| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,130,650 | T→G | 7.2% | K85N (AAA→AAC) | mtr ← | tryptophan transporter of high affinity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,130,650 | 0 | T | G | 7.2% | 247.6 / 13.0 | 97 | K85N (AAA→AAC) | mtr | tryptophan transporter of high affinity |
| Reads supporting (aligned to +/- strand): ref base T (45/45); new base G (5/2); total (50/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
CAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAA > minE/2130580‑2130720 | cAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt < 1:631903/71‑1 (MQ=255)cAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt < 1:3900250/71‑1 (MQ=255) aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCtt < 1:3786149/71‑1 (MQ=255) aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt < 1:476426/70‑1 (MQ=255) aaaTATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTg < 1:2108386/71‑1 (MQ=255) tataGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc < 1:1300912/70‑1 (MQ=255) taGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCa < 1:205213/70‑1 (MQ=255) gCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaa < 1:2968351/71‑1 (MQ=255) aTAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGc < 1:3927449/67‑1 (MQ=255) aTAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt < 1:572894/71‑1 (MQ=255) tAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc > 1:79700/1‑71 (MQ=255) aGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt > 1:680087/1‑71 (MQ=255) aGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt > 1:342043/1‑71 (MQ=255) ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt < 1:2775292/71‑1 (MQ=255) ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt < 1:1548219/70‑1 (MQ=255) gTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt < 1:2810825/71‑1 (MQ=255) gTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt < 1:2512851/71‑1 (MQ=255) tCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg < 1:2372374/71‑1 (MQ=255) gtatatatAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaaa < 1:769920/60‑1 (MQ=255) atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt < 1:3234720/71‑1 (MQ=255) atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt > 1:3774950/1‑71 (MQ=255) atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt > 1:1591118/1‑71 (MQ=255) tataGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaa < 1:3151871/58‑1 (MQ=255) ataGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATgg > 1:398382/1‑71 (MQ=255) ataGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATgg > 1:2353629/1‑71 (MQ=255) taGAGCACTAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt < 1:177020/71‑1 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATgg < 1:3634079/70‑1 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt > 1:1037977/1‑71 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt < 1:2526857/71‑1 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt > 1:1700784/1‑71 (MQ=255) taGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGgt < 1:1728274/71‑1 (MQ=255) agagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt > 1:378976/1‑70 (MQ=255) gCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTc < 1:2107454/70‑1 (MQ=255) cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt < 1:58497/63‑1 (MQ=255) cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:1773326/71‑1 (MQ=255) cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:3182582/71‑1 (MQ=255) cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:507759/71‑1 (MQ=255) acgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaa > 1:2643147/4‑70 (MQ=255) acgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaa > 1:620774/4‑70 (MQ=255) acaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:2348795/70‑1 (MQ=255) acaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa > 1:3648120/1‑70 (MQ=255) caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt > 1:3412681/1‑55 (MQ=255) caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt > 1:2175401/1‑55 (MQ=255) caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaaaa > 1:369498/1‑71 (MQ=255) caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaaaa < 1:1876149/71‑1 (MQ=255) gaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGTTGGTGTTaaaa < 1:3035046/69‑1 (MQ=255) aaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg > 1:3254630/1‑54 (MQ=255) aaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg > 1:1053184/1‑54 (MQ=255) aaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACt < 1:2386780/71‑1 (MQ=255) aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTc < 1:2069571/71‑1 (MQ=255) aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTc > 1:3152333/1‑71 (MQ=255) aGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTAAAACtg > 1:643712/1‑70 (MQ=255) gCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt < 1:837689/49‑1 (MQ=255) gCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGa < 1:1154618/71‑1 (MQ=255) aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg > 1:1236744/1‑49 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:467502/61‑1 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc < 1:2346839/71‑1 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc > 1:1096749/1‑71 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc > 1:2725876/1‑71 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc < 1:1703473/71‑1 (MQ=255) aTGGAAATGACGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc > 1:787359/1‑71 (MQ=255) tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACt > 1:2888344/1‑64 (MQ=255) tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACt > 1:696586/1‑64 (MQ=255) tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc > 1:2521322/1‑70 (MQ=255) ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgtgt > 1:3820079/1‑56 (MQ=255) ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGa > 1:3016953/1‑71 (MQ=255) gAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt > 1:2238315/1‑40 (MQ=255) gAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt > 1:1531108/1‑40 (MQ=255) gAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACt > 1:3011779/1‑62 (MQ=255) gCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGaa > 1:2631467/1‑61 (MQ=255) ccGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTc < 1:2119005/57‑1 (MQ=255) ccGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGt > 1:3055926/1‑70 (MQ=255) cGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTg < 1:3256697/68‑1 (MQ=255) cGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTaa > 1:1615672/1‑71 (MQ=255) cGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTaa > 1:188991/1‑71 (MQ=255) ttGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAAtt < 1:1675423/71‑1 (MQ=255) ttGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAAtt > 1:2915736/1‑71 (MQ=255) tGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAAtt > 1:1418809/1‑70 (MQ=255) tGACCACGTTCCAGCCTTTGCCCAGCAAATC‑TTCGTGATGGTGTCAAAACTCGAACCGATTCTGTAAtt > 1:86946/1‑69 (MQ=39) aCCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAg < 1:1114843/71‑1 (MQ=255) ccACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAgg < 1:570598/71‑1 (MQ=255) aCGTTCCAGCCTTTGCCCAGCAAATCTTAGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCACGt > 1:3297985/1‑70 (MQ=255) cGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGa < 1:3722550/54‑1 (MQ=255) cGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTaa > 1:3629755/1‑62 (MQ=255) cGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGt > 1:683287/1‑69 (MQ=255) ttCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGc < 1:2760230/71‑1 (MQ=255) ttCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGc > 1:1519517/1‑71 (MQ=255) ccAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCtt > 1:3097267/1‑71 (MQ=255) ccAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCt < 1:200495/70‑1 (MQ=255) cAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCa > 1:3736095/1‑61 (MQ=255) cAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTc < 1:3153347/71‑1 (MQ=255) aGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCg < 1:1587678/42‑1 (MQ=255) aGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTc > 1:369586/1‑70 (MQ=255) gCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGaa > 1:1707710/1‑43 (MQ=255) ccTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTc > 1:713591/1‑68 (MQ=255) ccTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAg < 1:1345481/71‑1 (MQ=255) cTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGa < 1:3330457/71‑1 (MQ=255) tttGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGaa > 1:1578122/1‑71 (MQ=255) tttGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGaa > 1:2612015/1‑71 (MQ=255) tttGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTAAGGTTAGCTTCCAGa > 1:33001/1‑70 (MQ=255) | CAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAA > minE/2130580‑2130720 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |