| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,130,611 | C→A | 11.6% | V98V (GTG→GTT) | mtr ← | tryptophan transporter of high affinity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,130,611 | 0 | C | A | 11.6% | 266.4 / 12.5 | 86 | V98V (GTG→GTT) | mtr | tryptophan transporter of high affinity |
| Reads supporting (aligned to +/- strand): ref base C (26/50); new base A (7/3); total (33/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.98e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
CATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAA > minE/2130542‑2130681 | cATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca < 1:829437/71‑1 (MQ=255) aTCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca > 1:2079159/1‑70 (MQ=255) tGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGcaca < 1:1317160/67‑1 (MQ=255) tGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGcaca < 1:2026017/67‑1 (MQ=255) tGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGcaca < 1:3570034/67‑1 (MQ=255) ggTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATg > 1:2699596/1‑70 (MQ=255) gtgtGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATgg > 1:1830348/1‑70 (MQ=255) gtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACaa < 1:1000448/59‑1 (MQ=255) tgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCaa > 1:1259575/1‑64 (MQ=255) tgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAAt > 1:816929/1‑71 (MQ=255) tgAAGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCa > 1:1606479/1‑63 (MQ=255) aTGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGc > 1:3756300/1‑71 (MQ=255) cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACaa > 1:1656897/1‑53 (MQ=255) cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCg < 1:2476653/70‑1 (MQ=255) cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCg < 1:313305/70‑1 (MQ=255) cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCg < 1:3419028/70‑1 (MQ=255) aGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt > 1:3841630/1‑71 (MQ=255) gAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt > 1:3882045/1‑70 (MQ=255) gAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTg < 1:3419420/71‑1 (MQ=255) aaTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCa < 1:2064659/55‑1 (MQ=255) aaTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCa < 1:1998809/55‑1 (MQ=255) aTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGa > 1:2638131/1‑70 (MQ=255) aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGt < 1:2570390/67‑1 (MQ=255) aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGt < 1:220924/67‑1 (MQ=255) tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAAt > 1:615111/1‑55 (MQ=255) tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc > 1:2752857/1‑70 (MQ=255) tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc > 1:1333389/1‑70 (MQ=255) cGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGaa < 1:2238363/58‑1 (MQ=255) gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCAc > 1:431476/1‑71 (MQ=255) gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCAc > 1:3038275/1‑71 (MQ=255) caCCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAAta > 1:2801671/2‑58 (MQ=255) caCCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAAta > 1:578192/2‑58 (MQ=255) aacaacTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATgg < 1:2188696/54‑1 (MQ=255) aCCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACgaa > 1:3037448/1‑45 (MQ=255) aCCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAAtac > 1:2706079/1‑57 (MQ=255) aCCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt < 1:1730270/71‑1 (MQ=255) aCCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt < 1:2963379/71‑1 (MQ=255) ccACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCa > 1:2968278/1‑48 (MQ=255) ccACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATg > 1:3844157/1‑51 (MQ=255) ccACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGc > 1:1487521/1‑58 (MQ=255) cACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACaaa < 1:2793215/43‑1 (MQ=255) cACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt < 1:2286262/61‑1 (MQ=255) cACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt < 1:645678/61‑1 (MQ=255) tGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAg > 1:1530889/1‑71 (MQ=255) ggCAGAAATATAGGCATAGGTCAGGATATAGAGCACaa < 1:3259477/38‑1 (MQ=255) ggCAGAAATATAGGCATAGGTCAGGATATAGAGCACaa < 1:2447768/38‑1 (MQ=255) gCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAggt < 1:2258372/71‑3 (MQ=255) cAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGataa < 1:2207693/60‑4 (MQ=255) cAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt < 1:631903/71‑1 (MQ=255) cAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt < 1:3900250/71‑1 (MQ=255) aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt < 1:315029/54‑1 (MQ=255) aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTc < 1:1768982/64‑1 (MQ=255) aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCtt < 1:3786149/71‑1 (MQ=255) aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt < 1:476426/70‑1 (MQ=255) aaaTATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTg < 1:2108386/71‑1 (MQ=255) tataGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc < 1:1300912/70‑1 (MQ=255) ataGGCATAGGTCAGGATATAGAGCACAAAGGCAATgg < 1:1976668/38‑1 (MQ=255) taGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCa < 1:205213/70‑1 (MQ=255) aGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTg < 1:159205/47‑1 (MQ=255) ggCATACGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt < 1:267439/45‑1 (MQ=255) gCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaa < 1:2968351/71‑1 (MQ=255) aTAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGc < 1:3927449/67‑1 (MQ=255) aTAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt < 1:572894/71‑1 (MQ=255) tAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc > 1:79700/1‑71 (MQ=255) aGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt > 1:342043/1‑71 (MQ=255) aGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt > 1:680087/1‑71 (MQ=255) ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt < 1:2775292/71‑1 (MQ=255) ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt < 1:1548219/70‑1 (MQ=255) gTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt < 1:2512851/71‑1 (MQ=255) gTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt < 1:2810825/71‑1 (MQ=255) tCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg < 1:2372374/71‑1 (MQ=255) aGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc < 1:2156590/49‑1 (MQ=255) aGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc < 1:596894/49‑1 (MQ=255) aGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc < 1:2374901/49‑1 (MQ=255) gtatatatAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaaa < 1:769920/60‑1 (MQ=255) gATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc < 1:1485245/47‑1 (MQ=255) atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt > 1:3774950/1‑71 (MQ=255) atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt > 1:1591118/1‑71 (MQ=255) atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt < 1:3234720/71‑1 (MQ=255) tataGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaa < 1:3151871/58‑1 (MQ=255) ataGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATgg > 1:2353629/1‑71 (MQ=255) ataGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATgg > 1:398382/1‑71 (MQ=255) taGAGCACTAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt < 1:177020/71‑1 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATgg < 1:3634079/70‑1 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt > 1:1700784/1‑71 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt < 1:2526857/71‑1 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt > 1:1037977/1‑71 (MQ=255) taGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGgt < 1:1728274/71‑1 (MQ=255) agagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt > 1:378976/1‑70 (MQ=255) gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgtg < 1:2954836/70‑1 (MQ=255) gCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTc < 1:2107454/70‑1 (MQ=255) cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt < 1:58497/63‑1 (MQ=255) cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:507759/71‑1 (MQ=255) cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:3182582/71‑1 (MQ=255) cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:1773326/71‑1 (MQ=255) | CATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAA > minE/2130542‑2130681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |