| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,220,238 | C→T | 9.4% | intergenic (+498/‑480) | yhdH → / → accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,220,238 | 0 | C | T | 9.4% | 147.6 / 4.8 | 53 | intergenic (+498/‑480) | yhdH/accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Reads supporting (aligned to +/- strand): ref base C (18/30); new base T (3/2); total (21/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
CGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCC > minE/2220168‑2220305 | cGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtc < 1:560546/71‑1 (MQ=255) gcatAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGGCCGTGAAGATTATTTCa < 1:2331853/67‑1 (MQ=255) gCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCAt > 1:3208725/1‑71 (MQ=255) cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCa < 1:1021865/69‑1 (MQ=255) cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCa < 1:3613836/69‑1 (MQ=255) cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCAtt < 1:1665413/71‑1 (MQ=255) aTGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAg < 1:3357673/71‑1 (MQ=255) aTGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAg < 1:3578032/71‑1 (MQ=255) cgtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGcccc > 1:43452/1‑71 (MQ=255) gtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGcccat > 1:2706800/1‑69 (MQ=255) gtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCt > 1:1692820/1‑71 (MQ=255) cTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCa > 1:263531/1‑71 (MQ=255) tATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCAt > 1:1218499/1‑71 (MQ=255) tATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGAAGCCGCTCa > 1:1786018/1‑70 (MQ=255) tATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCAt > 1:2893/1‑71 (MQ=255) aTCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATc > 1:3737366/1‑71 (MQ=255) tCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCt > 1:1963595/1‑71 (MQ=255) gTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTc < 1:2189772/69‑1 (MQ=255) tCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTg < 1:3474209/53‑1 (MQ=255) tCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCa < 1:2276963/71‑1 (MQ=255) cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAgg < 1:2946723/70‑1 (MQ=255) aTCCTGGCATCCCTACATTATTTGTGGTTTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggct > 1:2966677/1‑71 (MQ=255) aTCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggct > 1:3882101/1‑71 (MQ=255) tCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggctg < 1:2441963/71‑1 (MQ=255) gCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttt < 1:2145023/71‑1 (MQ=255) gCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttt < 1:2678425/71‑1 (MQ=255) cATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAg < 1:1018372/60‑1 (MQ=255) aTCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGcctttt > 1:3007249/1‑70 (MQ=255) ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGc > 1:2367900/1‑38 (MQ=255) ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagc < 1:874545/71‑1 (MQ=255) ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCATCATCTTCGCAGGGCTGgcttttcagc < 1:3012277/71‑1 (MQ=255) cTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagct > 1:85787/1‑71 (MQ=255) cTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagct > 1:3951021/1‑71 (MQ=255) aCATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagcttt > 1:230707/1‑71 (MQ=255) cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGc > 1:2659388/1‑50 (MQ=255) cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagcttt < 1:1748476/70‑1 (MQ=255) ttattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctttt < 1:1858827/69‑1 (MQ=255) tattTGTGGTCCGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctttt < 1:3894412/68‑1 (MQ=255) tttGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCtt < 1:2077782/42‑1 (MQ=255) gattgGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCtt < 1:1793023/68‑1 (MQ=255) tgtgGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCtt < 1:365151/70‑1 (MQ=255) tgtgGGCTGTGAAGATTATCTCATTGCAGCCCCTCATCtt < 1:3173215/40‑1 (MQ=255) tgGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGCTGGGCTGGCTGTACTGCTTTTAGCCTTACg > 1:1629281/1‑71 (MQ=255) tgtgAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagcttttc < 1:2495725/58‑1 (MQ=255) tgtgAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTtat < 1:341319/70‑1 (MQ=255) tgAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaag < 1:2770125/71‑1 (MQ=255) aaGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttt < 1:2309994/44‑1 (MQ=255) aaGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaagaa < 1:3737436/71‑1 (MQ=255) aaGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACCTTATaaga < 1:3274971/70‑1 (MQ=255) aGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagcttttca < 1:3621684/54‑1 (MQ=255) aTTATCTCATTGCAGCCCCTCATCTTCGCAGggct > 1:1956931/1‑35 (MQ=255) ttATTTCACCGCAGCCGCTCATCTACGCTGGGCTGGCTGTACTGCTTTTAGCCTTACGGTATAAGAAGtt < 1:306202/70‑1 (MQ=255) tATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTcc > 1:1724198/1‑71 (MQ=255) aTCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTcc < 1:555951/70‑1 (MQ=255) | CGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCC > minE/2220168‑2220305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |