Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,093,757 T→C 16.9% V365V (GTA→GTG)  tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,093,7570TC16.9% 148.4 / 22.3 65V365V (GTA→GTG) tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base T (34/20);  new base C (2/9);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 8.56e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

TACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAA  >  minE/2093688‑2093824
                                                                     |                                                                   
tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                                                     <  1:660514/70‑1 (MQ=255)
tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                                                     <  1:1846827/70‑1 (MQ=255)
tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                                                     <  1:665244/70‑1 (MQ=255)
  ccTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACAcc                                                                  <  1:3773520/71‑1 (MQ=255)
  ccTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACAcc                                                                  <  1:621551/71‑1 (MQ=255)
   cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCt                                                                 <  1:3419973/71‑1 (MQ=255)
   cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCt                                                                 <  1:3625457/71‑1 (MQ=255)
      tCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTg                                                                <  1:3655475/69‑1 (MQ=9)
        cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgcgc                                                             <  1:2887763/70‑1 (MQ=255)
          gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCAATCGCGATTTCCGCCGCATTGCATACCTGCGCCg                                                           >  1:3141530/1‑70 (MQ=255)
           cgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCggg                                                         <  1:2687725/69‑2 (MQ=255)
           cgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCggg                                                         <  1:1670782/69‑2 (MQ=255)
            aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc                                                        >  1:513882/1‑71 (MQ=255)
            aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc                                                        <  1:3014455/71‑1 (MQ=255)
            aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc                                                        <  1:1732695/71‑1 (MQ=255)
             aCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACt                                                       >  1:1686890/1‑71 (MQ=255)
             aCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACt                                                       >  1:2419385/1‑71 (MQ=255)
              ccGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCGGGCtt                                                      >  1:2433961/1‑70 (MQ=255)
               cGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTgc                                                     >  1:613875/1‑71 (MQ=255)
                     gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGccc                                                <  1:838594/70‑1 (MQ=255)
                      cAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCag                                              >  1:2013484/1‑71 (MQ=255)
                          tCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtagt                                          <  1:103575/71‑1 (MQ=255)
                            aGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTTCATACCTGCGCCGGACTGCCGCCCAGTAGTTc                                        <  1:1713055/71‑1 (MQ=255)
                            aGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt                                         >  1:328614/1‑70 (MQ=255)
                             gCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCa                                       <  1:1277156/71‑1 (MQ=255)
                              cccAAGGTTATGGTCCATCGCGATTTCCGCCGCATTGCATACCTgcgc                                                             >  1:2891604/1‑48 (MQ=255)
                                cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCa                                       <  1:3106494/68‑1 (MQ=255)
                                cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGtt                                    <  1:2743762/71‑1 (MQ=255)
                                cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGt                                     >  1:1230071/1‑70 (MQ=255)
                                 aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagt                                             <  1:3016703/61‑1 (MQ=255)
                                  aGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgcgc                                                             >  1:833009/1‑44 (MQ=255)
                                  aGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTaa                                  >  1:143060/1‑71 (MQ=255)
                                  aGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa                                   >  1:76089/1‑70 (MQ=255)
                                   ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAc                                 <  1:265030/71‑1 (MQ=255)
                                    gTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCGGGCTTGCGCCTAATAGtt                                         <  1:3850049/62‑1 (MQ=255)
                                    gTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTg                                                      >  1:1920682/1‑49 (MQ=255)
                                     ttATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAccc                               <  1:306517/71‑1 (MQ=255)
                                       aTGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCt                              <  1:2570331/70‑1 (MQ=255)
                                        tGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTg                             <  1:995383/70‑1 (MQ=255)
                                         gCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc                                                            <  1:2275269/38‑1 (MQ=255)
                                         gCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                                      >  1:2008961/1‑60 (MQ=255)
                                           tCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgc                         >  1:2338377/1‑71 (MQ=255)
                                            ccATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgcc                        >  1:902015/1‑71 (MQ=255)
                                            ccATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgcc                        >  1:3727431/1‑71 (MQ=255)
                                             cATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccg                       >  1:115773/1‑71 (MQ=255)
                                              aTCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgcc                           >  1:1887974/1‑66 (MQ=255)
                                               tCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgcc                     <  1:2749942/71‑1 (MQ=255)
                                                 gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCa                    <  1:3865026/70‑1 (MQ=255)
                                                 gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCAt                   >  1:2618907/1‑71 (MQ=255)
                                                 gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCAt                   >  1:1352718/1‑71 (MQ=255)
                                                 gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCAt                   >  1:790937/1‑71 (MQ=255)
                                                  cgATTTCCGCCGCGATGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCAtt                  <  1:31148/71‑1 (MQ=255)
                                                   gATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt                                         >  1:869469/1‑47 (MQ=255)
                                                    aTTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTg                 >  1:1832840/1‑70 (MQ=255)
                                                     tttCCGCCGCGATGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCAt                   <  1:546346/67‑1 (MQ=255)
                                                      ttCCGCCGCGATGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGag               >  1:3245122/1‑69 (MQ=255)
                                                      ttCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGtt                                    <  1:3523834/49‑1 (MQ=255)
                                                      ttCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTaa                                  >  1:3488973/1‑51 (MQ=255)
                                                       tCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTaa                                  >  1:319873/1‑50 (MQ=255)
                                                       tCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGaa               >  1:2260235/1‑69 (MQ=255)
                                                       tCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGaa               >  1:3228860/1‑69 (MQ=255)
                                                           ccgcATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt                                         >  1:2083737/1‑39 (MQ=255)
                                                           ccgcATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt                                         >  1:2461100/1‑39 (MQ=255)
                                                            cgcATTGCATACCTGCGCCGGACTTCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCa        >  1:1650097/1‑71 (MQ=255)
                                                                ttGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGc     >  1:3167169/1‑70 (MQ=255)
                                                                atGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGAGCacgccacgcc    <  1:788451/70‑1 (MQ=255)
                                                                  gCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTg                             >  1:1979594/1‑44 (MQ=255)
                                                                  gCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCaa  >  1:3864670/1‑71 (MQ=255)
                                                                     |                                                                   
TACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAA  >  minE/2093688‑2093824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑