Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,093,727 A→G 20.0% H375H (CAT→CAC)  tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,093,7270AG20.0% 157.9 / 33.8 75H375H (CAT→CAC) tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base A (20/40);  new base G (8/7);  total (28/47)
Fisher's exact test for biased strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

ATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCT  >  minE/2093659‑2093796
                                                                    |                                                                     
aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                       <  1:1192641/69‑1 (MQ=255)
aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                                      <  1:2990572/70‑1 (MQ=255)
  ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                                                                   >  1:2251416/1‑71 (MQ=255)
     aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                                                <  1:2510351/71‑1 (MQ=255)
     aTTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                                                <  1:2041893/71‑1 (MQ=255)
       tACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtg                                                              >  1:32290/1‑70 (MQ=255)
        aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                       >  1:3738059/1‑61 (MQ=255)
        aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                                                                  >  1:2898859/1‑66 (MQ=255)
        aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                                             >  1:432397/1‑69 (MQ=255)
        aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                                             >  1:3945273/1‑69 (MQ=255)
          gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgcg                                                           <  1:1594202/71‑1 (MQ=255)
          gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                             <  1:1090986/69‑1 (MQ=255)
          gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                             <  1:2607773/69‑1 (MQ=255)
              aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                                        <  1:1713354/70‑1 (MQ=255)
              aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                                        <  1:1763668/70‑1 (MQ=255)
                tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                                     <  1:3704535/71‑1 (MQ=255)
                 gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                                                 >  1:3031072/1‑58 (MQ=255)
                 gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCa                                                                >  1:1203666/1‑59 (MQ=255)
                  cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                       >  1:1182642/1‑51 (MQ=255)
                  cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                                   <  1:327095/71‑1 (MQ=255)
                  cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                                   <  1:482353/71‑1 (MQ=255)
                  cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccg                                                    <  1:385379/70‑1 (MQ=255)
                    cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                                                      <  1:2370356/66‑1 (MQ=255)
                      ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                             >  1:3059344/1‑57 (MQ=255)
                        aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                             >  1:1312345/1‑71 (MQ=255)
                        aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                             <  1:755610/71‑1 (MQ=255)
                        aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTACGCCGCAtt                                             <  1:3689283/71‑1 (MQ=255)
                         tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                                     <  1:188381/62‑1 (MQ=255)
                          ttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                            <  1:2937035/70‑1 (MQ=255)
                           tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                       >  1:1404608/1‑42 (MQ=255)
                           tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                       >  1:2403525/1‑42 (MQ=255)
                           tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                            <  1:2619489/69‑1 (MQ=255)
                            gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                                            >  1:1220318/1‑52 (MQ=255)
                             tACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCac                                         <  1:2748418/70‑2 (MQ=255)
                             tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                         <  1:665244/70‑1 (MQ=255)
                             tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                         <  1:660514/70‑1 (MQ=255)
                             tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                         <  1:1846827/70‑1 (MQ=255)
                               ccTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACAcc                                      <  1:621551/71‑1 (MQ=255)
                               ccTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACAcc                                      <  1:3773520/71‑1 (MQ=255)
                                cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCt                                     <  1:3419973/71‑1 (MQ=255)
                                cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCt                                     <  1:3625457/71‑1 (MQ=255)
                                   tCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTg                                    <  1:3655475/69‑1 (MQ=9)
                                     cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgcgc                                 <  1:2887763/70‑1 (MQ=255)
                                       gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                                            <  1:212616/41‑1 (MQ=255)
                                       gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                                            <  1:1494542/41‑1 (MQ=255)
                                       gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCAATCGCGATTTCCGCCGCATTGCATACCTGCGCCg                               >  1:3141530/1‑70 (MQ=255)
                                        cgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCggg                             <  1:2687725/69‑2 (MQ=255)
                                        cgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCggg                             <  1:1670782/69‑2 (MQ=255)
                                        cAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                             <  1:1213022/39‑1 (MQ=255)
                                         aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc                            <  1:3014455/71‑1 (MQ=255)
                                         aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc                            >  1:513882/1‑71 (MQ=255)
                                         aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc                            <  1:1732695/71‑1 (MQ=255)
                                          aCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACt                           >  1:1686890/1‑71 (MQ=255)
                                          aCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACt                           >  1:2419385/1‑71 (MQ=255)
                                           ccGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCGGGCtt                          >  1:2433961/1‑70 (MQ=255)
                                           ccGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAt                                                         <  1:610524/40‑1 (MQ=255)
                                            cGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTgc                         >  1:613875/1‑71 (MQ=255)
                                              gATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                                        >  1:556891/1‑38 (MQ=255)
                                               aTCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                                     <  1:3048837/40‑1 (MQ=255)
                                                  gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGccc                    <  1:838594/70‑1 (MQ=255)
                                                   cAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCag                  >  1:2013484/1‑71 (MQ=255)
                                                      gTCAGACCGAGGTTGTGCTCCATGGCGATTTccgcc                                                  >  1:179941/1‑36 (MQ=19)
                                                      gTCAGACCGAGGTTGTGCTCCATGGCGATTTccgcc                                                  >  1:748225/1‑36 (MQ=19)
                                                      gTCAGACCGAGGTTGTGCTCCATGGCGATTTccgcc                                                  >  1:2021829/1‑36 (MQ=19)
                                                       tCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtagt              <  1:103575/71‑1 (MQ=255)
                                                         aGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTTCATACCTGCGCCGGACTGCCGCCCAGTAGTTc            <  1:1713055/71‑1 (MQ=255)
                                                         aGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt             >  1:328614/1‑70 (MQ=255)
                                                          gCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCa           <  1:1277156/71‑1 (MQ=255)
                                                           cccAAGGTTATGGTCCATCGCGATTTCCGCCGCATTGCATACCTgcgc                                 >  1:2891604/1‑48 (MQ=255)
                                                             cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCa           <  1:3106494/68‑1 (MQ=255)
                                                             cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGtt        <  1:2743762/71‑1 (MQ=255)
                                                             cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGt         >  1:1230071/1‑70 (MQ=255)
                                                              aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagt                 <  1:3016703/61‑1 (MQ=255)
                                                               aGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgcgc                                 >  1:833009/1‑44 (MQ=255)
                                                               aGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTaa      >  1:143060/1‑71 (MQ=255)
                                                               aGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa       >  1:76089/1‑70 (MQ=255)
                                                                ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAc     <  1:265030/71‑1 (MQ=255)
                                                                 gTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCGGGCTTGCGCCTAATAGtt             <  1:3850049/62‑1 (MQ=255)
                                                                 gTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTg                          >  1:1920682/1‑49 (MQ=255)
                                                                  ttATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAccc   <  1:306517/71‑1 (MQ=255)
                                                                    aTGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCt  <  1:2570331/70‑1 (MQ=255)
                                                                    |                                                                     
ATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCT  >  minE/2093659‑2093796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑