‑1 (MQ=255)                                                   ccTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACAcc                    <  1:621551/71‑1 (MQ=255)                                                   ccTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACAcc                    <  1:3773520/71‑1 (MQ=255)                                                    cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCt                   <  1:3625457/71‑1 (MQ=255)                                                    cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCt                   <  1:3419973/71‑1 (MQ=255)                                                       tCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTg                  <  1:3655475/69‑1 (MQ=9)                                                         cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgcgc               <  1:2887763/70‑1 (MQ=255)                                                           gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                          <  1:1494542/41‑1 (MQ=255)                                                           gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                          <  1:212616/41‑1 (MQ=255)                                                           gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCAATCGCGATTTCCGCCGCATTGCATACCTGCGCCg             >  1:3141530/1‑70 (MQ=255)                                                            cgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCggg           <  1:1670782/69‑2 (MQ=255)                                                            cgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCggg           <  1:2687725/69‑2 (MQ=255)                                                            cAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                           <  1:1213022/39‑1 (MQ=255)                                                             aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc          >  1:513882/1‑71 (MQ=255)                                                             aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc          <  1:1732695/71‑1 (MQ=255)                                                             aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc          <  1:3014455/71‑1 (MQ=255)                                                              aCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACt         >  1:1686890/1‑71 (MQ=255)                                                              aCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACt         >  1:2419385/1‑71 (MQ=255)                                                               ccGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCGGGCtt        >  1:2433961/1‑70 (MQ=255)                                                               ccGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAt                                       <  1:610524/40‑1 (MQ=255)                                                                cGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTgc       >  1:613875/1‑71 (MQ=255)                                                                  gATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                      >  1:556891/1‑38 (MQ=255)                                                                   aTCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                   <  1:3048837/40‑1 (MQ=255)                                                                      gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGccc  <  1:838594/70‑1 (MQ=255)                                                                      |                                                                      TACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCC  >  minE/2093640‑2093778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑