| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,220,199 | C→T | 15.8% | intergenic (+459/‑519) | yhdH → / → accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,220,199 | 0 | C | T | 15.8% | 150.4 / 17.9 | 63 | intergenic (+459/‑519) | yhdH/accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Reads supporting (aligned to +/- strand): ref base C (29/24); new base T (5/5); total (34/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.69e-01 | |||||||||||
CAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTG > minE/2220131‑2220268 | cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCt < 1:1963329/71‑1 (MQ=255) aGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTg > 1:1632614/1‑71 (MQ=255) aCGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGc < 1:1735119/71‑1 (MQ=255) cGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCa > 1:3223493/1‑71 (MQ=255) gATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGc > 1:3273377/1‑69 (MQ=255) tGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCATGTCGCGATCCTGGCATcc > 1:725309/1‑71 (MQ=255) tGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGc > 1:2068044/1‑67 (MQ=255) cAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc > 1:2237226/1‑70 (MQ=255) aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACat > 1:1959895/1‑70 (MQ=255) aaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACatta < 1:537918/71‑1 (MQ=255) aaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACa > 1:3355265/1‑68 (MQ=255) aTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt > 1:362339/1‑71 (MQ=255) aTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt > 1:1730527/1‑71 (MQ=255) gggTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTtg < 1:3593622/70‑1 (MQ=255) ggTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTAttt < 1:3064823/68‑1 (MQ=255) tAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTgg > 1:899234/1‑70 (MQ=255) aaTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTc > 1:3277027/1‑71 (MQ=255) aaTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTc > 1:1333357/1‑71 (MQ=255) tCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCt > 1:1493443/1‑70 (MQ=255) cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTa > 1:3750518/1‑53 (MQ=255) cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg < 1:2076917/71‑1 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATcc < 1:3631409/38‑1 (MQ=255) gCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCcgt > 1:746696/1‑66 (MQ=255) gCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAg < 1:2025432/70‑1 (MQ=255) aaacGCTGCGTAATGGCGTCTATCTTGTCGCGATCCTGGCTTGCCTACATTATTTGTGGTCTTTGAAGAtt < 1:932014/68‑1 (MQ=255) aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATc > 1:1554008/1‑35 (MQ=255) aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAtt < 1:3621841/71‑1 (MQ=255) aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAtt < 1:1592181/71‑1 (MQ=255) aCCGCTGCGTAATGTCGTCTATCTTGTCGCGATcg < 1:114431/35‑2 (MQ=255) cGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtc < 1:560546/71‑1 (MQ=255) gCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACa > 1:181925/1‑45 (MQ=255) gCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACa > 1:3457964/1‑45 (MQ=255) gcatAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGGCCGTGAAGATTATTTCa < 1:2331853/67‑1 (MQ=255) gCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCAt > 1:3208725/1‑71 (MQ=255) cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCa < 1:1021865/69‑1 (MQ=255) cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCa < 1:3613836/69‑1 (MQ=255) cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCAtt < 1:1665413/71‑1 (MQ=255) tAATGTCGTCTATCTTGTCGCGATCCTGGCATccc < 1:2851323/35‑1 (MQ=255) aTGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAg < 1:3578032/71‑1 (MQ=255) aTGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAg < 1:3357673/71‑1 (MQ=255) tGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattat < 1:2685490/40‑1 (MQ=255) cgtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGcccc > 1:43452/1‑71 (MQ=255) gtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGcccat > 1:2706800/1‑69 (MQ=255) gtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCt > 1:1692820/1‑71 (MQ=255) cTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCa > 1:263531/1‑71 (MQ=255) tATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCAt > 1:1218499/1‑71 (MQ=255) tATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGAAGCCGCTCa > 1:1786018/1‑70 (MQ=255) tATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCAt > 1:2893/1‑71 (MQ=255) aTCTTGTCGCGATTCTGGCCCCAATACATTATCTGTgg < 1:113801/38‑1 (MQ=255) aTCTTGTCGCGATTCTGGCCCCAATACATTATCTGTgg < 1:3500207/38‑1 (MQ=255) aTCTTGTCGCGATTCTGGCCCCAATACATTATCTGTgg < 1:1514676/38‑1 (MQ=255) aTCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATc > 1:3737366/1‑71 (MQ=255) tCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCt > 1:1963595/1‑71 (MQ=255) cTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGt > 1:719520/1‑37 (MQ=255) gTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTc < 1:2189772/69‑1 (MQ=255) tCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgt < 1:1790647/37‑1 (MQ=255) tCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTg < 1:3474209/53‑1 (MQ=255) tCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCa < 1:2276963/71‑1 (MQ=255) cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGa > 1:1734788/1‑36 (MQ=255) cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGa > 1:2477830/1‑36 (MQ=255) cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGa > 1:2482351/1‑36 (MQ=255) cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAgg < 1:2946723/70‑1 (MQ=255) aTCCTGGCATCCCTACATTATTTGTGGTTTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggct > 1:2966677/1‑71 (MQ=255) aTCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggct > 1:3882101/1‑71 (MQ=255) tCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTa < 1:3620748/39‑1 (MQ=255) tCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggctg < 1:2441963/71‑1 (MQ=255) | CAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTG > minE/2220131‑2220268 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |