Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 311,511 T→A 14.8% V454V (GTA→GTT) ‡ acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE311,5110TA14.8% 208.5 / 26.7 81V454V (GTA→GTT) ‡acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (37/32);  new base A (8/4);  total (45/36)
Fisher's exact test for biased strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

TGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGG  >  minE/311442‑311581
                                                                     |                                                                      
tGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa                                                                       >  1:3122340/1‑71 (MQ=255)
     aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg                                                                     <  1:2689047/68‑1 (MQ=255)
     aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGaa                                                                   >  1:574802/1‑70 (MQ=255)
     aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAAt                                                                  <  1:3545284/71‑1 (MQ=255)
        cAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAcc                                                               >  1:2666186/1‑71 (MQ=255)
           tGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgcc                                                            >  1:48657/1‑71 (MQ=255)
              tAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa                                                          >  1:2841052/1‑70 (MQ=255)
              tAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa                                                          >  1:3192846/1‑70 (MQ=255)
              tAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa                                                          >  1:702752/1‑70 (MQ=255)
                 tAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa                                                                       >  1:410310/1‑54 (MQ=255)
                    agaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATa                                                                 >  1:348051/1‑57 (MQ=255)
                    agaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAcc                                                   <  1:2784490/71‑1 (MQ=255)
                     gaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa                                                  >  1:960071/1‑71 (MQ=255)
                       aCTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCAt                                                 <  1:880954/70‑1 (MQ=9)
                       aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCAt                                                 <  1:2531350/70‑1 (MQ=255)
                       aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATc                                                <  1:3754158/71‑1 (MQ=255)
                       aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATc                                                <  1:2104716/71‑1 (MQ=255)
                       aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCAGCGGTACGAATACCGCCGACAGTACCATc                                                <  1:1121581/71‑1 (MQ=255)
                        atGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcg                                               >  1:1725338/2‑71 (MQ=255)
                         tGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcg                                               >  1:2672090/1‑70 (MQ=255)
                         tGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGAACCAt                                                 <  1:1535646/68‑1 (MQ=255)
                           aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa                                            >  1:1412848/1‑71 (MQ=255)
                             gatagataGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATa                                          <  1:1264754/71‑1 (MQ=255)
                             gatagataGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATa                                          >  1:2727115/1‑71 (MQ=255)
                               tagatagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgcc                                                            <  1:443508/51‑1 (MQ=255)
                               tagatagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATAcc                                        <  1:812398/71‑1 (MQ=255)
                                     gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCag                                  <  1:3266831/71‑1 (MQ=255)
                                     gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCag                                  <  1:177690/71‑1 (MQ=255)
                                     gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa                                   <  1:3471492/70‑1 (MQ=255)
                                     gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa                                   <  1:2442503/70‑1 (MQ=255)
                                     gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa                                   <  1:3768237/70‑1 (MQ=255)
                                      ccccAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCagt                                 >  1:3500801/3‑70 (MQ=255)
                                      cACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGaa                                                                   <  1:105259/37‑1 (MQ=255)
                                      cACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCaga                                 <  1:136443/71‑1 (MQ=255)
                                       aCCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAg                                                       <  1:266734/48‑1 (MQ=255)
                                       aCCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAg                                                       <  1:664219/48‑1 (MQ=255)
                                       aCCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAg                                                       <  1:2882105/48‑1 (MQ=255)
                                       aCCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCagag                                <  1:3841463/71‑1 (MQ=255)
                                       aCCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCagag                                <  1:2957357/71‑1 (MQ=255)
                                         cAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATAc                                         >  1:1743140/1‑60 (MQ=18)
                                         cAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgc                                                             >  1:3775808/1‑40 (MQ=255)
                                         cAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCaga                                 >  1:2827285/1‑68 (MQ=255)
                                         cAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAACATCGCGATACCGACCAGAgc                               >  1:3517937/1‑70 (MQ=255)
                                          aGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCAc                                                    <  1:3759833/48‑1 (MQ=12)
                                          aGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTgcg                              >  1:3705393/1‑70 (MQ=255)
                                          aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAgcgc                             >  1:556739/1‑71 (MQ=255)
                                           gTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGcc                            >  1:2353725/1‑71 (MQ=255)
                                            tAGAGCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGt                                                      >  1:2681797/1‑44 (MQ=255)
                                            tAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGAt                                           <  1:1074038/55‑1 (MQ=255)
                                              gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt                          >  1:3370656/1‑70 (MQ=255)
                                              gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt                          >  1:3637324/1‑70 (MQ=255)
                                               aaCCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGa                        <  1:3401176/71‑1 (MQ=255)
                                                aCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATa                                          <  1:1926786/52‑1 (MQ=255)
                                                aCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGaa                       >  1:2304363/1‑71 (MQ=255)
                                                  cGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAAtt                     <  1:3165198/71‑2 (MQ=255)
                                                  cGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa                                   >  1:1067930/1‑57 (MQ=255)
                                                   gCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAAttt                    >  1:3818268/1‑69 (MQ=255)
                                                   gCCAAAGAAGGCCATCGGTACTAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt                    <  1:3610260/71‑1 (MQ=255)
                                                   gCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt                    <  1:2487857/71‑1 (MQ=255)
                                                     cgaagaatGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATa                                          >  1:250651/3‑47 (MQ=16)
                                                     cgaagaatGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGc                  >  1:2403519/3‑71 (MQ=255)
                                                     cAAAGAAGGCCATCGGTACGAATACCGCCGACACTACCATCGCGATACCGACCAGAGCGCCCTGa                        >  1:2256322/1‑65 (MQ=255)
                                                      aaaGAAGGCCATCGGTACGAATACCGCCGACAGTACCAt                                                 >  1:1177553/1‑39 (MQ=255)
                                                      aaaGAAGGCCATCGGTACGAATACCGCCGACAGTACCAt                                                 >  1:2674628/1‑39 (MQ=255)
                                                      aaaGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGcc                 >  1:403912/1‑71 (MQ=255)
                                                       aagaatGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATAccca                                      >  1:228887/1‑47 (MQ=16)
                                                       aagaagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGccc                >  1:1450726/1‑71 (MQ=255)
                                                        agaagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTg                         >  1:2517569/1‑61 (MQ=255)
                                                          aagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCAt             >  1:3391678/1‑71 (MQ=255)
                                                            ggCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGaa                       >  1:2542684/1‑59 (MQ=255)
                                                            ggCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCg           <  1:2045518/71‑1 (MQ=255)
                                                              ccATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGat         >  1:976105/1‑70 (MQ=255)
                                                               cATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGa                        <  1:1269278/55‑1 (MQ=255)
                                                                 tCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt                    <  1:1606618/57‑1 (MQ=255)
                                                                 tCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTa      >  1:361136/1‑71 (MQ=255)
                                                                  cGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc     >  1:901156/1‑71 (MQ=255)
                                                                  cGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc     >  1:905829/1‑71 (MQ=255)
                                                                   ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc     <  1:2640374/70‑1 (MQ=255)
                                                                   ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc     <  1:2000163/70‑1 (MQ=255)
                                                                   ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACg    >  1:2299603/1‑71 (MQ=255)
                                                                   ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACg    >  1:3086170/1‑71 (MQ=255)
                                                                     tACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACggg  <  1:1733761/71‑1 (MQ=255)
                                                                     |                                                                      
TGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGG  >  minE/311442‑311581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: