Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,093,721 A→G 20.0% L377L (CTT→CTC)  tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,093,7210AG20.0% 168.4 / 36.4 80L377L (CTT→CTC) tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base A (24/40);  new base G (9/7);  total (33/47)
Fisher's exact test for biased strand distribution p-value = 2.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

CGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTT  >  minE/2093651‑2093790
                                                                      |                                                                     
cGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                                                                       <  1:1339931/71‑1 (MQ=255)
  gCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                                                     >  1:907870/1‑71 (MQ=255)
   cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                                    <  1:2020089/71‑1 (MQ=255)
    aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                                    >  1:3659780/1‑70 (MQ=255)
     ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGtt                                                                  <  1:2367230/71‑1 (MQ=255)
     gcaaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGCCCGAg                                                                     >  1:1679234/3‑68 (MQ=255)
      caaTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGttg                                                                 <  1:476134/70‑2 (MQ=255)
        aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                 <  1:1192641/69‑1 (MQ=255)
        aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                                <  1:2990572/70‑1 (MQ=255)
          ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                                                             >  1:2251416/1‑71 (MQ=255)
           gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                                    >  1:2302445/1‑63 (MQ=255)
             aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                                          <  1:2041893/71‑1 (MQ=255)
             aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                                          <  1:2510351/71‑1 (MQ=255)
               tACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtg                                                        >  1:32290/1‑70 (MQ=255)
                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                                      >  1:3553785/1‑56 (MQ=255)
                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                 >  1:3738059/1‑61 (MQ=255)
                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                                                            >  1:2898859/1‑66 (MQ=255)
                aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                                       >  1:432397/1‑69 (MQ=255)
                aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                                       >  1:3945273/1‑69 (MQ=255)
                  gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgcg                                                     <  1:1594202/71‑1 (MQ=255)
                  gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                       <  1:2607773/69‑1 (MQ=255)
                  gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                       <  1:1090986/69‑1 (MQ=255)
                      aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                                  <  1:1763668/70‑1 (MQ=255)
                      aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                                  <  1:1713354/70‑1 (MQ=255)
                        tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                               <  1:3704535/71‑1 (MQ=255)
                         gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                                           >  1:3031072/1‑58 (MQ=255)
                         gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                                          >  1:1203666/1‑59 (MQ=255)
                          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                 >  1:1182642/1‑51 (MQ=255)
                          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                             <  1:482353/71‑1 (MQ=255)
                          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                             <  1:327095/71‑1 (MQ=255)
                          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccg                                              <  1:385379/70‑1 (MQ=255)
                            cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                                                <  1:2370356/66‑1 (MQ=255)
                              ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                       >  1:3059344/1‑57 (MQ=255)
                                aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                       <  1:755610/71‑1 (MQ=255)
                                aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                       >  1:1312345/1‑71 (MQ=255)
                                aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTACGCCGCAtt                                       <  1:3689283/71‑1 (MQ=255)
                                 tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                                   >  1:244553/1‑42 (MQ=255)
                                 tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                               <  1:188381/62‑1 (MQ=255)
                                  ttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                      <  1:2937035/70‑1 (MQ=255)
                                   tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                 >  1:1404608/1‑42 (MQ=255)
                                   tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                 >  1:2403525/1‑42 (MQ=255)
                                   tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                      <  1:2619489/69‑1 (MQ=255)
                                    gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                                      >  1:1220318/1‑52 (MQ=255)
                                     tACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCac                                   <  1:2748418/70‑2 (MQ=255)
                                     tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                   <  1:1846827/70‑1 (MQ=255)
                                     tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                   <  1:665244/70‑1 (MQ=255)
                                     tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                   <  1:660514/70‑1 (MQ=255)
                                       ccTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACAcc                                <  1:621551/71‑1 (MQ=255)
                                       ccTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACAcc                                <  1:3773520/71‑1 (MQ=255)
                                        cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCt                               <  1:3625457/71‑1 (MQ=255)
                                        cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCt                               <  1:3419973/71‑1 (MQ=255)
                                           tCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTg                              <  1:3655475/69‑1 (MQ=9)
                                             cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgcgc                           <  1:2887763/70‑1 (MQ=255)
                                               gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                                      <  1:212616/41‑1 (MQ=255)
                                               gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                                      <  1:1494542/41‑1 (MQ=255)
                                               gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCAATCGCGATTTCCGCCGCATTGCATACCTGCGCCg                         >  1:3141530/1‑70 (MQ=255)
                                                cgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCggg                       <  1:2687725/69‑2 (MQ=255)
                                                cgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCggg                       <  1:1670782/69‑2 (MQ=255)
                                                cAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                       <  1:1213022/39‑1 (MQ=255)
                                                 aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc                      >  1:513882/1‑71 (MQ=255)
                                                 aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc                      <  1:3014455/71‑1 (MQ=255)
                                                 aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc                      <  1:1732695/71‑1 (MQ=255)
                                                  aCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACt                     >  1:2419385/1‑71 (MQ=255)
                                                  aCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACt                     >  1:1686890/1‑71 (MQ=255)
                                                   ccGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCGGGCtt                    >  1:2433961/1‑70 (MQ=255)
                                                   ccGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAt                                                   <  1:610524/40‑1 (MQ=255)
                                                    cGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTgc                   >  1:613875/1‑71 (MQ=255)
                                                      gATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                                  >  1:556891/1‑38 (MQ=255)
                                                       aTCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                               <  1:3048837/40‑1 (MQ=255)
                                                          gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGccc              <  1:838594/70‑1 (MQ=255)
                                                           cAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCag            >  1:2013484/1‑71 (MQ=255)
                                                              gTCAGACCGAGGTTGTGCTCCATGGCGATTTccgcc                                            >  1:748225/1‑36 (MQ=19)
                                                              gTCAGACCGAGGTTGTGCTCCATGGCGATTTccgcc                                            >  1:2021829/1‑36 (MQ=19)
                                                              gTCAGACCGAGGTTGTGCTCCATGGCGATTTccgcc                                            >  1:179941/1‑36 (MQ=19)
                                                               tCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtagt        <  1:103575/71‑1 (MQ=255)
                                                                 aGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTTCATACCTGCGCCGGACTGCCGCCCAGTAGTTc      <  1:1713055/71‑1 (MQ=255)
                                                                 aGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt       >  1:328614/1‑70 (MQ=255)
                                                                  gCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCa     <  1:1277156/71‑1 (MQ=255)
                                                                   cccAAGGTTATGGTCCATCGCGATTTCCGCCGCATTGCATACCTgcgc                           >  1:2891604/1‑48 (MQ=255)
                                                                     cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCa     <  1:3106494/68‑1 (MQ=255)
                                                                     cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGtt  <  1:2743762/71‑1 (MQ=255)
                                                                     cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGt   >  1:1230071/1‑70 (MQ=255)
                                                                      aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagt           <  1:3016703/61‑1 (MQ=255)
                                                                      |                                                                     
CGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTT  >  minE/2093651‑2093790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑