| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,130,638 | C→A | 8.4% | V89V (GTG→GTT) ‡ | mtr ← | tryptophan transporter of high affinity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,130,638 | 0 | C | A | 8.4% | 305.3 / 7.9 | 94 | V89V (GTG→GTT) ‡ | mtr | tryptophan transporter of high affinity |
| Reads supporting (aligned to +/- strand): ref base C (38/48); new base A (5/3); total (43/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01 | |||||||||||
GAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGG > minE/2130570‑2130707 | gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCAc > 1:431476/1‑71 (MQ=255)gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCAc > 1:3038275/1‑71 (MQ=255) aCCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt < 1:1730270/71‑1 (MQ=255) aCCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt < 1:2963379/71‑1 (MQ=255) tGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAg > 1:1530889/1‑71 (MQ=255) gCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAggt < 1:2258372/71‑3 (MQ=255) cAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt < 1:3900250/71‑1 (MQ=255) cAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt < 1:631903/71‑1 (MQ=255) aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTc < 1:1768982/64‑1 (MQ=255) aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCtt < 1:3786149/71‑1 (MQ=255) aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt < 1:476426/70‑1 (MQ=255) aaaTATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTg < 1:2108386/71‑1 (MQ=255) tataGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc < 1:1300912/70‑1 (MQ=255) taGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCa < 1:205213/70‑1 (MQ=255) gCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaa < 1:2968351/71‑1 (MQ=255) aTAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGc < 1:3927449/67‑1 (MQ=255) aTAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt < 1:572894/71‑1 (MQ=255) tAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc > 1:79700/1‑71 (MQ=255) aGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt > 1:342043/1‑71 (MQ=255) aGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt > 1:680087/1‑71 (MQ=255) ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt < 1:2775292/71‑1 (MQ=255) ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt < 1:1548219/70‑1 (MQ=255) gTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt < 1:2810825/71‑1 (MQ=255) gTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt < 1:2512851/71‑1 (MQ=255) tCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg < 1:2372374/71‑1 (MQ=255) aGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc < 1:596894/49‑1 (MQ=255) aGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc < 1:2374901/49‑1 (MQ=255) aGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc < 1:2156590/49‑1 (MQ=255) gtatatatAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaaa < 1:769920/60‑1 (MQ=255) gATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc < 1:1485245/47‑1 (MQ=255) atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt < 1:3234720/71‑1 (MQ=255) atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt > 1:3774950/1‑71 (MQ=255) atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt > 1:1591118/1‑71 (MQ=255) tataGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaa < 1:3151871/58‑1 (MQ=255) ataGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATgg > 1:398382/1‑71 (MQ=255) ataGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATgg > 1:2353629/1‑71 (MQ=255) taGAGCACTAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt < 1:177020/71‑1 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATgg < 1:3634079/70‑1 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt < 1:2526857/71‑1 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt > 1:1037977/1‑71 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt > 1:1700784/1‑71 (MQ=255) taGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGgt < 1:1728274/71‑1 (MQ=255) agagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt > 1:378976/1‑70 (MQ=255) gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgtg < 1:2954836/70‑1 (MQ=255) gCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTc < 1:2107454/70‑1 (MQ=255) cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt < 1:58497/63‑1 (MQ=255) cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:1773326/71‑1 (MQ=255) cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:507759/71‑1 (MQ=255) cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:3182582/71‑1 (MQ=255) acgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaa > 1:2643147/4‑70 (MQ=255) acgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaa > 1:620774/4‑70 (MQ=255) acaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa > 1:3648120/1‑70 (MQ=255) acaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:2348795/70‑1 (MQ=255) caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt > 1:2175401/1‑55 (MQ=255) caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt > 1:3412681/1‑55 (MQ=255) caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaaaa < 1:1876149/71‑1 (MQ=255) caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaaaa > 1:369498/1‑71 (MQ=255) gaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGTTGGTGTTaaaa < 1:3035046/69‑1 (MQ=255) aaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg > 1:3254630/1‑54 (MQ=255) aaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg > 1:1053184/1‑54 (MQ=255) aaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACt < 1:2386780/71‑1 (MQ=255) aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTc > 1:3152333/1‑71 (MQ=255) aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTc < 1:2069571/71‑1 (MQ=255) aGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTAAAACtg > 1:643712/1‑70 (MQ=255) gCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt < 1:837689/49‑1 (MQ=255) gCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGa < 1:1154618/71‑1 (MQ=255) aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg > 1:1236744/1‑49 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt < 1:1991982/50‑1 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:467502/61‑1 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc < 1:2346839/71‑1 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc < 1:1703473/71‑1 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc > 1:2725876/1‑71 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc > 1:1096749/1‑71 (MQ=255) aTGGAAATGACGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc > 1:787359/1‑71 (MQ=255) tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACt > 1:696586/1‑64 (MQ=255) tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACt > 1:2888344/1‑64 (MQ=255) tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc > 1:2521322/1‑70 (MQ=255) ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgtgt > 1:3820079/1‑56 (MQ=255) ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGa > 1:3016953/1‑71 (MQ=255) gAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt > 1:2238315/1‑40 (MQ=255) gAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt > 1:1531108/1‑40 (MQ=255) gAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACt > 1:889928/1‑62 (MQ=255) gAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACt > 1:3011779/1‑62 (MQ=255) gCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGaa > 1:2631467/1‑61 (MQ=255) ccGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTc < 1:2119005/57‑1 (MQ=255) ccGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGt > 1:3055926/1‑70 (MQ=255) cGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTg < 1:3256697/68‑1 (MQ=255) cGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTaa > 1:1615672/1‑71 (MQ=255) cGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTaa > 1:188991/1‑71 (MQ=255) ttGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAAtt < 1:1675423/71‑1 (MQ=255) ttGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAAtt > 1:2915736/1‑71 (MQ=255) tGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAAtt > 1:1418809/1‑70 (MQ=255) tGACCACGTTCCAGCCTTTGCCCAGCAAATC‑TTCGTGATGGTGTCAAAACTCGAACCGATTCTGTAAtt > 1:86946/1‑69 (MQ=39) aCCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAg < 1:1114843/71‑1 (MQ=255) ccACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAgg < 1:570598/71‑1 (MQ=255) | GAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGG > minE/2130570‑2130707 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |