Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,093,973 A→G 19.0% I293I (ATT→ATC)  tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,093,9730AG19.0% 167.1 / 32.7 79I293I (ATT→ATC) tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base A (36/28);  new base G (8/7);  total (44/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAAC  >  minE/2093905‑2094041
                                                                    |                                                                    
aaaTAGCGGTCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGaat                                                                    <  1:3163321/71‑1 (MQ=255)
 aaTAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGaata                                                                   <  1:1098770/71‑1 (MQ=255)
 aaTAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGaat                                                                    >  1:630454/1‑70 (MQ=255)
         gCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCcgca                                                           >  1:1548272/1‑71 (MQ=255)
           aaTTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCcgcacg                                                         >  1:3103246/1‑71 (MQ=255)
           aaTTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCcgcacg                                                         >  1:67203/1‑71 (MQ=255)
              tGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCgcacgcac                                                      >  1:2653440/1‑71 (MQ=255)
               gACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCAcc                                                     >  1:1605929/1‑71 (MQ=255)
                aCCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCg                                                    <  1:2274538/71‑1 (MQ=255)
                 ccGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGt                                                   <  1:1093574/71‑1 (MQ=255)
                  cGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGt                                                   >  1:2007860/1‑70 (MQ=255)
                  cGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGt                                                   >  1:2012393/1‑70 (MQ=255)
                     tCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGt                                               >  1:3372359/1‑71 (MQ=255)
                       gTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTc                                              <  1:310760/70‑1 (MQ=255)
                       gTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGC‑‑CACGCACCGTTAGTCg                                             <  1:1137838/69‑1 (MQ=255)
                       gTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTAATGCCGGAATAATGCCGCACGCACCGTTAGTCg                                             >  1:3272342/1‑71 (MQ=255)
                        tttACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCg                                                    <  1:2611566/63‑1 (MQ=255)
                          tACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGt                                           <  1:746612/70‑1 (MQ=255)
                            ccGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGcc                                        <  1:3129887/71‑1 (MQ=255)
                            ccGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGcc                                        <  1:1854728/71‑1 (MQ=255)
                             cGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCg                                       >  1:3357418/1‑71 (MQ=255)
                             cGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCg                                       >  1:2878945/1‑71 (MQ=255)
                             cGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCg                                       >  1:893794/1‑71 (MQ=255)
                             cGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCg                                       <  1:1144772/71‑1 (MQ=255)
                              ggACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGt                                      <  1:989600/71‑1 (MQ=255)
                               gacgacGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGt                                      >  1:3551870/1‑70 (MQ=255)
                               gacgacGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGt                                      >  1:409085/1‑70 (MQ=255)
                                 cgacgGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTAc                                   <  1:1173665/71‑1 (MQ=255)
                                 cgacgGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTAc                                   <  1:3261656/71‑1 (MQ=255)
                                  gacgGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTa                                    <  1:3471859/69‑1 (MQ=255)
                                  gacgGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTAcc                                  <  1:3532993/71‑1 (MQ=255)
                                  gacgGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTAcc                                  <  1:84647/71‑1 (MQ=255)
                                  gacgGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTAcc                                  <  1:1039982/71‑1 (MQ=255)
                                     taaaCTTGTCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTcaccac                                <  1:711265/68‑1 (MQ=255)
                                      gAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCAcgc                              <  1:186443/71‑1 (MQ=255)
                                       aaCTTGTCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCAcgc                              <  1:163734/70‑1 (MQ=255)
                                       aaCTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCAcgcg                             >  1:99484/1‑71 (MQ=255)
                                       aaCTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCAcgcg                             >  1:279286/1‑71 (MQ=255)
                                        aCTTATCGTAATAAGCCAGTACTGCCGGAATAATGCcgcacgc                                                        <  1:3901322/43‑1 (MQ=255)
                                         cTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGt                                                   >  1:455183/1‑47 (MQ=255)
                                         cTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTa                                                 <  1:653536/49‑1 (MQ=255)
                                         cTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACgcgc                            >  1:389199/1‑70 (MQ=255)
                                         cTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcc                           >  1:2515975/1‑71 (MQ=255)
                                         cTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcc                           >  1:2520396/1‑71 (MQ=255)
                                          ttATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCAcg                               <  1:3082986/66‑1 (MQ=255)
                                           tgtCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGcgaccg                         >  1:3615862/3‑71 (MQ=255)
                                           tgtCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGcgacc                          >  1:3278248/3‑70 (MQ=255)
                                            aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTc                                              >  1:3603563/1‑49 (MQ=255)
                                            aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                        >  1:73143/1‑71 (MQ=255)
                                            aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                        >  1:1591342/1‑71 (MQ=255)
                                            aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                        >  1:1624712/1‑71 (MQ=255)
                                            aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                        >  1:1539540/1‑71 (MQ=255)
                                             tCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgcc                       <  1:2693478/71‑1 (MQ=255)
                                             tCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                        <  1:2267458/70‑1 (MQ=255)
                                              cGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                        >  1:3070272/1‑69 (MQ=255)
                                              cGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                        >  1:1069282/1‑69 (MQ=255)
                                               gTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCgac                                                  <  1:151593/42‑3 (MQ=255)
                                               gTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTa                                    >  1:1458611/1‑56 (MQ=255)
                                               gTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAg                     >  1:2664009/1‑71 (MQ=255)
                                               gTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAg                     <  1:885111/71‑1 (MQ=255)
                                                taataaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGc                    >  1:2931244/1‑71 (MQ=255)
                                                 aataaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgccc                          >  1:103838/1‑64 (MQ=255)
                                                     cgCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGt               <  1:878775/70‑1 (MQ=255)
                                                      gCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGtt              <  1:3639644/71‑1 (MQ=255)
                                                      gCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCTCAGTCACCACGCGACCGCCAGCAGCGtt              <  1:1300937/71‑1 (MQ=255)
                                                       ccAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGttc             <  1:344529/71‑2 (MQ=255)
                                                        cAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCAcgcgac                           >  1:1228213/1‑54 (MQ=255)
                                                        cAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGttct            >  1:3765492/1‑69 (MQ=255)
                                                          gaaCTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAg                     >  1:636282/3‑60 (MQ=255)
                                                          gTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttct          >  1:3707312/1‑71 (MQ=255)
                                                          gTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttct          >  1:2667918/1‑71 (MQ=255)
                                                           tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt         <  1:3127909/71‑1 (MQ=255)
                                                           aaCTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTctctt         >  1:3484239/2‑71 (MQ=255)
                                                            aCTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGcgacc                          >  1:3847039/1‑53 (MQ=255)
                                                             cTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTctcttcg       <  1:1568880/71‑2 (MQ=255)
                                                             cTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttcttc        >  1:1453895/1‑70 (MQ=255)
                                                               gCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTtctc           <  1:346085/65‑1 (MQ=255)
                                                               gCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt         >  1:1656806/1‑67 (MQ=255)
                                                                ccGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGtt              >  1:986529/1‑61 (MQ=255)
                                                                 cGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTaa   <  1:421292/71‑1 (MQ=255)
                                                                 cGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTaa   <  1:2554477/71‑1 (MQ=255)
                                                                  ggAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAAc  >  1:3107302/1‑71 (MQ=255)
                                                                    |                                                                    
AAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAAC  >  minE/2093905‑2094041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑