Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,094,024 T→A 16.7% A276A (GCA→GCT)  tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,094,0240TA16.7% 148.2 / 22.0 60A276A (GCA→GCT) tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base T (25/25);  new base A (3/7);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 3.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.83e-01

AGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAG  >  minE/2093958‑2094093
                                                                  |                                                                     
cgCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGt                                                                   <  1:878775/70‑1 (MQ=255)
 gCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGtt                                                                  <  1:3639644/71‑1 (MQ=255)
 gCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCTCAGTCACCACGCGACCGCCAGCAGCGtt                                                                  <  1:1300937/71‑1 (MQ=255)
  ccAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGttc                                                                 <  1:344529/71‑2 (MQ=255)
   cAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGttct                                                                >  1:3765492/1‑69 (MQ=255)
     gTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttct                                                              >  1:2667918/1‑71 (MQ=255)
     gTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttct                                                              >  1:3707312/1‑71 (MQ=255)
      tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                             <  1:3127909/71‑1 (MQ=255)
      aaCTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTctctt                                                             >  1:3484239/2‑71 (MQ=255)
        cTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTctcttcg                                                           <  1:1568880/71‑2 (MQ=255)
        cTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttcttc                                                            >  1:1453895/1‑70 (MQ=255)
          gCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTtctc                                                               <  1:346085/65‑1 (MQ=255)
          gCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                             >  1:1656806/1‑67 (MQ=255)
           ccGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGtt                                                                  >  1:986529/1‑61 (MQ=255)
            cGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTaa                                                       <  1:421292/71‑1 (MQ=255)
            cGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTaa                                                       <  1:2554477/71‑1 (MQ=255)
             ggAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAAc                                                      >  1:3107302/1‑71 (MQ=255)
                     gCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc                                                   <  1:1832254/66‑1 (MQ=255)
                        gcacgcacCGTTAGTCGGTGCGGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGcgcg                                            >  1:777986/1‑70 (MQ=255)
                        gcacgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAgcgc                                             >  1:1339390/1‑69 (MQ=255)
                         cacgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGt                                           >  1:2436712/1‑70 (MQ=255)
                            gcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc                                                   <  1:1597629/59‑1 (MQ=255)
                            gcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAgcg                                              <  1:1285285/64‑1 (MQ=255)
                            gcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACAt                                       >  1:1447402/1‑71 (MQ=255)
                             cacCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAGTGCAAACATg                                      <  1:1704461/71‑1 (MQ=255)
                                cGTTAGTCGGTGCCGTGACCACGCGCCCGCCAGCTGCGTTttct                                                              >  1:56041/1‑44 (MQ=255)
                                 gTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACt                                                         <  1:1731682/48‑1 (MQ=255)
                                 gTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCTCCAGCGCGTACATGTTGa                                  <  1:3195049/71‑1 (MQ=255)
                                   tAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATc                                >  1:3610100/1‑71 (MQ=255)
                                    aGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATc                                <  1:745603/70‑1 (MQ=255)
                                    aGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATc                                <  1:2531017/70‑1 (MQ=255)
                                     gTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa                              <  1:1597341/71‑1 (MQ=255)
                                      tCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGtt                                    <  1:844842/64‑1 (MQ=255)
                                            ccGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGAt                        >  1:291964/1‑70 (MQ=255)
                                            ccGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGAt                        <  1:1377253/70‑1 (MQ=255)
                                            ccGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGAt                        <  1:2473296/70‑1 (MQ=255)
                                             cGTTACGACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                 >  1:3359/1‑60 (MQ=255)
                                             cGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                 >  1:1839150/1‑60 (MQ=255)
                                              gTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGa                                  <  1:189948/58‑1 (MQ=255)
                                               ttACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACa                    >  1:3949123/1‑71 (MQ=255)
                                                tACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAt                   >  1:1978962/1‑71 (MQ=255)
                                                   cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACAt                                       <  1:773673/48‑1 (MQ=255)
                                                   cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCa                <  1:3628263/71‑1 (MQ=255)
                                                   cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCa                >  1:3487004/1‑71 (MQ=255)
                                                   cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCa                <  1:963412/71‑1 (MQ=255)
                                                    aCGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt                  >  1:1038050/1‑68 (MQ=255)
                                                     cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATc              >  1:2447557/1‑71 (MQ=255)
                                                     cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATc              >  1:2627380/1‑71 (MQ=255)
                                                     cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATc              >  1:1682194/1‑71 (MQ=255)
                                                     cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGAAGACATTCATc              >  1:535073/1‑71 (MQ=255)
                                                      gcgcCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGt                            >  1:3897946/1‑56 (MQ=255)
                                                        taccgccAGCAGCGTTCTCTTCTTTCACTGCCAGTGCAAACATGTTGATcc                               <  1:746924/49‑1 (MQ=255)
                                                        gcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCgg            <  1:2891269/70‑1 (MQ=255)
                                                            gccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATTTTGATCCAGTCGATGACATTCATCGGATCGt       <  1:2594171/71‑1 (MQ=255)
                                                            gccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATTGGAt          >  1:1651931/1‑68 (MQ=255)
                                                            gccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGt       <  1:3509752/71‑1 (MQ=255)
                                                               aGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGt       >  1:597786/1‑68 (MQ=255)
                                                               aGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTag    <  1:3191306/71‑1 (MQ=255)
                                                                 cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTagag  <  1:2676837/71‑1 (MQ=255)
                                                                 cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTagag  <  1:3771677/71‑1 (MQ=255)
                                                                  |                                                                     
AGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAG  >  minE/2093958‑2094093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑