Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,093,964 T→A 10.1% V296V (GTA→GTT)  tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,093,9640TA10.1% 245.5 / 17.2 89V296V (GTA→GTT) tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base T (46/34);  new base A (4/5);  total (50/39)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTT  >  minE/2093896‑2094034
                                                                    |                                                                      
gcgGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTAc                                                                      >  1:3672820/1‑71 (MQ=255)
gcgGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTAc                                                                      >  1:3834756/1‑71 (MQ=255)
gcgGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTAc                                                                      >  1:707839/1‑71 (MQ=255)
  ggCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCGGTACTg                                                                    >  1:2067798/1‑71 (MQ=255)
  ggCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTg                                                                    <  1:453454/71‑1 (MQ=255)
      aGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCgg                                                                <  1:2008851/71‑1 (MQ=255)
       gAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGAGGGAACTTATCGTAATAAGCCAGTACTGCCgg                                                                >  1:3508454/1‑70 (MQ=255)
       gAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCgg                                                                >  1:3325838/1‑70 (MQ=255)
       gAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCgg                                                                >  1:3519375/1‑70 (MQ=255)
       gAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCgg                                                                >  1:3729713/1‑70 (MQ=255)
       gAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCgg                                                                >  1:71886/1‑70 (MQ=255)
       gAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCgg                                                                >  1:488278/1‑70 (MQ=255)
         aaaTAGCGGTCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGaat                                                             <  1:3163321/71‑1 (MQ=255)
         aaaTAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGcc                                                                  <  1:3345717/66‑1 (MQ=255)
          aaTAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGaata                                                            <  1:1098770/71‑1 (MQ=255)
          aaTAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGaat                                                             >  1:630454/1‑70 (MQ=255)
              gCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCgg                                                                >  1:1668020/1‑63 (MQ=255)
                  gCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCcgca                                                    >  1:1548272/1‑71 (MQ=255)
                   cAATTGACCGCTCGTTTACCGGACGACGGAACTTAACGTAATAAGACAGTACTGCCga                                                                >  1:1531154/1‑57 (MQ=255)
                    aaTTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCg                                                                 <  1:768398/56‑1 (MQ=255)
                    aaTTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCcgcacg                                                  >  1:3103246/1‑71 (MQ=255)
                    aaTTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCcgcacg                                                  >  1:67203/1‑71 (MQ=255)
                     aTTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTa                                                                       <  1:2781963/49‑1 (MQ=255)
                       tGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCgcacgcac                                               >  1:2653440/1‑71 (MQ=255)
                        gACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCAcc                                              >  1:1605929/1‑71 (MQ=255)
                         aCCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCg                                             <  1:2274538/71‑1 (MQ=255)
                          ccGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGt                                            <  1:1093574/71‑1 (MQ=255)
                           cGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGt                                            >  1:2007860/1‑70 (MQ=255)
                           cGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGt                                            >  1:2012393/1‑70 (MQ=255)
                              tCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGcc                                                                  <  1:3528170/45‑1 (MQ=255)
                              tCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGt                                        >  1:3372359/1‑71 (MQ=255)
                              tCGTTTACCAGACGACGGAACTTATCGTAATAAGCCAGTACTGcc                                                                  <  1:1629083/45‑1 (MQ=255)
                                gTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTc                                       <  1:310760/70‑1 (MQ=255)
                                gTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGC‑‑CACGCACCGTTAGTCg                                      <  1:1137838/69‑1 (MQ=255)
                                gTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTAATGCCGGAATAATGCCGCACGCACCGTTAGTCg                                      >  1:3272342/1‑71 (MQ=255)
                                 tttACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCg                                             <  1:2611566/63‑1 (MQ=255)
                                   tACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGt                                    <  1:746612/70‑1 (MQ=255)
                                     ccGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGcc                                 <  1:1854728/71‑1 (MQ=255)
                                     ccGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGcc                                 <  1:3129887/71‑1 (MQ=255)
                                      cGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCg                                <  1:1144772/71‑1 (MQ=255)
                                      cGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCg                                >  1:2878945/1‑71 (MQ=255)
                                      cGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCg                                >  1:893794/1‑71 (MQ=255)
                                      cGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCg                                >  1:3357418/1‑71 (MQ=255)
                                       ggACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGt                               <  1:989600/71‑1 (MQ=255)
                                        gacgacGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGt                               >  1:3551870/1‑70 (MQ=255)
                                        gacgacGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGt                               >  1:409085/1‑70 (MQ=255)
                                          cgacgGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTAc                            <  1:1173665/71‑1 (MQ=255)
                                          cgacgGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTAc                            <  1:3261656/71‑1 (MQ=255)
                                           gacgGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTa                             <  1:3471859/69‑1 (MQ=255)
                                           gacgGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTAcc                           <  1:1039982/71‑1 (MQ=255)
                                           gacgGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTAcc                           <  1:84647/71‑1 (MQ=255)
                                           gacgGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTAcc                           <  1:3532993/71‑1 (MQ=255)
                                              taaaCTTGTCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTcaccac                         <  1:711265/68‑1 (MQ=255)
                                               gAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCAcgc                       <  1:186443/71‑1 (MQ=255)
                                                aaCTTGTCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCAcgc                       <  1:163734/70‑1 (MQ=255)
                                                aaCTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCAcgcg                      >  1:99484/1‑71 (MQ=255)
                                                aaCTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCAcgcg                      >  1:279286/1‑71 (MQ=255)
                                                 aCTTATCGTAATAAGCCAGTACTGCCGGAATAATGCcgcacgc                                                 <  1:3901322/43‑1 (MQ=255)
                                                  cTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGt                                            >  1:455183/1‑47 (MQ=255)
                                                  cTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTa                                          <  1:653536/49‑1 (MQ=255)
                                                  cTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACgcgc                     >  1:389199/1‑70 (MQ=255)
                                                  cTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcc                    >  1:2520396/1‑71 (MQ=255)
                                                  cTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcc                    >  1:2515975/1‑71 (MQ=255)
                                                   ttATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCAcg                        <  1:3082986/66‑1 (MQ=255)
                                                    tgtCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGcgaccg                  >  1:3615862/3‑71 (MQ=255)
                                                    tgtCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGcgacc                   >  1:3278248/3‑70 (MQ=255)
                                                     aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTc                                       >  1:3603563/1‑49 (MQ=255)
                                                     aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                 >  1:1539540/1‑71 (MQ=255)
                                                     aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                 >  1:1591342/1‑71 (MQ=255)
                                                     aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                 >  1:1624712/1‑71 (MQ=255)
                                                     aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                 >  1:73143/1‑71 (MQ=255)
                                                      tCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgcc                <  1:2693478/71‑1 (MQ=255)
                                                      tCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                 <  1:2267458/70‑1 (MQ=255)
                                                       cGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                 >  1:3070272/1‑69 (MQ=255)
                                                       cGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                 >  1:1069282/1‑69 (MQ=255)
                                                        gTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCgac                                           <  1:151593/42‑3 (MQ=255)
                                                        gTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTa                             >  1:1458611/1‑56 (MQ=255)
                                                        gTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAg              >  1:2664009/1‑71 (MQ=255)
                                                        gTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAg              <  1:885111/71‑1 (MQ=255)
                                                         taataaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGc             >  1:2931244/1‑71 (MQ=255)
                                                          aataaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgccc                   >  1:103838/1‑64 (MQ=255)
                                                              cgCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGt        <  1:878775/70‑1 (MQ=255)
                                                               gCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGtt       <  1:3639644/71‑1 (MQ=255)
                                                               gCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCTCAGTCACCACGCGACCGCCAGCAGCGtt       <  1:1300937/71‑1 (MQ=255)
                                                                ccAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGttc      <  1:344529/71‑2 (MQ=255)
                                                                 cAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCAcgcgac                    >  1:1228213/1‑54 (MQ=255)
                                                                 cAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGttct     >  1:3765492/1‑69 (MQ=255)
                                                                   gTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttct   >  1:3707312/1‑71 (MQ=255)
                                                                   gTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttct   >  1:2667918/1‑71 (MQ=255)
                                                                    tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt  <  1:3127909/71‑1 (MQ=255)
                                                                    |                                                                      
GCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTT  >  minE/2093896‑2094034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑