| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,155,591 | T→A | 9.7% | intergenic (+102/+84) | dacB → / ← obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,155,591 | 0 | T | A | 9.7% | 166.6 / 8.2 | 65 | intergenic (+102/+84) | dacB/obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Reads supporting (aligned to +/- strand): ref base T (38/19); new base A (4/2); total (43/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGT > minE/2155522‑2155661 | acAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc > 1:1908706/2‑71 (MQ=255) cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc < 1:2314111/70‑1 (MQ=255) cAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCg > 1:320434/1‑71 (MQ=255) cAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCg > 1:556950/1‑71 (MQ=255) gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg > 1:986703/1‑71 (MQ=255) ttgttGACAACGTACGCATTGTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAACAGTGCa < 1:3369653/68‑1 (MQ=1) ttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt > 1:653638/1‑71 (MQ=255) ttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCaaa < 1:2719167/70‑1 (MQ=255) tGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAAtt > 1:178360/1‑71 (MQ=255) aCAAATTGCGCTTTATGT‑TGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCaa > 1:1098698/1‑71 (MQ=11) aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt < 1:1707583/69‑1 (MQ=39) acaGTGCGCTTTGTTTATGCCAGATGCGGCGTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCaat < 1:3620551/69‑2 (MQ=11) aaaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa > 1:2252239/1‑70 (MQ=25) aaaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa > 1:2247584/1‑70 (MQ=25) aaaGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa > 1:1649628/1‑70 (MQ=35) aaaGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa > 1:1840022/1‑70 (MQ=35) gTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACata < 1:1502857/71‑1 (MQ=39) tGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACatat > 1:3163074/1‑71 (MQ=39) cgcTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTg > 1:592977/1‑71 (MQ=39) tttATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtc < 1:112347/70‑1 (MQ=255) tATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTc‑tct > 1:160016/1‑71 (MQ=255) ccGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAAt > 1:1427629/1‑47 (MQ=38) ccGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTa < 1:757168/71‑1 (MQ=255) cGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc < 1:3889732/48‑1 (MQ=255) ggATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAg < 1:2820287/70‑1 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAgg > 1:378758/1‑71 (MQ=11) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCt > 1:2547970/1‑71 (MQ=255) gcggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCt > 1:1473641/1‑70 (MQ=255) ggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt > 1:924232/1‑40 (MQ=40) ggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGa > 1:1435700/1‑70 (MQ=255) ggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGAt < 1:2525497/71‑1 (MQ=255) ggcgTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGa > 1:3172492/1‑71 (MQ=14) cgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTg < 1:204757/48‑1 (MQ=255) cgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATaa < 1:1881545/71‑1 (MQ=255) gTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc > 1:1065880/1‑38 (MQ=40) aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGc > 1:3349055/1‑60 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCg > 1:2966096/1‑70 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCg > 1:1649692/1‑70 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCg > 1:1818822/1‑70 (MQ=255) aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCCACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGtc > 1:900512/1‑70 (MQ=255) aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCg > 1:505943/1‑69 (MQ=255) ccTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAgcg > 1:1178601/1‑71 (MQ=255) cTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCaa > 1:634349/1‑42 (MQ=40) cTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCgca < 1:1723047/68‑3 (MQ=11) tATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:3104888/1‑71 (MQ=255) aTCCGGCCTACAAAATCGTGCAAATTCAACATATTGCTATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:828670/1‑70 (MQ=255) cGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAgg < 1:669121/71‑1 (MQ=255) gCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCt > 1:1363920/1‑71 (MQ=255) ccTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGc > 1:627270/1‑69 (MQ=255) ccTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCt > 1:1067304/1‑70 (MQ=255) ccTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCt > 1:1387815/1‑70 (MQ=255) cTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg < 1:2097864/40‑1 (MQ=255) cTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGcaa > 1:1006971/1‑69 (MQ=2) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGt > 1:2639210/1‑38 (MQ=255) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGAt > 1:15816/1‑47 (MQ=255) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAttt > 1:380661/1‑71 (MQ=255) cAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGcaat < 1:2393310/69‑4 (MQ=0) aaaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATa < 1:3633402/47‑1 (MQ=255) aaaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCg < 1:3844684/51‑1 (MQ=255) aaaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCa < 1:3614853/61‑1 (MQ=255) aaaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCa < 1:1851692/61‑1 (MQ=255) aaaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCa < 1:962781/61‑1 (MQ=255) aaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGc > 1:227844/1‑71 (MQ=255) aaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGc > 1:2928902/1‑71 (MQ=255) aaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGc > 1:983841/1‑71 (MQ=255) tCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGt > 1:725990/1‑71 (MQ=255) tCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGt < 1:3388900/71‑1 (MQ=255) tCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGt > 1:2420039/1‑71 (MQ=255) | TCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGT > minE/2155522‑2155661 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |