| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,155,607 | T→A | 7.6% | intergenic (+118/+68) | dacB → / ← obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,155,607 | 0 | T | A | 7.6% | 181.4 / 6.5 | 66 | intergenic (+118/+68) | dacB/obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Reads supporting (aligned to +/- strand): ref base T (40/21); new base A (3/2); total (43/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01 | |||||||||||
GTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATT > minE/2155538‑2155676 | gTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACata < 1:1502857/71‑1 (MQ=39) tGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACatat > 1:3163074/1‑71 (MQ=39) cgcTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTg > 1:592977/1‑71 (MQ=39) tttATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtc < 1:112347/70‑1 (MQ=255) tATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTc‑tct > 1:160016/1‑71 (MQ=255) ccGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTa < 1:757168/71‑1 (MQ=255) ggATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAg < 1:2820287/70‑1 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAgg > 1:378758/1‑71 (MQ=11) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCt > 1:2547970/1‑71 (MQ=255) gcggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCt > 1:1473641/1‑70 (MQ=255) ggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGa > 1:1435700/1‑70 (MQ=255) ggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGAt < 1:2525497/71‑1 (MQ=255) ggcgTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGa > 1:3172492/1‑71 (MQ=14) cgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTg < 1:204757/48‑1 (MQ=255) cgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATaa < 1:1881545/71‑1 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGc > 1:3349055/1‑60 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCg > 1:1649692/1‑70 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCg > 1:2966096/1‑70 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCg > 1:1818822/1‑70 (MQ=255) aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCCACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGtc > 1:900512/1‑70 (MQ=255) aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCg > 1:505943/1‑69 (MQ=255) ccTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAgcg > 1:1178601/1‑71 (MQ=255) cTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCaa > 1:634349/1‑42 (MQ=40) cTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCgca < 1:1723047/68‑3 (MQ=11) tATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:3104888/1‑71 (MQ=255) aTCCGGCCTACAAAATCGTGCAAATTCAACATATTGCTATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:828670/1‑70 (MQ=255) cGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAgg < 1:669121/71‑1 (MQ=255) gCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCt > 1:1363920/1‑71 (MQ=255) ccTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGc > 1:627270/1‑69 (MQ=255) ccTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCt > 1:1067304/1‑70 (MQ=255) ccTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCt > 1:1387815/1‑70 (MQ=255) cTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg < 1:2097864/40‑1 (MQ=255) cTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGcaa > 1:1006971/1‑69 (MQ=2) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGt > 1:2639210/1‑38 (MQ=255) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGAt > 1:15816/1‑47 (MQ=255) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAttt > 1:380661/1‑71 (MQ=255) cAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGcaat < 1:2393310/69‑4 (MQ=0) aaaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATa < 1:3633402/47‑1 (MQ=255) aaaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCg < 1:3844684/51‑1 (MQ=255) aaaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCa < 1:962781/61‑1 (MQ=255) aaaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCa < 1:3614853/61‑1 (MQ=255) aaaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCa < 1:1851692/61‑1 (MQ=255) aaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGc > 1:983841/1‑71 (MQ=255) aaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGc > 1:227844/1‑71 (MQ=255) aaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGc > 1:2928902/1‑71 (MQ=255) tCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGt < 1:3388900/71‑1 (MQ=255) tCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGt > 1:2420039/1‑71 (MQ=255) tCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGt > 1:725990/1‑71 (MQ=255) cGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGtt > 1:1361377/1‑71 (MQ=255) cGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGtt > 1:2834110/1‑71 (MQ=255) gCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTat > 1:36949/1‑71 (MQ=255) aaaTTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTa < 1:1476321/43‑1 (MQ=255) aaaTTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGttt < 1:265077/67‑1 (MQ=255) aaTTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAtt > 1:2471532/1‑58 (MQ=255) aaTTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGttt < 1:2116251/66‑1 (MQ=255) aaTTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTatcat > 1:354385/1‑71 (MQ=255) aTTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCg < 1:3824181/39‑1 (MQ=255) aTTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTatcat > 1:2639318/1‑70 (MQ=255) aTTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTatcat > 1:643573/1‑70 (MQ=255) aTTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTatcat > 1:641304/1‑70 (MQ=255) ttCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGTCGTTTAtcatca < 1:3049201/71‑1 (MQ=255) tCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGc > 1:2589245/1‑36 (MQ=255) tCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTa > 1:1665264/1‑39 (MQ=255) aaCATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGt < 1:3788766/70‑1 (MQ=255) aCATATTGCAATTC‑TCTAGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCACTg > 1:2637782/1‑70 (MQ=255) cATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTatcat < 1:1192717/64‑1 (MQ=255) cATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGAt > 1:1179388/1‑71 (MQ=255) cATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGAt > 1:3320652/1‑71 (MQ=255) atatTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGAtt < 1:2267692/71‑1 (MQ=255) atatTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGAtt < 1:522826/71‑1 (MQ=255) | GTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATT > minE/2155538‑2155676 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |