| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,220,170 | C→T | 6.3% | intergenic (+430/‑548) | yhdH → / → accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,220,170 | 0 | C | T | 6.3% | 192.5 / 2.8 | 63 | intergenic (+430/‑548) | yhdH/accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Reads supporting (aligned to +/- strand): ref base C (35/24); new base T (2/2); total (37/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
AATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATC > minE/2220100‑2220238 | aaTTTTGCGTGCTTTAGCATTCACATCTATCCAGCCGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGc > 1:1997485/1‑71 (MQ=255) aTTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGc < 1:591981/70‑1 (MQ=255) ttttGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCt < 1:2979991/70‑1 (MQ=255) gCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGt < 1:3239952/70‑1 (MQ=255) gTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAAt < 1:697935/71‑1 (MQ=255) gCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGc < 1:2441249/64‑1 (MQ=255) gCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATgt > 1:3944376/1‑71 (MQ=255) gCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATgt > 1:2475417/1‑71 (MQ=255) cTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATgtc > 1:2057980/1‑71 (MQ=255) tttAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATgtcg > 1:2146503/1‑71 (MQ=255) tAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGc > 1:87410/1‑57 (MQ=255) gCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCt < 1:2291234/70‑1 (MQ=255) gCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTa < 1:405044/71‑1 (MQ=255) cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTa > 1:1910624/1‑70 (MQ=255) aTTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATc > 1:621619/1‑71 (MQ=255) ttCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCt < 1:1686243/71‑1 (MQ=255) cacaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCtt < 1:1968711/70‑1 (MQ=255) cacaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTg > 1:331521/1‑71 (MQ=255) acaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTg < 1:2457842/70‑1 (MQ=255) acaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGt > 1:2760250/1‑71 (MQ=255) cTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTg < 1:383831/66‑1 (MQ=255) cTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTcgc > 1:1004568/1‑70 (MQ=255) aTCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATATTGTcgcg > 1:2683456/1‑69 (MQ=255) tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTg > 1:3491597/1‑63 (MQ=255) cccAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGAtt < 1:2322840/70‑2 (MQ=255) cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCt < 1:1963329/71‑1 (MQ=255) aGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTg > 1:1632614/1‑71 (MQ=255) aCGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGc < 1:1735119/71‑1 (MQ=255) cGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCa > 1:3223493/1‑71 (MQ=255) gATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGc > 1:3273377/1‑69 (MQ=255) tGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTcgc < 1:1205496/58‑1 (MQ=255) tGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCATGTCGCGATCCTGGCATcc > 1:725309/1‑71 (MQ=255) tGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGc > 1:2068044/1‑67 (MQ=255) gCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTg > 1:1491964/1‑53 (MQ=255) cAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc > 1:2237226/1‑70 (MQ=255) aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATgtcg > 1:639480/1‑38 (MQ=255) aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGa > 1:3417036/1‑53 (MQ=255) aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGAt > 1:904695/1‑54 (MQ=255) aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACat > 1:1959895/1‑70 (MQ=255) aaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTg > 1:1776963/1‑46 (MQ=255) aaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACatta < 1:537918/71‑1 (MQ=255) aaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACa > 1:3355265/1‑68 (MQ=255) aaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTcgc > 1:3066925/1‑49 (MQ=255) aTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTcgc > 1:3885006/1‑48 (MQ=255) aTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt > 1:1730527/1‑71 (MQ=255) aTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt > 1:362339/1‑71 (MQ=255) gggTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTtg < 1:3593622/70‑1 (MQ=255) ggTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTAttt < 1:3064823/68‑1 (MQ=255) tAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTgg > 1:899234/1‑70 (MQ=255) aaTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTc > 1:3277027/1‑71 (MQ=255) aaTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTc > 1:1333357/1‑71 (MQ=255) tCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCt > 1:1493443/1‑70 (MQ=255) ccccAGCAACCGCTGCGTAATGTCGTCTATCTTGTcgcg < 1:2165458/39‑1 (MQ=255) cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTa > 1:3750518/1‑53 (MQ=255) cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg < 1:2076917/71‑1 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATcc < 1:3631409/38‑1 (MQ=255) gCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCcgt > 1:746696/1‑66 (MQ=255) gCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAg < 1:2025432/70‑1 (MQ=255) aaacGCTGCGTAATGGCGTCTATCTTGTCGCGATCCTGGCTTGCCTACATTATTTGTGGTCTTTGAAGAtt < 1:932014/68‑1 (MQ=255) aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATc > 1:1554008/1‑35 (MQ=255) aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAtt < 1:3621841/71‑1 (MQ=255) aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAtt < 1:1592181/71‑1 (MQ=255) aCCGCTGCGTAATGTCGTCTATCTTGTCGCGATcg < 1:114431/35‑2 (MQ=255) cGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtc < 1:560546/71‑1 (MQ=255) gCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACa > 1:3457964/1‑45 (MQ=255) gCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACa > 1:181925/1‑45 (MQ=255) | AATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATC > minE/2220100‑2220238 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |