Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,130,584 A→G 9.4% I107I (ATT→ATC mtr ← tryptophan transporter of high affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,130,5840AG 9.4% 205.7 / 9.5 74I107I (ATT→ATCmtrtryptophan transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base A (29/38);  new base G (5/2);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGCCCGTGCCGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTG  >  minE/2130516‑2130653
                                                                    |                                                                     
gccgccCGTGCCGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGaa                                                                       >  1:2260449/1‑69 (MQ=255)
gccgccCGTGCCGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAat                                                                     <  1:1132620/71‑1 (MQ=255)
  cgccCGTGCCGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAatat                                                                   <  1:708268/71‑1 (MQ=255)
     ccGTGCCGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAgg                                                                >  1:589502/1‑71 (MQ=255)
        tGCCGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCAt                                                             <  1:3247569/71‑1 (MQ=255)
           cGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAat                                                                     >  1:2363793/1‑60 (MQ=255)
           cGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAat                                                                     >  1:3012531/1‑60 (MQ=255)
            gggACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAg                                                                 >  1:3473356/1‑63 (MQ=255)
               aCGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAg                                                           <  1:1396721/66‑1 (MQ=255)
                    tAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGata                                                  >  1:52062/1‑70 (MQ=255)
                      gTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGata                                                  >  1:3497767/1‑68 (MQ=255)
                        gACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATagag                                             >  1:2327900/1‑71 (MQ=255)
                         aCATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATagag                                             >  1:238406/1‑70 (MQ=255)
                          cATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                           <  1:829437/71‑1 (MQ=255)
                           aTCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGt                                                         <  1:2843739/56‑1 (MQ=255)
                           aTCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                           >  1:2079159/1‑70 (MQ=255)
                               ctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCa                                                       >  1:2219800/1‑54 (MQ=255)
                                tGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGaa                                                                       <  1:1150494/37‑1 (MQ=255)
                                tGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGaa                                                                       <  1:2885209/37‑1 (MQ=255)
                                tGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGcaca                                         <  1:2026017/67‑1 (MQ=255)
                                tGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGcaca                                         <  1:3570034/67‑1 (MQ=255)
                                tGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGcaca                                         <  1:1317160/67‑1 (MQ=255)
                                   gAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGGCAGGATATaga                                              <  1:2413942/59‑1 (MQ=255)
                                      ggTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATg                                >  1:2699596/1‑70 (MQ=255)
                                       gtgtGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATgg                               >  1:1830348/1‑70 (MQ=255)
                                         gtgATTCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCa                                                       <  1:974314/44‑1 (MQ=255)
                                         gtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACaa                                        <  1:1000448/59‑1 (MQ=255)
                                          tgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCaa                                  >  1:1259575/1‑64 (MQ=255)
                                          tgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAAt                           >  1:816929/1‑71 (MQ=255)
                                          tgAAGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCa                                   >  1:1606479/1‑63 (MQ=255)
                                           gATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGt                                                         <  1:1155305/40‑1 (MQ=255)
                                           gATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAg                                                      <  1:901283/43‑1 (MQ=255)
                                            aTGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGc                         >  1:3756300/1‑71 (MQ=255)
                                               cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGa                                                    >  1:1911112/1‑41 (MQ=255)
                                               cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACaa                                        >  1:1656897/1‑53 (MQ=255)
                                               cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCg                       <  1:313305/70‑1 (MQ=255)
                                               cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCg                       <  1:3419028/70‑1 (MQ=255)
                                               cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCg                       <  1:2476653/70‑1 (MQ=255)
                                                aGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                     >  1:3841630/1‑71 (MQ=255)
                                                 gAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                     >  1:3882045/1‑70 (MQ=255)
                                                 gAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTg                    <  1:3419420/71‑1 (MQ=255)
                                                  aaTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCa                                   <  1:2064659/55‑1 (MQ=255)
                                                  aaTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCa                                   <  1:1998809/55‑1 (MQ=255)
                                                   aTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGa                   >  1:2638131/1‑70 (MQ=255)
                                                   aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGt                      <  1:2570390/67‑1 (MQ=255)
                                                   aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGt                      <  1:220924/67‑1 (MQ=255)
                                                    tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAAt                                 >  1:615111/1‑55 (MQ=255)
                                                    tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc                  >  1:1333389/1‑70 (MQ=255)
                                                    tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc                  >  1:2752857/1‑70 (MQ=255)
                                                     cGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGaa                             <  1:2238363/58‑1 (MQ=255)
                                                      gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCAc               >  1:431476/1‑71 (MQ=255)
                                                      gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCAc               >  1:3038275/1‑71 (MQ=255)
                                                       caCCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAAta                          >  1:578192/2‑58 (MQ=255)
                                                       caCCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAAta                          >  1:2801671/2‑58 (MQ=255)
                                                       aacaacTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATgg                               <  1:2188696/54‑1 (MQ=255)
                                                        aCCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACgaa                                       >  1:3037448/1‑45 (MQ=255)
                                                        aCCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAAtac                         >  1:2706079/1‑57 (MQ=255)
                                                        aCCACTGGCAGAAATATAGGCATAGGTCAGtatata                                                <  1:3513560/36‑1 (MQ=255)
                                                        aCCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt             <  1:2963379/71‑1 (MQ=255)
                                                        aCCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt             <  1:1730270/71‑1 (MQ=255)
                                                         ccACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCa                                   >  1:2968278/1‑48 (MQ=255)
                                                         ccACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATg                                >  1:3844157/1‑51 (MQ=255)
                                                         ccACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGc                         >  1:1487521/1‑58 (MQ=255)
                                                          cACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACaaa                                       <  1:2793215/43‑1 (MQ=255)
                                                          cACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                     <  1:2286262/61‑1 (MQ=255)
                                                          cACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                     <  1:645678/61‑1 (MQ=255)
                                                             tGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAg        >  1:1530889/1‑71 (MQ=255)
                                                              ggCAGAAATATAGGCATAGGTCAGGATATAGAGCACaa                                        <  1:3259477/38‑1 (MQ=255)
                                                              ggCAGAAATATAGGCATAGGTCAGGATATAGAGCACaa                                        <  1:2447768/38‑1 (MQ=255)
                                                               gCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAggt      <  1:2258372/71‑3 (MQ=255)
                                                                cAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGataa                <  1:2207693/60‑4 (MQ=255)
                                                                cAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt     <  1:631903/71‑1 (MQ=255)
                                                                cAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt     <  1:3900250/71‑1 (MQ=255)
                                                                 aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                     <  1:315029/54‑1 (MQ=255)
                                                                 aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTc           <  1:1768982/64‑1 (MQ=255)
                                                                 aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCtt    <  1:3786149/71‑1 (MQ=255)
                                                                 aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt     <  1:476426/70‑1 (MQ=255)
                                                                   aaaTATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTg  <  1:2108386/71‑1 (MQ=255)
                                                                    |                                                                     
GCCGCCCGTGCCGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTG  >  minE/2130516‑2130653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: