| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,478 | A→T | 11.3% | A465A (GCT→GCA) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,478 | 0 | A | T | 11.3% | 189.8 / 15.1 | 71 | A465A (GCT→GCA) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (36/27); new base T (6/2); total (42/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01 | |||||||||||
CAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACC > minE/311409‑311543 | cAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGatagatag > 1:2339641/1‑71 (MQ=255) gATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCAc > 1:2687185/1‑71 (MQ=255) aTCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCAcc < 1:907957/71‑1 (MQ=255) cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAg > 1:1344455/1‑68 (MQ=255) cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAg > 1:2798622/1‑68 (MQ=255) cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAg > 1:2204283/1‑68 (MQ=255) cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg < 1:3868617/71‑1 (MQ=255) cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg < 1:3281954/71‑1 (MQ=255) gCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCAc > 1:1353623/1‑64 (MQ=255) gCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg > 1:247677/1‑70 (MQ=255) gCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg > 1:2438780/1‑70 (MQ=255) ccaccaGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGaa < 1:2670857/71‑1 (MQ=255) accaGAACAGAAAGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAAcc > 1:666546/1‑71 (MQ=12) caGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGc > 1:2718405/1‑71 (MQ=255) tACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa > 1:117680/1‑70 (MQ=255) aCCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa > 1:2159153/1‑69 (MQ=255) aCCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa > 1:2570316/1‑69 (MQ=255) ccGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag < 1:1279674/71‑1 (MQ=255) cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga < 1:1799279/71‑1 (MQ=255) cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga < 1:454905/71‑1 (MQ=255) cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga < 1:1460748/71‑1 (MQ=255) gACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAg > 1:546196/1‑55 (MQ=255) gACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa < 1:3497770/71‑1 (MQ=255) gcgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGc < 1:3260098/70‑1 (MQ=255) cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCa > 1:2331992/1‑46 (MQ=255) cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAAcc < 1:630117/57‑1 (MQ=255) cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa > 1:1819940/1‑66 (MQ=255) cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa > 1:2682644/1‑66 (MQ=255) cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCa < 1:2867743/71‑1 (MQ=255) gcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCAt < 1:3408709/71‑1 (MQ=255) gcCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCAt < 1:2113064/71‑1 (MQ=9) ccATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTa > 1:2686190/1‑50 (MQ=255) cATTGCTGAAACAATGGTAATAGAGTACTGACGATAGATAGCACCAGTAGAAcc < 1:2778342/54‑1 (MQ=255) cATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCg > 1:2119889/1‑71 (MQ=255) aTTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg < 1:1450810/71‑1 (MQ=255) aTTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg < 1:3003775/71‑1 (MQ=255) ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg > 1:3866116/1‑70 (MQ=255) ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg > 1:414223/1‑70 (MQ=255) tGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa > 1:3122340/1‑71 (MQ=255) tGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCGTCggaa > 1:3936622/1‑69 (MQ=9) gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag < 1:1593080/57‑1 (MQ=255) gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag < 1:556139/57‑1 (MQ=255) gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag < 1:2815359/57‑1 (MQ=255) aaaCGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCAt > 1:2092038/1‑61 (MQ=9) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGaa > 1:3263100/1‑44 (MQ=255) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:2689047/68‑1 (MQ=255) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGaa > 1:574802/1‑70 (MQ=255) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAAt < 1:3545284/71‑1 (MQ=255) aaCGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGc > 1:1625577/1‑47 (MQ=12) aCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga > 1:2491536/1‑52 (MQ=255) cAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAcc > 1:2666186/1‑71 (MQ=255) aTGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa > 1:3392439/1‑50 (MQ=255) tGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgcc > 1:48657/1‑71 (MQ=255) tAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa > 1:3192846/1‑70 (MQ=255) tAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa > 1:2841052/1‑70 (MQ=255) tAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa > 1:702752/1‑70 (MQ=255) aTAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCgaa > 1:422278/1‑41 (MQ=12) tAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa > 1:410310/1‑54 (MQ=255) agaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATa > 1:348051/1‑57 (MQ=255) agaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAcc < 1:2784490/71‑1 (MQ=255) gaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa > 1:960071/1‑71 (MQ=255) aaCTGGCGATAAATTGCCCCAGTAGAACCGCCgaagaa > 1:2747381/1‑38 (MQ=12) aaCTGACGATAGATAGCACCAGTAGAACCGCCAaagaa > 1:3022234/1‑38 (MQ=255) aCTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCAt < 1:880954/70‑1 (MQ=9) aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCAt < 1:2531350/70‑1 (MQ=255) aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATc < 1:3754158/71‑1 (MQ=255) aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATc < 1:2104716/71‑1 (MQ=255) aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCAGCGGTACGAATACCGCCGACAGTACCATc < 1:1121581/71‑1 (MQ=255) atGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcg > 1:1725338/2‑71 (MQ=255) tGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcg > 1:2672090/1‑70 (MQ=255) tGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGAACCAt < 1:1535646/68‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa > 1:1412848/1‑71 (MQ=255) gatagataGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATa > 1:2727115/1‑71 (MQ=255) gatagataGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATa < 1:1264754/71‑1 (MQ=255) tagatagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgcc < 1:443508/51‑1 (MQ=255) tagatagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATAcc < 1:812398/71‑1 (MQ=255) | CAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACC > minE/311409‑311543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |