| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,236,063 | G→A | 7.3% | V403V (GTG→GTA) | purH → | fused IMP cyclohydrolase and phosphoribosylaminoimidazolecarboxamide formyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,236,063 | 0 | G | A | 7.3% | 246.3 / 7.3 | 82 | V403V (GTG→GTA) | purH | fused IMP cyclohydrolase and phosphoribosylaminoimidazolecarboxamide formyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (43/33); new base A (4/2); total (47/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
ACGCGTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCGAAGTTT > minE/2235994‑2236132 | aCGCGTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCgtgg > 1:3357476/1‑71 (MQ=255) cgcgTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCgtggt > 1:50957/1‑71 (MQ=255) cgcgTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCgtggt < 1:741185/71‑1 (MQ=255) gcgTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCgtggtg < 1:3752138/71‑1 (MQ=255) gTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGAc > 1:2821905/1‑71 (MQ=255) gTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGAc > 1:87156/1‑71 (MQ=255) gTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGAc > 1:550322/1‑71 (MQ=255) gAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGAcc > 1:2887376/1‑70 (MQ=255) gAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGAcc > 1:216843/1‑70 (MQ=255) gAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGAcc > 1:212180/1‑70 (MQ=255) aaCGGCGGTCTGCTGGTTCAGGATCGTGACCTTGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCaa < 1:3385548/71‑1 (MQ=255) aaCGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCaa < 1:2693917/71‑1 (MQ=255) aaCGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCa < 1:2040977/70‑1 (MQ=255) aaCGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCa < 1:2967828/70‑1 (MQ=255) cggcggTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAAc > 1:1105850/1‑71 (MQ=255) cggcggTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTAGTGACCaaa > 1:522959/1‑70 (MQ=255) gcggTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGt < 1:3872289/71‑1 (MQ=255) cggTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCgtgg < 1:1269835/59‑1 (MQ=255) ggTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCa < 1:2504313/64‑1 (MQ=255) ggTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCa < 1:1228681/64‑1 (MQ=255) gTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAg > 1:1945837/1‑71 (MQ=255) tCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGc > 1:1193593/1‑71 (MQ=255) ctgctgGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCgtggt > 1:1858228/1‑56 (MQ=255) tgctgGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGcc > 1:3549680/1‑70 (MQ=255) tgctgGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGcc > 1:3127356/1‑70 (MQ=255) ctgGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGa < 1:3789495/55‑1 (MQ=255) ctgGTTCAGGATCGTGACCTGGGGATGGTCGGTGCAGAAGAACTGCGCGTGGTGa < 1:3448039/55‑1 (MQ=255) gTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCg < 1:1937503/70‑1 (MQ=255) gTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCg > 1:897621/1‑70 (MQ=255) cAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCgtggt > 1:1528728/1‑47 (MQ=255) cAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGaa > 1:1789760/1‑69 (MQ=255) cAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGaa > 1:3092543/1‑69 (MQ=255) cAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGaa > 1:1127567/1‑69 (MQ=255) aGGATCGTGACCTGTGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAg > 1:1949934/1‑71 (MQ=255) aGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAAGGTCAGCCGAGCGAAc < 1:2723678/69‑1 (MQ=255) aGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAg < 1:315723/71‑1 (MQ=255) ggATCGTGACCTGGGTATGGTCGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAgg < 1:1232419/71‑1 (MQ=255) ggATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAg < 1:2146291/58‑1 (MQ=255) gATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAAc > 1:1522764/1‑52 (MQ=255) gATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAgg > 1:2621728/1‑70 (MQ=255) aTCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCaaa > 1:3344144/1‑50 (MQ=255) aTCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCaaa > 1:3691751/1‑50 (MQ=255) aTCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCaaa > 1:2794586/1‑50 (MQ=255) tCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAAc > 1:991487/1‑71 (MQ=255) tGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACt < 1:379868/69‑1 (MQ=255) tGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGc > 1:3408754/1‑71 (MQ=255) gACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGc > 1:145741/1‑70 (MQ=255) aCCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGc > 1:1606149/1‑69 (MQ=255) aCCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCg < 1:218925/70‑1 (MQ=255) aCCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGt > 1:207896/1‑71 (MQ=255) aCCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGt < 1:1881565/71‑1 (MQ=255) cTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCa > 1:3455739/1‑40 (MQ=255) cTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTc > 1:2001788/1‑46 (MQ=255) gggATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATgc > 1:925057/1‑71 (MQ=255) ggATGGTCGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATgcg > 1:1587681/1‑71 (MQ=255) gATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATgcgc > 1:1466455/1‑71 (MQ=255) gATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATgcgc > 1:3738092/1‑71 (MQ=255) gTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGtt < 1:3675226/71‑1 (MQ=255) gTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGt < 1:3248666/70‑1 (MQ=255) tCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGaa < 1:1991946/47‑1 (MQ=255) tCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTg > 1:2431803/1‑68 (MQ=255) tCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTc < 1:1861229/71‑1 (MQ=255) cGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCg > 1:1653622/1‑57 (MQ=255) cGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTct > 1:1476088/1‑71 (MQ=255) ggTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTct < 1:1942758/70‑1 (MQ=255) gCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGaa > 1:2816024/1‑42 (MQ=255) gCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTctgctg > 1:1508152/1‑71 (MQ=255) gaagaaCTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGa < 1:2069400/70‑1 (MQ=255) aagaaCTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAg > 1:2969952/1‑71 (MQ=255) aagaaCTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAg > 1:114068/1‑71 (MQ=255) aagaaCTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAg < 1:1734000/71‑1 (MQ=255) gaaCTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTTGAAGGt < 1:2445000/71‑1 (MQ=255) gaaCTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGt > 1:476551/1‑71 (MQ=255) aCTGCGCGTGGTGACCAAACGTCAGCCTAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTgg < 1:3715445/71‑1 (MQ=255) aCTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTgg > 1:708272/1‑71 (MQ=255) aCTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTgg > 1:635947/1‑71 (MQ=255) aCTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTgg < 1:1662669/71‑1 (MQ=255) aCTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTgg > 1:87885/1‑71 (MQ=255) aCTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTgg > 1:2461984/1‑71 (MQ=255) cTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGc < 1:689216/71‑1 (MQ=255) cTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGc < 1:2347472/71‑1 (MQ=255) tGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGc > 1:1660803/1‑70 (MQ=255) cgcgTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCg < 1:3828809/69‑1 (MQ=255) cgcgTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCg > 1:711674/1‑69 (MQ=255) cgcgTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCGa < 1:830089/70‑1 (MQ=255) cgcgTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCg < 1:2542261/69‑1 (MQ=255) cgTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCGAAGt < 1:2412103/71‑1 (MQ=255) cgTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCGAAGt < 1:1413554/71‑1 (MQ=255) cgTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCGAAGt < 1:3531321/71‑1 (MQ=255) gtggtgACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCGAAGtt < 1:90884/71‑1 (MQ=255) tggtgACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCGAAGttt < 1:2384842/71‑1 (MQ=255) tggtgACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCGAAGttt < 1:1799848/71‑1 (MQ=255) | ACGCGTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGGATGGTCGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAGGTGGCGAAGTTT > minE/2235994‑2236132 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |