Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,220,206 T→C 14.7% intergenic (+466/‑512) yhdH → / → accB predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,220,2060TC14.7% 143.5 / 16.9 61intergenic (+466/‑512)yhdH/accBpredicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit
Reads supporting (aligned to +/- strand):  ref base T (27/25);  new base C (4/5);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

TGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTT  >  minE/2220138‑2220274
                                                                    |                                                                    
tGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCATGTCGCGATCCTGGCATcc                                                                    >  1:725309/1‑71 (MQ=255)
  cAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                                                                   >  1:2237226/1‑70 (MQ=255)
       aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACat                                                              >  1:1959895/1‑70 (MQ=255)
        aaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACatta                                                            <  1:537918/71‑1 (MQ=255)
        aaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACa                                                               >  1:3355265/1‑68 (MQ=255)
          aTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt                                                          >  1:362339/1‑71 (MQ=255)
          aTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt                                                          >  1:1730527/1‑71 (MQ=255)
             gggTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTtg                                                        <  1:3593622/70‑1 (MQ=255)
              ggTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTAttt                                                         <  1:3064823/68‑1 (MQ=255)
                tAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTgg                                                     >  1:899234/1‑70 (MQ=255)
                 aaTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTc                                                   >  1:3277027/1‑71 (MQ=255)
                 aaTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTc                                                   >  1:1333357/1‑71 (MQ=255)
                   tCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCt                                                  >  1:1493443/1‑70 (MQ=255)
                     cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTa                                                                 >  1:3750518/1‑53 (MQ=255)
                     cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg                                               <  1:2076917/71‑1 (MQ=255)
                         gCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCcgt                                                >  1:746696/1‑66 (MQ=255)
                         gCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAg                                            <  1:2025432/70‑1 (MQ=255)
                           aaacGCTGCGTAATGGCGTCTATCTTGTCGCGATCCTGGCTTGCCTACATTATTTGTGGTCTTTGAAGAtt                                         <  1:932014/68‑1 (MQ=255)
                           aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAtt                                         <  1:3621841/71‑1 (MQ=255)
                           aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAtt                                         <  1:1592181/71‑1 (MQ=255)
                              cGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtc                                      <  1:560546/71‑1 (MQ=255)
                               gCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACa                                                               >  1:181925/1‑45 (MQ=255)
                               gCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACa                                                               >  1:3457964/1‑45 (MQ=255)
                                  gcatAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGGCCGTGAAGATTATTTCa                                   <  1:2331853/67‑1 (MQ=255)
                                  gCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCAt                                  >  1:3208725/1‑71 (MQ=255)
                                   cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCa                                   <  1:3613836/69‑1 (MQ=255)
                                   cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCa                                   <  1:1021865/69‑1 (MQ=255)
                                   cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCAtt                                 <  1:1665413/71‑1 (MQ=255)
                                     tAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                                                                   <  1:2851323/35‑1 (MQ=255)
                                       aTGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAg                             <  1:3578032/71‑1 (MQ=255)
                                       aTGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAg                             <  1:3357673/71‑1 (MQ=255)
                                        tGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattat                                                           <  1:2685490/40‑1 (MQ=255)
                                           cgtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGcccc                         >  1:43452/1‑71 (MQ=255)
                                            gtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGcccat                        >  1:2706800/1‑69 (MQ=255)
                                            gtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCt                        >  1:1692820/1‑71 (MQ=255)
                                              cTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCa                      >  1:263531/1‑71 (MQ=255)
                                               tATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCAt                     >  1:1218499/1‑71 (MQ=255)
                                               tATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGAAGCCGCTCa                      >  1:1786018/1‑70 (MQ=255)
                                               tATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCAt                     >  1:2893/1‑71 (MQ=255)
                                                aTCTTGTCGCGATTCTGGCCCCAATACATTATCTGTgg                                                     <  1:1514676/38‑1 (MQ=255)
                                                aTCTTGTCGCGATTCTGGCCCCAATACATTATCTGTgg                                                     <  1:3500207/38‑1 (MQ=255)
                                                aTCTTGTCGCGATTCTGGCCCCAATACATTATCTGTgg                                                     <  1:113801/38‑1 (MQ=255)
                                                aTCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATc                    >  1:3737366/1‑71 (MQ=255)
                                                 tCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCt                   >  1:1963595/1‑71 (MQ=255)
                                                  cTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGt                                                    >  1:719520/1‑37 (MQ=255)
                                                     gTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTc                 <  1:2189772/69‑1 (MQ=255)
                                                      tCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgt                                                <  1:1790647/37‑1 (MQ=255)
                                                      tCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTg                                <  1:3474209/53‑1 (MQ=255)
                                                      tCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCa              <  1:2276963/71‑1 (MQ=255)
                                                         cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGa                                              >  1:2482351/1‑36 (MQ=255)
                                                         cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGa                                              >  1:1734788/1‑36 (MQ=255)
                                                         cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGa                                              >  1:2477830/1‑36 (MQ=255)
                                                         cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAgg            <  1:2946723/70‑1 (MQ=255)
                                                           aTCCTGGCATCCCTACATTATTTGTGGTTTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggct         >  1:2966677/1‑71 (MQ=255)
                                                           aTCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggct         >  1:3882101/1‑71 (MQ=255)
                                                            tCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTa                                        <  1:3620748/39‑1 (MQ=255)
                                                            tCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggctg        <  1:2441963/71‑1 (MQ=255)
                                                                 gCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttt   <  1:2678425/71‑1 (MQ=255)
                                                                 gCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttt   <  1:2145023/71‑1 (MQ=255)
                                                                  cATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAg             <  1:1018372/60‑1 (MQ=255)
                                                                   aTCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGcctttt  >  1:3007249/1‑70 (MQ=255)
                                                                    |                                                                    
TGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTT  >  minE/2220138‑2220274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: