| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,111,795 | T→C | 11.3% | C147R (TGC→CGC) | agaI → | galactosamine‑6‑phosphate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,111,795 | 0 | T | C | 11.3% | 163.8 / 11.1 | 62 | C147R (TGC→CGC) | agaI | galactosamine‑6‑phosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base T (27/28); new base C (2/5); total (29/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
CGAAGAGATAAATGAGACAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGC > minE/2111725‑2111862 | cGAATAGATAAATGAGACAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTAt < 1:640025/71‑1 (MQ=255)cGAAGAGATAAATGAGACAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTAt < 1:3882548/71‑1 (MQ=255)cGAAGAGATAAATGAGACAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTAt < 1:2980975/71‑1 (MQ=255) aaGAGATAAATGAGACAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGc < 1:1041542/71‑1 (MQ=255) aaGAGATAAATGAGACAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGc > 1:644557/1‑71 (MQ=255) agagATAAATGAGACAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCg > 1:3100691/1‑71 (MQ=255) gaTAAATGAGACAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTtc < 1:708232/71‑1 (MQ=255) aTAAATGAGACAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTAt < 1:1466637/64‑1 (MQ=255) aTAAATGAGACAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTtct > 1:950270/1‑71 (MQ=255) aaaTGAGACAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCg > 1:3153107/1‑71 (MQ=255) aaaTGAGACAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCg > 1:778634/1‑71 (MQ=255) aTGAGACAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTtctc > 1:2913287/1‑68 (MQ=255) aTGAGACAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGa > 1:1673101/1‑71 (MQ=255) gagaCAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGAtt > 1:715248/1‑71 (MQ=255) agaCAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGAtt < 1:905300/70‑1 (MQ=255) agaCAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGAtt < 1:3347643/70‑1 (MQ=255) agaCAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGAtt < 1:1788043/70‑1 (MQ=255) agaCAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTg < 1:3219755/71‑1 (MQ=255) agaCAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTg > 1:3594332/1‑71 (MQ=255) agaCAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGAtt < 1:505161/70‑1 (MQ=255) agaCAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGG‑‑TGGATTTATGCGTTCTCGGATTggg > 1:2239206/1‑71 (MQ=255) aCAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTAt < 1:1375679/55‑1 (MQ=255) aCAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTggg > 1:3587243/1‑71 (MQ=255) gagTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGa > 1:1071999/1‑70 (MQ=255) agTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGAtt < 1:2814109/64‑1 (MQ=255) agTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGaa > 1:3009682/1‑70 (MQ=255) gTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGaaaa < 1:953016/71‑1 (MQ=255) gAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACgg < 1:2276229/71‑1 (MQ=255) gAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACg < 1:3205511/70‑1 (MQ=255) aaCGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGaaaaa < 1:1256384/67‑1 (MQ=255) aCGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACg > 1:1640393/1‑68 (MQ=255) aCGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGt > 1:2271960/1‑70 (MQ=255) aCGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGt > 1:846350/1‑70 (MQ=255) cGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTgg > 1:3538899/1‑58 (MQ=255) ggTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGa < 1:1093197/52‑1 (MQ=255) gTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGaaaa < 1:1222740/61‑1 (MQ=255) tAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCtt < 1:2441735/71‑1 (MQ=255) tAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCtt < 1:2080414/71‑1 (MQ=255) tAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGTCATCtt < 1:2251126/71‑1 (MQ=255) aaCGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTAt > 1:2274456/1‑38 (MQ=255) aaCGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTAt > 1:1094609/1‑38 (MQ=255) aCGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTgg > 1:510844/1‑71 (MQ=255) aCGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTg < 1:1807811/70‑1 (MQ=255) aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTtc < 1:1132688/40‑1 (MQ=255) aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCt > 1:1403750/1‑71 (MQ=255) ccTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCAt < 1:424567/61‑1 (MQ=255) ccTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTg > 1:1865114/1‑70 (MQ=255) cTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTgaa > 1:1467341/1‑71 (MQ=255) gATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTgaac > 1:3910703/1‑70 (MQ=255) aTTGCGCGCAAAGGCTGTCTGGATTTATGCGTTCTCGGAtt < 1:2939162/41‑1 (MQ=255) gcgcgcAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTgaacgaac < 1:2349877/70‑1 (MQ=255) gcgcAAAGGCGGTCTGGATTTATGCGTTCTCGTATTg < 1:1820068/37‑1 (MQ=255) gcgcAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACgg > 1:2970056/1‑48 (MQ=255) cAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCgg > 1:3106429/1‑68 (MQ=255) cAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGgag < 1:293985/71‑1 (MQ=255) cAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGgag < 1:1440076/71‑1 (MQ=255) aaaGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGgaga < 1:1319711/71‑1 (MQ=255) aaGGCGGTCTGGATTTATGCGTTCTCGGATTgggg < 1:953597/35‑1 (MQ=255) aGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCt < 1:1232593/48‑1 (MQ=255) ggCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATc < 1:3255008/46‑1 (MQ=255) ggCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAg < 1:3602637/71‑1 (MQ=255) gCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGc < 1:912596/71‑1 (MQ=255) tCTGGATTTATGCGTTCTCGGATTGGGGAAAAACg < 1:2235858/35‑1 (MQ=255) cTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGc > 1:3904547/1‑70 (MQ=255) tGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCaa > 1:1593727/1‑71 (MQ=255) aTTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAAcc < 1:3177171/52‑1 (MQ=255) tttATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCgg > 1:3207800/1‑71 (MQ=255) ttATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGCACCGGGAGAAAGCCTGCAACCGGc > 1:3918506/1‑71 (MQ=255) tATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTgaacgaa > 1:733243/1‑47 (MQ=255) | CGAAGAGATAAATGAGACAGAGTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGC > minE/2111725‑2111862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |