Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,130,633 T→C 10.5% N91S (AAC→AGC)  mtr ← tryptophan transporter of high affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,130,6330TC10.5% 255.7 / 17.8 95N91S (AAC→AGC) mtrtryptophan transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base T (37/48);  new base C (6/4);  total (43/52)
Fisher's exact test for biased strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 105 total aligned reads displayed.

AGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATT  >  minE/2130564‑2130703
                                                                     |                                                                      
aGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                                                                       >  1:3841630/1‑71 (MQ=255)
 gAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTg                                                                      <  1:3419420/71‑1 (MQ=255)
   aTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGa                                                                     >  1:2638131/1‑70 (MQ=255)
   aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGt                                                                        <  1:2570390/67‑1 (MQ=255)
   aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGt                                                                        <  1:220924/67‑1 (MQ=255)
    tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc                                                                    >  1:2752857/1‑70 (MQ=255)
    tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc                                                                    >  1:1333389/1‑70 (MQ=255)
      gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCAc                                                                 >  1:431476/1‑71 (MQ=255)
      gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCAc                                                                 >  1:3038275/1‑71 (MQ=255)
        aCCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt                                                               <  1:1730270/71‑1 (MQ=255)
        aCCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt                                                               <  1:2963379/71‑1 (MQ=255)
          cACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                                                                       <  1:645678/61‑1 (MQ=255)
          cACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                                                                       <  1:2286262/61‑1 (MQ=255)
             tGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAg                                                          >  1:1530889/1‑71 (MQ=255)
               gCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAggt                                                        <  1:2258372/71‑3 (MQ=255)
                cAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGataa                                                                  <  1:2207693/60‑4 (MQ=255)
                cAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt                                                       <  1:3900250/71‑1 (MQ=255)
                cAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt                                                       <  1:631903/71‑1 (MQ=255)
                 aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                                                                       <  1:315029/54‑1 (MQ=255)
                 aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTc                                                             <  1:1768982/64‑1 (MQ=255)
                 aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCtt                                                      <  1:3786149/71‑1 (MQ=255)
                 aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt                                                       <  1:476426/70‑1 (MQ=255)
                   aaaTATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTg                                                    <  1:2108386/71‑1 (MQ=255)
                      tataGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc                                                  <  1:1300912/70‑1 (MQ=255)
                        taGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCa                                                <  1:205213/70‑1 (MQ=255)
                         aGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTg                                                                      <  1:159205/47‑1 (MQ=255)
                          ggCATACGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                                                                       <  1:267439/45‑1 (MQ=255)
                           gCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaa                                            <  1:2968351/71‑1 (MQ=255)
                             aTAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGc                                              <  1:3927449/67‑1 (MQ=255)
                             aTAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt                                          <  1:572894/71‑1 (MQ=255)
                              tAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc                                         >  1:79700/1‑71 (MQ=255)
                               aGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt                                        >  1:680087/1‑71 (MQ=255)
                               aGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt                                        >  1:342043/1‑71 (MQ=255)
                                ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt                                       <  1:2775292/71‑1 (MQ=255)
                                ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt                                        <  1:1548219/70‑1 (MQ=255)
                                 gTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt                                      <  1:2512851/71‑1 (MQ=255)
                                 gTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt                                      <  1:2810825/71‑1 (MQ=255)
                                  tCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg                                     <  1:2372374/71‑1 (MQ=255)
                                    aGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc                                                         <  1:596894/49‑1 (MQ=255)
                                    aGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc                                                         <  1:2156590/49‑1 (MQ=255)
                                     gtatatatAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaaa                                           <  1:769920/60‑1 (MQ=255)
                                      gATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc                                                         <  1:1485245/47‑1 (MQ=255)
                                       atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt                                >  1:3774950/1‑71 (MQ=255)
                                       atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt                                <  1:3234720/71‑1 (MQ=255)
                                       atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt                                >  1:1591118/1‑71 (MQ=255)
                                        tataGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaa                                            <  1:3151871/58‑1 (MQ=255)
                                         ataGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATgg                              >  1:2353629/1‑71 (MQ=255)
                                         ataGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATgg                              >  1:398382/1‑71 (MQ=255)
                                          taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATgg                              <  1:3634079/70‑1 (MQ=255)
                                          taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt                             >  1:1037977/1‑71 (MQ=255)
                                          taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt                             <  1:2526857/71‑1 (MQ=255)
                                          taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt                             >  1:1700784/1‑71 (MQ=255)
                                          taGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGgt                             <  1:1728274/71‑1 (MQ=255)
                                           agagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt                             >  1:378976/1‑70 (MQ=255)
                                            gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgtg                            <  1:2954836/70‑1 (MQ=255)
                                              gCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTc                          <  1:2107454/70‑1 (MQ=255)
                                               cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt                                <  1:58497/63‑1 (MQ=255)
                                               cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa                        <  1:3182582/71‑1 (MQ=255)
                                               cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa                        <  1:507759/71‑1 (MQ=255)
                                               cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa                        <  1:1773326/71‑1 (MQ=255)
                                                acgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaa                        >  1:2643147/4‑70 (MQ=255)
                                                acgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaa                        >  1:620774/4‑70 (MQ=255)
                                                acaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa                        >  1:3648120/1‑70 (MQ=255)
                                                acaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa                        <  1:2348795/70‑1 (MQ=255)
                                                 caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt                                      >  1:2175401/1‑55 (MQ=255)
                                                 caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt                                      >  1:3412681/1‑55 (MQ=255)
                                                 caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaaaa                      <  1:1876149/71‑1 (MQ=255)
                                                 caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaaaa                      >  1:369498/1‑71 (MQ=255)
                                                   aaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg                                     >  1:1053184/1‑54 (MQ=255)
                                                   aaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg                                     >  1:3254630/1‑54 (MQ=255)
                                                   aaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACt                    <  1:2386780/71‑1 (MQ=255)
                                                    aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTc                   >  1:3152333/1‑71 (MQ=255)
                                                    aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTc                   <  1:2069571/71‑1 (MQ=255)
                                                    aGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTAAAACtg                   >  1:643712/1‑70 (MQ=255)
                                                      gCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt                                       <  1:837689/49‑1 (MQ=255)
                                                      gCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGa                 <  1:1154618/71‑1 (MQ=255)
                                                        aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg                                     >  1:1236744/1‑49 (MQ=255)
                                                         aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt                                   <  1:1991982/50‑1 (MQ=255)
                                                         aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa                        <  1:467502/61‑1 (MQ=255)
                                                         aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc              >  1:1096749/1‑71 (MQ=255)
                                                         aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc              >  1:2725876/1‑71 (MQ=255)
                                                         aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc              <  1:2346839/71‑1 (MQ=255)
                                                         aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc              <  1:1703473/71‑1 (MQ=255)
                                                         aTGGAAATGACGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc              >  1:787359/1‑71 (MQ=255)
                                                          tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACt                    >  1:2888344/1‑64 (MQ=255)
                                                          tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACt                    >  1:696586/1‑64 (MQ=255)
                                                          tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc              >  1:2521322/1‑70 (MQ=255)
                                                           ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgtgt                           >  1:3820079/1‑56 (MQ=255)
                                                           ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGa            >  1:3016953/1‑71 (MQ=255)
                                                            gAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt                                          >  1:2238315/1‑40 (MQ=255)
                                                            gAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt                                          >  1:1531108/1‑40 (MQ=255)
                                                            gAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACt                    >  1:3011779/1‑62 (MQ=255)
                                                                 gCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGaa                >  1:2631467/1‑61 (MQ=255)
                                                                  ccGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTc                   <  1:2119005/57‑1 (MQ=255)
                                                                  ccGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGt      >  1:3055926/1‑70 (MQ=255)
                                                                   cGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTg       <  1:3256697/68‑1 (MQ=255)
                                                                   cGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTaa    >  1:188991/1‑71 (MQ=255)
                                                                   cGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTaa    >  1:1615672/1‑71 (MQ=255)
                                                                     ttGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAAtt  <  1:1675423/71‑1 (MQ=255)
                                                                     ttGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAAtt  >  1:2915736/1‑71 (MQ=255)
                                                                     |                                                                      
AGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATT  >  minE/2130564‑2130703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: