| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,131,666 | G→A | 19.3% | L428L (CTG→TTG) | deaD ← | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,131,666 | 0 | G | A | 19.3% | 133.4 / 24.9 | 62 | L428L (CTG→TTG) | deaD | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (29/21); new base A (5/7); total (34/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTGC > minE/2131597‑2131735 | cACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGt > 1:2255154/1‑71 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGcc > 1:38507/1‑71 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGcc > 1:1966840/1‑71 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGcc > 1:3728700/1‑71 (MQ=255) aCGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCa < 1:3902688/71‑1 (MQ=255) aTCGGCTCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGt > 1:847/1‑71 (MQ=255) aTCGGCGCATCTGGCGGTACGTTCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGt > 1:94822/1‑57 (MQ=255) aTCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGt > 1:2289604/1‑57 (MQ=255) aTCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGt > 1:1196590/1‑71 (MQ=255) aTCGGCGCATCTGGCGGTACGAT‑AGAGTACGTTCACCCTGTGCCATTTTCAGCAGt > 1:2600128/1‑56 (MQ=255) tCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGtt < 1:2931115/71‑1 (MQ=255) tCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGtt < 1:2244944/71‑1 (MQ=255) tCGGCCCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGtt < 1:940071/71‑1 (MQ=255) cATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCgaga < 1:545980/71‑1 (MQ=255) cATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCgaga < 1:1509129/71‑1 (MQ=255) aTCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTc < 1:3214255/66‑1 (MQ=255) aTCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTc > 1:3825005/1‑66 (MQ=255) tCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGt < 1:3091685/48‑1 (MQ=255) tGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTcagcag > 1:2992617/1‑45 (MQ=255) tGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTcagcag > 1:680809/1‑45 (MQ=255) ggCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAg > 1:3870425/1‑71 (MQ=255) gCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATcc < 1:167322/68‑1 (MQ=255) gCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATcc < 1:2057070/68‑1 (MQ=255) gTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctct < 1:589651/71‑1 (MQ=255) gTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctct < 1:2962949/71‑1 (MQ=255) gTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctct > 1:2635340/1‑71 (MQ=255) gTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctct > 1:3519060/1‑71 (MQ=255) aCGATCAGAGTACGTTCACCCTGTGCCATTTTcagcag > 1:273539/1‑38 (MQ=255) aCGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCtt > 1:3315015/1‑70 (MQ=255) aCGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCtt > 1:2604645/1‑70 (MQ=255) gATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCg > 1:3614054/1‑55 (MQ=255) aTCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCAcc < 1:3035699/71‑1 (MQ=255) cAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGAt > 1:2467453/1‑56 (MQ=255) cAGAGTACGTTCACCCTGTGCCATATTCAGCAGTGCCGCAGCCAGAGTTTCgag < 1:3666902/54‑1 (MQ=255) gagTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTc < 1:1157744/70‑1 (MQ=255) agTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTcag > 1:2554413/1‑71 (MQ=255) tACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGt < 1:1838530/43‑1 (MQ=255) tACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTcagca < 1:3132068/71‑1 (MQ=255) aCGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGc > 1:1838571/1‑36 (MQ=255) cGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGt > 1:698148/1‑71 (MQ=255) gTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTc < 1:2602995/71‑1 (MQ=255) ttCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCg < 1:1195112/71‑1 (MQ=255) cccTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTc < 1:1052183/38‑1 (MQ=255) ccTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGttt < 1:3946300/36‑1 (MQ=255) acTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTc > 1:1936170/2‑58 (MQ=255) tgtgCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGAt > 1:3317491/1‑40 (MQ=255) tgtgCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCgg > 1:3637630/1‑65 (MQ=255) tgCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCa < 1:2325357/49‑1 (MQ=255) tgCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCt > 1:1952655/1‑65 (MQ=255) tgCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAt < 1:1594290/69‑1 (MQ=255) ccATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTg < 1:1297583/71‑1 (MQ=255) cATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGc < 1:3939210/71‑1 (MQ=255) aTTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGCTCCAGCTCTTCAcc > 1:3445668/1‑47 (MQ=255) ttttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCt > 1:287203/1‑70 (MQ=255) tttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCtt > 1:822317/1‑41 (MQ=255) tttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCa > 1:3186932/1‑43 (MQ=255) tttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTcagc > 1:1004010/1‑51 (MQ=255) tttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAtt > 1:955529/1‑64 (MQ=255) ttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTcag > 1:220314/1‑71 (MQ=255) ttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTca < 1:3359401/70‑1 (MQ=255) ttCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCAc > 1:2269208/1‑43 (MQ=255) ttCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCAc > 1:3771663/1‑43 (MQ=255) tCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTcag < 1:2141248/70‑1 (MQ=255) tCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAACAGTCGGCTGAATTTTGCTcag > 1:3361875/1‑70 (MQ=255) tCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTcag < 1:2393026/70‑1 (MQ=255) agcagTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCa > 1:2879556/1‑39 (MQ=255) agcagTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTcagca < 1:1870572/48‑1 (MQ=255) agTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTgc < 1:3586104/71‑1 (MQ=255) | CACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTGC > minE/2131597‑2131735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |