Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 311,478 A→T 11.3% A465A (GCT→GCA acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE311,4780AT11.3% 189.8 / 15.1 71A465A (GCT→GCAacrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (36/27);  new base T (6/2);  total (42/29)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

CAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACC  >  minE/311409‑311543
                                                                     |                                                                 
cAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGatagatag                                                                  >  1:2339641/1‑71 (MQ=255)
   gATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCAc                                                               >  1:2687185/1‑71 (MQ=255)
    aTCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCAcc                                                              <  1:907957/71‑1 (MQ=255)
         cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAg                                                            >  1:1344455/1‑68 (MQ=255)
         cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAg                                                            >  1:2798622/1‑68 (MQ=255)
         cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAg                                                            >  1:2204283/1‑68 (MQ=255)
         cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg                                                         <  1:3868617/71‑1 (MQ=255)
         cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg                                                         <  1:3281954/71‑1 (MQ=255)
          gCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCAc                                                               >  1:1353623/1‑64 (MQ=255)
          gCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg                                                         >  1:247677/1‑70 (MQ=255)
          gCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg                                                         >  1:2438780/1‑70 (MQ=255)
           ccaccaGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGaa                                                       <  1:2670857/71‑1 (MQ=255)
             accaGAACAGAAAGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAAcc                                                     >  1:666546/1‑71 (MQ=12)
               caGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGc                                                   >  1:2718405/1‑71 (MQ=255)
                  tACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa                                                 >  1:117680/1‑70 (MQ=255)
                   aCCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa                                                 >  1:2159153/1‑69 (MQ=255)
                   aCCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa                                                 >  1:2570316/1‑69 (MQ=255)
                    ccGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag                                              <  1:1279674/71‑1 (MQ=255)
                     cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga                                             <  1:1799279/71‑1 (MQ=255)
                     cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga                                             <  1:454905/71‑1 (MQ=255)
                     cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga                                             <  1:1460748/71‑1 (MQ=255)
                      gACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAg                                                            >  1:546196/1‑55 (MQ=255)
                      gACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa                                            <  1:3497770/71‑1 (MQ=255)
                          gcgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGc                                         <  1:3260098/70‑1 (MQ=255)
                           cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCa                                                                >  1:2331992/1‑46 (MQ=255)
                           cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAAcc                                                     <  1:630117/57‑1 (MQ=255)
                           cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa                                            >  1:1819940/1‑66 (MQ=255)
                           cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa                                            >  1:2682644/1‑66 (MQ=255)
                           cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCa                                       <  1:2867743/71‑1 (MQ=255)
                            gcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCAt                                      <  1:3408709/71‑1 (MQ=255)
                            gcCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCAt                                      <  1:2113064/71‑1 (MQ=9)
                             ccATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTa                                                          >  1:2686190/1‑50 (MQ=255)
                              cATTGCTGAAACAATGGTAATAGAGTACTGACGATAGATAGCACCAGTAGAAcc                                                     <  1:2778342/54‑1 (MQ=255)
                              cATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCg                                    >  1:2119889/1‑71 (MQ=255)
                               aTTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg                                   <  1:1450810/71‑1 (MQ=255)
                               aTTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg                                   <  1:3003775/71‑1 (MQ=255)
                                ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg                                   >  1:3866116/1‑70 (MQ=255)
                                ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg                                   >  1:414223/1‑70 (MQ=255)
                                 tGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa                                 >  1:3122340/1‑71 (MQ=255)
                                 tGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCGTCggaa                                 >  1:3936622/1‑69 (MQ=9)
                                  gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag                                              <  1:1593080/57‑1 (MQ=255)
                                  gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag                                              <  1:556139/57‑1 (MQ=255)
                                  gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag                                              <  1:2815359/57‑1 (MQ=255)
                                      aaaCGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCAt                                      >  1:2092038/1‑61 (MQ=9)
                                      aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGaa                                                       >  1:3263100/1‑44 (MQ=255)
                                      aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg                               <  1:2689047/68‑1 (MQ=255)
                                      aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGaa                             >  1:574802/1‑70 (MQ=255)
                                      aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAAt                            <  1:3545284/71‑1 (MQ=255)
                                       aaCGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGc                                                   >  1:1625577/1‑47 (MQ=12)
                                        aCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga                                             >  1:2491536/1‑52 (MQ=255)
                                         cAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAcc                         >  1:2666186/1‑71 (MQ=255)
                                           aTGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa                                            >  1:3392439/1‑50 (MQ=255)
                                            tGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgcc                      >  1:48657/1‑71 (MQ=255)
                                               tAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa                    >  1:3192846/1‑70 (MQ=255)
                                               tAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa                    >  1:2841052/1‑70 (MQ=255)
                                               tAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa                    >  1:702752/1‑70 (MQ=255)
                                                 aTAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCgaa                                               >  1:422278/1‑41 (MQ=12)
                                                  tAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa                                 >  1:410310/1‑54 (MQ=255)
                                                     agaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATa                           >  1:348051/1‑57 (MQ=255)
                                                     agaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAcc             <  1:2784490/71‑1 (MQ=255)
                                                      gaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa            >  1:960071/1‑71 (MQ=255)
                                                       aaCTGGCGATAAATTGCCCCAGTAGAACCGCCgaagaa                                            >  1:2747381/1‑38 (MQ=12)
                                                       aaCTGACGATAGATAGCACCAGTAGAACCGCCAaagaa                                            >  1:3022234/1‑38 (MQ=255)
                                                        aCTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCAt           <  1:880954/70‑1 (MQ=9)
                                                        aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCAt           <  1:2531350/70‑1 (MQ=255)
                                                        aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATc          <  1:3754158/71‑1 (MQ=255)
                                                        aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATc          <  1:2104716/71‑1 (MQ=255)
                                                        aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCAGCGGTACGAATACCGCCGACAGTACCATc          <  1:1121581/71‑1 (MQ=255)
                                                         atGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcg         >  1:1725338/2‑71 (MQ=255)
                                                          tGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcg         >  1:2672090/1‑70 (MQ=255)
                                                          tGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGAACCAt           <  1:1535646/68‑1 (MQ=255)
                                                            aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa      >  1:1412848/1‑71 (MQ=255)
                                                              gatagataGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATa    >  1:2727115/1‑71 (MQ=255)
                                                              gatagataGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATa    <  1:1264754/71‑1 (MQ=255)
                                                                tagatagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgcc                      <  1:443508/51‑1 (MQ=255)
                                                                tagatagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATAcc  <  1:812398/71‑1 (MQ=255)
                                                                     |                                                                 
CAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACC  >  minE/311409‑311543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: