| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,502 | G→T | 14.8% | A457A (GCC→GCA) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,502 | 0 | G | T | 14.8% | 221.8 / 16.5 | 74 | A457A (GCC→GCA) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (31/32); new base T (6/5); total (37/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
GCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCG > minE/311435‑311572 | gcgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGc < 1:3260098/70‑1 (MQ=255) cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCa < 1:2867743/71‑1 (MQ=255) gcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCAt < 1:3408709/71‑1 (MQ=255) gcCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCAt < 1:2113064/71‑1 (MQ=9) cATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCg > 1:2119889/1‑71 (MQ=255) aTTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg < 1:1450810/71‑1 (MQ=255) aTTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg < 1:3003775/71‑1 (MQ=255) ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg > 1:414223/1‑70 (MQ=255) ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg > 1:3866116/1‑70 (MQ=255) tGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa > 1:3122340/1‑71 (MQ=255) tGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCGTCggaa > 1:3936622/1‑69 (MQ=9) aaaCGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCAt > 1:2092038/1‑61 (MQ=9) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:2689047/68‑1 (MQ=255) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGaa > 1:574802/1‑70 (MQ=255) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAAt < 1:3545284/71‑1 (MQ=255) cAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAcc > 1:2666186/1‑71 (MQ=255) tGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgcc > 1:48657/1‑71 (MQ=255) tAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa > 1:3192846/1‑70 (MQ=255) tAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa > 1:2841052/1‑70 (MQ=255) tAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa > 1:702752/1‑70 (MQ=255) tAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa > 1:410310/1‑54 (MQ=255) agaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATa > 1:348051/1‑57 (MQ=255) agaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAcc < 1:2784490/71‑1 (MQ=255) gaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa > 1:960071/1‑71 (MQ=255) aCTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCAt < 1:880954/70‑1 (MQ=9) aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCAt < 1:2531350/70‑1 (MQ=255) aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATc < 1:2104716/71‑1 (MQ=255) aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATc < 1:3754158/71‑1 (MQ=255) aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCAGCGGTACGAATACCGCCGACAGTACCATc < 1:1121581/71‑1 (MQ=255) atGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcg > 1:1725338/2‑71 (MQ=255) tGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcg > 1:2672090/1‑70 (MQ=255) tGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGAACCAt < 1:1535646/68‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa > 1:1412848/1‑71 (MQ=255) gatagataGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATa < 1:1264754/71‑1 (MQ=255) gatagataGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATa > 1:2727115/1‑71 (MQ=255) tagatagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgcc < 1:443508/51‑1 (MQ=255) tagatagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATAcc < 1:812398/71‑1 (MQ=255) gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCag < 1:3266831/71‑1 (MQ=255) gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCag < 1:177690/71‑1 (MQ=255) gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa < 1:3768237/70‑1 (MQ=255) gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa < 1:3471492/70‑1 (MQ=255) gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa < 1:2442503/70‑1 (MQ=255) ccccAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCagt > 1:3500801/3‑70 (MQ=255) cACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGaa < 1:105259/37‑1 (MQ=255) cACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCaga < 1:136443/71‑1 (MQ=255) aCCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAg < 1:2882105/48‑1 (MQ=255) aCCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAg < 1:664219/48‑1 (MQ=255) aCCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAg < 1:266734/48‑1 (MQ=255) aCCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCagag < 1:2957357/71‑1 (MQ=255) aCCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCagag < 1:3841463/71‑1 (MQ=255) cAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATAc > 1:1743140/1‑60 (MQ=18) cAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgc > 1:3775808/1‑40 (MQ=255) cAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCaga > 1:2827285/1‑68 (MQ=255) cAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAACATCGCGATACCGACCAGAgc > 1:3517937/1‑70 (MQ=255) aGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCAc < 1:3759833/48‑1 (MQ=12) aGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTgcg > 1:3705393/1‑70 (MQ=255) aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAgcgc > 1:556739/1‑71 (MQ=255) gTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGcc > 1:2353725/1‑71 (MQ=255) tAGAGCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGt > 1:2681797/1‑44 (MQ=255) tAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGAt < 1:1074038/55‑1 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:3370656/1‑70 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:3637324/1‑70 (MQ=255) aaCCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGa < 1:3401176/71‑1 (MQ=255) aCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATa < 1:1926786/52‑1 (MQ=255) aCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGaa > 1:2304363/1‑71 (MQ=255) cGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAAtt < 1:3165198/71‑2 (MQ=255) cGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa > 1:1067930/1‑57 (MQ=255) gCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAAttt > 1:3818268/1‑69 (MQ=255) gCCAAAGAAGGCCATCGGTACTAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt < 1:3610260/71‑1 (MQ=255) gCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt < 1:2487857/71‑1 (MQ=255) cgaagaatGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATa > 1:250651/3‑47 (MQ=16) cgaagaatGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGc > 1:2403519/3‑71 (MQ=255) cAAAGAAGGCCATCGGTACGAATACCGCCGACACTACCATCGCGATACCGACCAGAGCGCCCTGa > 1:2256322/1‑65 (MQ=255) aaaGAAGGCCATCGGTACGAATACCGCCGACAGTACCAt > 1:2674628/1‑39 (MQ=255) aaaGAAGGCCATCGGTACGAATACCGCCGACAGTACCAt > 1:1177553/1‑39 (MQ=255) aaaGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGcc > 1:403912/1‑71 (MQ=255) aagaatGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATAccca > 1:228887/1‑47 (MQ=16) aagaagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGccc > 1:1450726/1‑71 (MQ=255) agaagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTg > 1:2517569/1‑61 (MQ=255) aagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCAt > 1:3391678/1‑71 (MQ=255) ggCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGaa > 1:2542684/1‑59 (MQ=255) ggCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCg < 1:2045518/71‑1 (MQ=255) | GCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCG > minE/311435‑311572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |