| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,094,822 | A→G | 7.3% | I10I (ATT→ATC) | tdcG ← | L‑serine dehydratase 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,094,822 | 0 | A | G | 7.3% | 228.7 / 7.6 | 82 | I10I (ATT→ATC) | tdcG | L‑serine dehydratase 3 |
| Reads supporting (aligned to +/- strand): ref base A (49/27); new base G (2/4); total (51/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01 | |||||||||||
AGCCGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAA > minE/2094754‑2094892 | aGCCGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAAt < 1:2269486/71‑1 (MQ=255) ccGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAAttt < 1:2829431/71‑1 (MQ=255) cAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCa > 1:2769049/1‑62 (MQ=255) aaaCTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATc > 1:1161045/1‑71 (MQ=255) ttgtttaCCCGCTTTCATTGGTCCAACGGTATGAGAACTGGAAGGGCCAATGCCGATTTTGAAAATATCg > 1:716263/4‑70 (MQ=255) cTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGaa > 1:1677234/1‑71 (MQ=255) cTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGaa > 1:1424773/1‑71 (MQ=255) tttttCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCa < 1:666668/53‑1 (MQ=255) ttttCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCa > 1:1399229/1‑52 (MQ=255) ttttCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg > 1:2781429/1‑71 (MQ=255) ttttCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCACAATTTTGAAAATATCGAATg > 1:2286589/1‑71 (MQ=255) tttCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCaa > 1:3692426/1‑52 (MQ=255) tttCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg > 1:2389924/1‑70 (MQ=255) tttCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg > 1:691851/1‑70 (MQ=255) gctttcattGGTCCAACGGTATGAGAACTGGAAGGGCCAATGCCGATTTTGAAAATATCGAATACGCTAAt > 1:2976114/4‑71 (MQ=255) cattcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg > 1:2490450/1‑63 (MQ=255) tttcattGGTCCAACGGTATGAGAACTGGAAGGGCCAATGCCGATTTTGAAAATATCGaa < 1:3824543/59‑1 (MQ=255) attcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGa > 1:1959186/1‑50 (MQ=255) ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGaaaa > 1:324940/1‑52 (MQ=255) tcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCata > 1:3131267/1‑71 (MQ=255) cattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCatat > 1:919355/1‑71 (MQ=255) attGGTCCAACGGTATGAGAACTGGAAGGGCCAATGCCGATTTTGAAAATATCGAAtac < 1:2428345/59‑3 (MQ=255) attGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCg < 1:430453/54‑1 (MQ=255) ttGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAat < 1:1707496/49‑1 (MQ=255) tGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAata > 1:2180987/1‑49 (MQ=255) tGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCca > 1:1812294/1‑71 (MQ=255) ccccACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGaaaa < 1:1680818/44‑1 (MQ=255) cccACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGaaa < 1:514929/42‑1 (MQ=255) ccACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGaa < 1:2947819/40‑1 (MQ=255) cGGTATGCGAAGTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCcaca > 1:203314/1‑65 (MQ=255) cGGTATGCGAACTGGAGGGACCAATCCCAATTTTGaaaa > 1:2068757/1‑39 (MQ=255) cGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGaa > 1:1134163/1‑46 (MQ=255) cGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCtag > 1:807733/1‑69 (MQ=255) cGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCg < 1:2638756/71‑1 (MQ=255) cGGTATGCGAACTGGAGGGACCAATCCAAATTTTGAAAATATAGa > 1:2155893/1‑45 (MQ=38) gTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAat > 1:2169022/1‑38 (MQ=255) gTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAat > 1:1159900/1‑38 (MQ=255) gTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAat > 1:1799083/1‑38 (MQ=255) gTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTa < 1:3131447/51‑1 (MQ=255) gTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGa < 1:2363500/71‑1 (MQ=255) tATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACAccc < 1:1408336/65‑1 (MQ=255) tATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGAt < 1:908722/71‑1 (MQ=255) aTGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGAtt > 1:2188270/1‑71 (MQ=255) aTGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGAtt > 1:904506/1‑71 (MQ=255) gCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATc > 1:1757316/1‑55 (MQ=255) cGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGcc > 1:2267187/1‑71 (MQ=255) gAACTTGAGGGACCAATCCCAATTTTGAAAATATCg < 1:2000894/36‑1 (MQ=255) gAACTGGAGGGACCAATCCCAATTTTGAAAATATCGaa > 1:2172706/1‑38 (MQ=255) gAACTGGAAGGGCCAATGCCGATTTTGAAAATATCGAATACGCTAATCa < 1:939699/49‑1 (MQ=19) gAACTGGAAGGGCCAATGCCGATTTTGAAAATATCGAATACGCTAATCa < 1:1218923/49‑1 (MQ=19) aaCTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCata > 1:1754043/1‑50 (MQ=255) aCTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGtt > 1:2632604/1‑71 (MQ=255) aCTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGtt > 1:2097949/1‑71 (MQ=255) cTGGAGGGACCAATCCCAATTTTGAAAATATCGaa > 1:2582/1‑35 (MQ=255) cTGGAGGGACCAATCCCAATTTTGAAAATATCGaa > 1:3097608/1‑35 (MQ=255) cTGGAGGGACCAATCCCAATATTGAAAATATCGaa > 1:1620267/1‑35 (MQ=255) gAGGGACCAATCCCAATTTTGAAAATATCGAATGCAc < 1:3468271/37‑1 (MQ=255) gAGGGACCAATCCCAATTTTGAAAATATCGAATGCAc < 1:3471092/37‑1 (MQ=255) gAGGGACCAATCCCAATTTTGAAAATATCGAATGCAc < 1:2924990/37‑1 (MQ=255) gAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGa > 1:1126004/1‑59 (MQ=255) aGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTg < 1:1981665/71‑1 (MQ=255) gggACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTg > 1:284978/1‑70 (MQ=255) ggACCAATCCCAATTTTGAAAATATCGAATGCACTAATCata < 1:2819680/42‑1 (MQ=255) ggACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTg > 1:1828808/1‑59 (MQ=255) gACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAg < 1:3177156/71‑1 (MQ=255) aCCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAg < 1:3632857/70‑1 (MQ=255) aCCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAg < 1:2163052/70‑1 (MQ=255) aCCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAg < 1:3016498/70‑1 (MQ=255) aCCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGt < 1:3189754/71‑1 (MQ=255) cAATCCCAATTTTGAAAATATCGAATGCACTAATCATATc > 1:3277506/1‑40 (MQ=255) cAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTc > 1:2891123/1‑61 (MQ=255) cAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTg > 1:3613049/1‑70 (MQ=255) cAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTg > 1:1177845/1‑70 (MQ=255) tCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTc > 1:3364384/1‑46 (MQ=255) cccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGt > 1:735780/1‑55 (MQ=255) ccAATTTTGAAAATATCGAATGCACTAATCATATCcaca > 1:1604397/1‑39 (MQ=255) ccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGtt > 1:1896096/1‑55 (MQ=255) ccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCa > 1:2932591/1‑57 (MQ=255) ccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCgg < 1:233942/71‑1 (MQ=255) ccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCg < 1:3521026/70‑1 (MQ=255) ccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCg > 1:1944920/1‑70 (MQ=255) ccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCg > 1:1806156/1‑70 (MQ=255) acAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCa > 1:376260/2‑57 (MQ=255) cAATTTTGAAAATATCGAATGCACTAATCATATcc < 1:2460406/35‑1 (MQ=255) cAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCgg > 1:1523832/1‑70 (MQ=255) cAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGa < 1:2499155/71‑1 (MQ=255) aaTTTTGAAAATATCGAATGCACTAATCATTTCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAg > 1:1383961/1‑66 (MQ=255) aaTTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGaa > 1:653196/1‑71 (MQ=255) aaTTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGaa > 1:1920585/1‑71 (MQ=255) | AGCCGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAA > minE/2094754‑2094892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |