Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,093,718 C→A 19.2% G378G (GGG→GGT)  tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,093,7180CA19.2% 206.1 / 25.8 78G378G (GGG→GGT) tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base C (24/39);  new base A (8/7);  total (32/46)
Fisher's exact test for biased strand distribution p-value = 3.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

TCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCA  >  minE/2093648‑2093787
                                                                      |                                                                     
tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc                                                                       >  1:3463932/1‑71 (MQ=255)
  aaggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGcc                                                                      >  1:3397224/3‑70 (MQ=255)
   cGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                                                                    <  1:1339931/71‑1 (MQ=255)
     gCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                                                  >  1:907870/1‑71 (MQ=255)
      cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                                 <  1:2020089/71‑1 (MQ=255)
       aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                                 >  1:3659780/1‑70 (MQ=255)
        ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGtt                                                               <  1:2367230/71‑1 (MQ=255)
        gcaaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGCCCGAg                                                                  >  1:1679234/3‑68 (MQ=255)
         caaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGttg                                                              <  1:476134/70‑2 (MQ=255)
           aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                              <  1:1192641/69‑1 (MQ=255)
           aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                             <  1:2990572/70‑1 (MQ=255)
             ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                                                          >  1:2251416/1‑71 (MQ=255)
              gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                                 >  1:2302445/1‑63 (MQ=255)
                aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                                       <  1:2041893/71‑1 (MQ=255)
                aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                                       <  1:2510351/71‑1 (MQ=255)
                  tACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtg                                                     >  1:32290/1‑70 (MQ=255)
                   aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                                   >  1:3553785/1‑56 (MQ=255)
                   aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                              >  1:3738059/1‑61 (MQ=255)
                   aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                                                         >  1:2898859/1‑66 (MQ=255)
                   aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                                    >  1:3945273/1‑69 (MQ=255)
                   aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                                    >  1:432397/1‑69 (MQ=255)
                    cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                                                     >  1:54267/1‑53 (MQ=255)
                    cGTTCAATGCACGGGATATGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                                                     >  1:935363/1‑53 (MQ=255)
                     gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgcg                                                  <  1:1594202/71‑1 (MQ=255)
                     gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                    <  1:2607773/69‑1 (MQ=255)
                     gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                    <  1:1090986/69‑1 (MQ=255)
                         aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                               <  1:1713354/70‑1 (MQ=255)
                         aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                               <  1:1763668/70‑1 (MQ=255)
                           tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                            <  1:3704535/71‑1 (MQ=255)
                            gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                                        >  1:3031072/1‑58 (MQ=255)
                            gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                                       >  1:1203666/1‑59 (MQ=255)
                             cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                              >  1:1182642/1‑51 (MQ=255)
                             cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                          <  1:327095/71‑1 (MQ=255)
                             cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                          <  1:482353/71‑1 (MQ=255)
                             cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccg                                           <  1:385379/70‑1 (MQ=255)
                               cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                                             <  1:2370356/66‑1 (MQ=255)
                                 ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                    >  1:3059344/1‑57 (MQ=255)
                                   aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                    <  1:755610/71‑1 (MQ=255)
                                   aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                    >  1:1312345/1‑71 (MQ=255)
                                   aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTACGCCGCAtt                                    <  1:3689283/71‑1 (MQ=255)
                                    tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                                >  1:244553/1‑42 (MQ=255)
                                    tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                            <  1:188381/62‑1 (MQ=255)
                                     ttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                   <  1:2937035/70‑1 (MQ=255)
                                      tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                              >  1:1404608/1‑42 (MQ=255)
                                      tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                              >  1:2403525/1‑42 (MQ=255)
                                      tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                   <  1:2619489/69‑1 (MQ=255)
                                       gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                                   >  1:1220318/1‑52 (MQ=255)
                                        tACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCac                                <  1:2748418/70‑2 (MQ=255)
                                        tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                <  1:660514/70‑1 (MQ=255)
                                        tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                <  1:665244/70‑1 (MQ=255)
                                        tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                <  1:1846827/70‑1 (MQ=255)
                                          ccTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACAcc                             <  1:621551/71‑1 (MQ=255)
                                          ccTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACAcc                             <  1:3773520/71‑1 (MQ=255)
                                           cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCt                            <  1:3419973/71‑1 (MQ=255)
                                           cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCt                            <  1:3625457/71‑1 (MQ=255)
                                              tCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTg                           <  1:3655475/69‑1 (MQ=9)
                                                cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgcgc                        <  1:2887763/70‑1 (MQ=255)
                                                  gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                                   <  1:212616/41‑1 (MQ=255)
                                                  gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                                   <  1:1494542/41‑1 (MQ=255)
                                                  gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCAATCGCGATTTCCGCCGCATTGCATACCTGCGCCg                      >  1:3141530/1‑70 (MQ=255)
                                                   cgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCggg                    <  1:1670782/69‑2 (MQ=255)
                                                   cgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCggg                    <  1:2687725/69‑2 (MQ=255)
                                                   cAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                    <  1:1213022/39‑1 (MQ=255)
                                                    aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc                   >  1:513882/1‑71 (MQ=255)
                                                    aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc                   <  1:3014455/71‑1 (MQ=255)
                                                    aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc                   <  1:1732695/71‑1 (MQ=255)
                                                     aCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACt                  >  1:1686890/1‑71 (MQ=255)
                                                     aCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACt                  >  1:2419385/1‑71 (MQ=255)
                                                      ccGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCGGGCtt                 >  1:2433961/1‑70 (MQ=255)
                                                      ccGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAt                                                <  1:610524/40‑1 (MQ=255)
                                                       cGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTgc                >  1:613875/1‑71 (MQ=255)
                                                         gATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                               >  1:556891/1‑38 (MQ=255)
                                                          aTCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                            <  1:3048837/40‑1 (MQ=255)
                                                             gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGccc           <  1:838594/70‑1 (MQ=255)
                                                              cAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCag         >  1:2013484/1‑71 (MQ=255)
                                                                 gTCAGACCGAGGTTGTGCTCCATGGCGATTTccgcc                                         >  1:2021829/1‑36 (MQ=19)
                                                                 gTCAGACCGAGGTTGTGCTCCATGGCGATTTccgcc                                         >  1:179941/1‑36 (MQ=19)
                                                                 gTCAGACCGAGGTTGTGCTCCATGGCGATTTccgcc                                         >  1:748225/1‑36 (MQ=19)
                                                                  tCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtagt     <  1:103575/71‑1 (MQ=255)
                                                                    aGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTTCATACCTGCGCCGGACTGCCGCCCAGTAGTTc   <  1:1713055/71‑1 (MQ=255)
                                                                    aGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt    >  1:328614/1‑70 (MQ=255)
                                                                     gCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCa  <  1:1277156/71‑1 (MQ=255)
                                                                      cccAAGGTTATGGTCCATCGCGATTTCCGCCGCATTGCATACCTgcgc                        >  1:2891604/1‑48 (MQ=255)
                                                                      |                                                                     
TCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCA  >  minE/2093648‑2093787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑