Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,093,712	G→C	19.0%	T380T (ACC→ACG)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,093,712	0	G	C	19.0%	183.5 / 35.1	84	T380T (ACC→ACG)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base G (30/38);  new base C (6/10);  total (36/48)
Fisher's exact test for biased strand distribution p-value = 7.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

CTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAG  >  minE/2093642‑2093780                                                                       |                                                                     cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                                      >  1:980117/1‑71 (MQ=255)  tGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAgg                                                                     >  1:65814/1‑71 (MQ=255)   gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                                                    >  1:2113141/1‑71 (MQ=255)    cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                                                    <  1:3682707/70‑1 (MQ=255)      ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCa                                                                  >  1:2099797/1‑70 (MQ=255)      ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                                                 >  1:2115135/1‑71 (MQ=255)      ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                                                 >  1:1688047/1‑71 (MQ=255)       tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                                      >  1:2729925/1‑65 (MQ=255)       tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc                                                                >  1:3463932/1‑71 (MQ=255)         aaggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGcc                                                               >  1:3397224/3‑70 (MQ=255)          cGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                                                             <  1:1339931/71‑1 (MQ=255)            gCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                                           >  1:907870/1‑71 (MQ=255)             cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                          <  1:2020089/71‑1 (MQ=255)              aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                          >  1:3659780/1‑70 (MQ=255)               ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGtt                                                        <  1:2367230/71‑1 (MQ=255)               gcaaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGCCCGAg                                                           >  1:1679234/3‑68 (MQ=255)                tAATGGCATTACGTTCAATGCACGGGATTTGTTCCTGTCCGGCAACCGGATCGCAGGt                                                                    <  1:447430/58‑1 (MQ=255)                caaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGttg                                                       <  1:476134/70‑2 (MQ=255)                  aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                       <  1:1192641/69‑1 (MQ=255)                  aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                      <  1:2990572/70‑1 (MQ=255)                   tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                                                                 <  1:1916719/58‑1 (MQ=255)                   tGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                                                                 <  1:572740/58‑1 (MQ=255)                    ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                                                   >  1:2251416/1‑71 (MQ=255)                     gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                          >  1:2302445/1‑63 (MQ=255)                       gttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGCGTCACACGTCAg                                                                 <  1:2645771/53‑1 (MQ=255)                       aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                                <  1:2510351/71‑1 (MQ=255)                       aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                                <  1:2041893/71‑1 (MQ=255)                         tACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtg                                              >  1:32290/1‑70 (MQ=255)                          aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                            >  1:3553785/1‑56 (MQ=255)                          aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                       >  1:3738059/1‑61 (MQ=255)                          aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                                                  >  1:2898859/1‑66 (MQ=255)                          aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                             >  1:3945273/1‑69 (MQ=255)                          aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                             >  1:432397/1‑69 (MQ=255)                           cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                                              >  1:54267/1‑53 (MQ=255)                           cGTTCAATGCACGGGATATGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                                              >  1:935363/1‑53 (MQ=255)                            gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgcg                                           <  1:1594202/71‑1 (MQ=255)                            gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                             <  1:2607773/69‑1 (MQ=255)                            gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                             <  1:1090986/69‑1 (MQ=255)                                aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                        <  1:1763668/70‑1 (MQ=255)                                aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                        <  1:1713354/70‑1 (MQ=255)                                  tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                     <  1:3704535/71‑1 (MQ=255)                                   gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                                 >  1:3031072/1‑58 (MQ=255)                                   gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                                >  1:1203666/1‑59 (MQ=255)                                    cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                                      >  1:1209205/1‑36 (MQ=255)                                    cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                       >  1:1182642/1‑51 (MQ=255)                                    cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                   <  1:482353/71‑1 (MQ=255)                                    cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                   <  1:327095/71‑1 (MQ=255)                                    cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccg                                    <  1:385379/70‑1 (MQ=255)                                      cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                                      <  1:2370356/66‑1 (MQ=255)                                        ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                             >  1:3059344/1‑57 (MQ=255)                                          aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                             >  1:1312345/1‑71 (MQ=255)                                          aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                             <  1:755610/71‑1 (MQ=255)                                          aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTACGCCGCAtt                             <  1:3689283/71‑1 (MQ=255)                                           tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                         >  1:244553/1‑42 (MQ=255)                                           tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                     <  1:188381/62‑1 (MQ=255)                                            ttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                            <  1:2937035/70‑1 (MQ=255)                                             tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                       >  1:1404608/1‑42 (MQ=255)                                             tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                       >  1:2403525/1‑42 (MQ=255)                                             tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                            <  1:2619489/69‑1 (MQ=255)                                              gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                            >  1:1220318/1‑52 (MQ=255)                                               tACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCac                         <  1:2748418/70‑2 (MQ=255)                                               tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                         <  1:660514/70‑1 (MQ=255)                                               tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                         <  1:665244/70‑1 (MQ=255)                                               tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                         <  1:1846827/70‑1 (MQ=255)                                                 ccTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACAcc                      <  1:3773520/71‑1 (MQ=255)                                                 ccTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACAcc                      <  1:621551/71‑1 (MQ=255)                                                  cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCt                     <  1:3419973/71‑1 (MQ=255)                                                  cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCt                     <  1:3625457/71‑1 (MQ=255)                                                     tCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTg                    <  1:3655475/69‑1 (MQ=9)                                                       cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgcgc                 <  1:2887763/70‑1 (MQ=255)                                                         gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                            <  1:212616/41‑1 (MQ=255)                                                         gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                            <  1:1494542/41‑1 (MQ=255)                                                         gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCAATCGCGATTTCCGCCGCATTGCATACCTGCGCCg               >  1:3141530/1‑70 (MQ=255)                                                          cgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCggg             <  1:1670782/69‑2 (MQ=255)                                                          cgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCggg             <  1:2687725/69‑2 (MQ=255)                                                          cAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                             <  1:1213022/39‑1 (MQ=255)                                                           aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc            <  1:3014455/71‑1 (MQ=255)                                                           aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc            >  1:513882/1‑71 (MQ=255)                                                           aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc            <  1:1732695/71‑1 (MQ=255)                                                            aCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACt           >  1:1686890/1‑71 (MQ=255)                                                            aCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACt           >  1:2419385/1‑71 (MQ=255)                                                             ccGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCGGGCtt          >  1:2433961/1‑70 (MQ=255)                                                             ccGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAt                                         <  1:610524/40‑1 (MQ=255)                                                              cGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTgc         >  1:613875/1‑71 (MQ=255)                                                                gATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                        >  1:556891/1‑38 (MQ=255)                                                                 aTCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                     <  1:3048837/40‑1 (MQ=255)                                                                    gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGccc    <  1:838594/70‑1 (MQ=255)                                                                     cAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCag  >  1:2013484/1‑71 (MQ=255)                                                                       |                                                                     CTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAG  >  minE/2093642‑2093780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑