| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,130,596 | G→A | 9.1% | T103T (ACC→ACT) | mtr ← | tryptophan transporter of high affinity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,130,596 | 0 | G | A | 9.1% | 223.7 / 9.7 | 78 | T103T (ACC→ACT) | mtr | tryptophan transporter of high affinity |
| Reads supporting (aligned to +/- strand): ref base G (28/42); new base A (5/2); total (33/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01 | |||||||||||
ACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTT > minE/2130531‑2130666 | aCGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAg < 1:1396721/66‑1 (MQ=255) tAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGata > 1:52062/1‑70 (MQ=255) gTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGata > 1:3497767/1‑68 (MQ=255) gACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATagag > 1:2327900/1‑71 (MQ=255) aCATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATagag > 1:238406/1‑70 (MQ=255) cATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca < 1:829437/71‑1 (MQ=255) aTCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGt < 1:2843739/56‑1 (MQ=255) aTCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca > 1:2079159/1‑70 (MQ=255) ctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCa > 1:2219800/1‑54 (MQ=255) tGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGcaca < 1:3570034/67‑1 (MQ=255) tGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGcaca < 1:1317160/67‑1 (MQ=255) tGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGcaca < 1:2026017/67‑1 (MQ=255) gAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGGCAGGATATaga < 1:2413942/59‑1 (MQ=255) ggTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATg > 1:2699596/1‑70 (MQ=255) gtgtGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATgg > 1:1830348/1‑70 (MQ=255) gtgATTCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCa < 1:974314/44‑1 (MQ=255) gtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACaa < 1:1000448/59‑1 (MQ=255) tgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCaa > 1:1259575/1‑64 (MQ=255) tgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAAt > 1:816929/1‑71 (MQ=255) tgAAGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCa > 1:1606479/1‑63 (MQ=255) gATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGt < 1:1155305/40‑1 (MQ=255) gATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAg < 1:901283/43‑1 (MQ=255) aTGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGc > 1:3756300/1‑71 (MQ=255) cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGa > 1:1911112/1‑41 (MQ=255) cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACaa > 1:1656897/1‑53 (MQ=255) cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCg < 1:3419028/70‑1 (MQ=255) cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCg < 1:313305/70‑1 (MQ=255) cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCg < 1:2476653/70‑1 (MQ=255) aGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt > 1:3841630/1‑71 (MQ=255) gAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt > 1:3882045/1‑70 (MQ=255) gAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTg < 1:3419420/71‑1 (MQ=255) aaTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCa < 1:2064659/55‑1 (MQ=255) aaTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCa < 1:1998809/55‑1 (MQ=255) aTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGa > 1:2638131/1‑70 (MQ=255) aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGt < 1:220924/67‑1 (MQ=255) aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGt < 1:2570390/67‑1 (MQ=255) tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAAt > 1:615111/1‑55 (MQ=255) tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc > 1:1333389/1‑70 (MQ=255) tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc > 1:2752857/1‑70 (MQ=255) cGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGaa < 1:2238363/58‑1 (MQ=255) gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCAc > 1:431476/1‑71 (MQ=255) gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCAc > 1:3038275/1‑71 (MQ=255) caCCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAAta > 1:2801671/2‑58 (MQ=255) caCCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAAta > 1:578192/2‑58 (MQ=255) aacaacTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATgg < 1:2188696/54‑1 (MQ=255) aCCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACgaa > 1:3037448/1‑45 (MQ=255) aCCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAAtac > 1:2706079/1‑57 (MQ=255) aCCACTGGCAGAAATATAGGCATAGGTCAGtatata < 1:3513560/36‑1 (MQ=255) aCCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt < 1:1730270/71‑1 (MQ=255) aCCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt < 1:2963379/71‑1 (MQ=255) ccACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCa > 1:2968278/1‑48 (MQ=255) ccACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATg > 1:3844157/1‑51 (MQ=255) ccACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGc > 1:1487521/1‑58 (MQ=255) cACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACaaa < 1:2793215/43‑1 (MQ=255) cACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt < 1:2286262/61‑1 (MQ=255) cACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt < 1:645678/61‑1 (MQ=255) tGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAg > 1:1530889/1‑71 (MQ=255) ggCAGAAATATAGGCATAGGTCAGGATATAGAGCACaa < 1:3259477/38‑1 (MQ=255) ggCAGAAATATAGGCATAGGTCAGGATATAGAGCACaa < 1:2447768/38‑1 (MQ=255) gCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAggt < 1:2258372/71‑3 (MQ=255) cAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGataa < 1:2207693/60‑4 (MQ=255) cAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt < 1:631903/71‑1 (MQ=255) cAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt < 1:3900250/71‑1 (MQ=255) aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt < 1:315029/54‑1 (MQ=255) aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTc < 1:1768982/64‑1 (MQ=255) aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCtt < 1:3786149/71‑1 (MQ=255) aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt < 1:476426/70‑1 (MQ=255) aaaTATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTg < 1:2108386/71‑1 (MQ=255) tataGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc < 1:1300912/70‑1 (MQ=255) ataGGCATAGGTCAGGATATAGAGCACAAAGGCAATgg < 1:1976668/38‑1 (MQ=255) taGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCa < 1:205213/70‑1 (MQ=255) aGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTg < 1:159205/47‑1 (MQ=255) ggCATACGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt < 1:267439/45‑1 (MQ=255) gCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaa < 1:2968351/71‑1 (MQ=255) aTAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGc < 1:3927449/67‑1 (MQ=255) aTAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt < 1:572894/71‑1 (MQ=255) tAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc > 1:79700/1‑71 (MQ=255) aGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt > 1:342043/1‑71 (MQ=255) aGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt > 1:680087/1‑71 (MQ=255) ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt < 1:2775292/71‑1 (MQ=255) ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt < 1:1548219/70‑1 (MQ=255) | ACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTT > minE/2130531‑2130666 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |