| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,245,573 | T→C | 17.0% | intergenic (‑89/‑6) | nudC ← / → rsd | NADH pyrophosphatase/stationary phase protein, binds sigma 70 RNA polymerase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,245,573 | 0 | T | C | 17.0% | 114.3 / 17.7 | 53 | intergenic (‑89/‑6) | nudC/rsd | NADH pyrophosphatase/stationary phase protein, binds sigma 70 RNA polymerase subunit |
| Reads supporting (aligned to +/- strand): ref base T (22/22); new base C (4/5); total (26/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01 | |||||||||||
TCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTGGTTGATCGCT > minE/2245506‑2245642 | tCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGCCaa < 1:2379956/71‑1 (MQ=255) cACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt > 1:48828/1‑71 (MQ=255) cACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt > 1:2496126/1‑71 (MQ=255) aCTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt > 1:350957/1‑70 (MQ=255) aCTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt < 1:2942731/70‑1 (MQ=255) aCTGGCATGTTTAACATGGTTTTCACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATc < 1:2384315/71‑1 (MQ=255) tGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCa < 1:418021/70‑1 (MQ=255) tGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTa < 1:938289/71‑1 (MQ=255) gTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTaa > 1:2457878/1‑71 (MQ=255) tttAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCt < 1:1542080/67‑1 (MQ=255) tAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAAc > 1:61373/1‑69 (MQ=255) aCATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGt > 1:1432585/1‑53 (MQ=255) aCATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGt > 1:3475554/1‑53 (MQ=255) gTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGAt < 1:2914386/71‑1 (MQ=255) ttACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAAcc > 1:481483/1‑71 (MQ=255) ttACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAAc < 1:3724701/70‑1 (MQ=255) aCATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCt > 1:827236/1‑70 (MQ=255) aCATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCt > 1:2220278/1‑70 (MQ=255) aTTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGa < 1:3001960/70‑1 (MQ=255) tttCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACg < 1:578601/71‑1 (MQ=255) tctcACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCGCGATAACCTGACgg < 1:1905517/70‑1 (MQ=255) cacACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGa < 1:1807284/68‑1 (MQ=255) aCTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTa < 1:2943362/45‑1 (MQ=255) aCTGAGCAGTTTTTGAATACAAACTTGCGGAGCCAATCATGCTTAACCAGCTCGATAACCTGAc > 1:2292706/1‑64 (MQ=255) aCTGAGCAGTTTTTGAATACAAACTTGCGGAGCCAATCATGCTTAACCAGCTCGATAACCTGACGGAg < 1:660035/68‑1 (MQ=255) aCTGAGCAGTATTTGAATACAAACTTGCGGAGTCAATCAAGCTAAACCAGCTCGATAACCTGACGGAgcgc > 1:1529093/1‑71 (MQ=255) aGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAAc > 1:2123245/1‑43 (MQ=255) aGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAAc > 1:1760548/1‑43 (MQ=255) aGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGt > 1:2879229/1‑69 (MQ=255) aGCAGTTTTTGAATACAAACTTGCGGAGCCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGt > 1:1051486/1‑69 (MQ=255) aGCAGTTTTTGAATACAAACTTGCGGAGCCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTc < 1:757182/70‑1 (MQ=255) tttttGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACg < 1:1938659/56‑1 (MQ=255) tttttGAATACAAACTTGCGGAGCCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAgg > 1:486991/1‑70 (MQ=255) ttttGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATa > 1:2161487/1‑47 (MQ=255) ttttGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCaga > 1:3087617/1‑67 (MQ=255) tttGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAg > 1:3184741/1‑71 (MQ=255) tGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAg < 1:1429551/69‑1 (MQ=255) aaTACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCa < 1:3385285/60‑1 (MQ=255) cAAACTTGCGGAGTCAATCATGCTTAACCAGCACGATAACCTGACgg > 1:3611803/1‑47 (MQ=255) aaaCTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAgc > 1:2355838/1‑49 (MQ=255) aaaCTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACt > 1:1519888/1‑71 (MQ=255) aaCTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTg > 1:3908832/1‑71 (MQ=255) aCTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTgg < 1:2154754/71‑1 (MQ=255) aCTTGCGGAGCCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTgg > 1:3776596/1‑71 (MQ=255) ttGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTGGtt > 1:2602607/1‑71 (MQ=255) ttGCGGAGCCAATCATGCTTAACCAGCTCGATAACCTGa < 1:2260759/39‑1 (MQ=255) cGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTGGTTGa < 1:1376199/70‑1 (MQ=255) gAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTGGTTGATc > 1:439155/1‑70 (MQ=255) gAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTGGTTGATc < 1:1654841/70‑1 (MQ=255) gAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTGGTTGATCg < 1:1152579/71‑1 (MQ=255) gAGCCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTGGTTGATCg < 1:2466398/71‑1 (MQ=255) aGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTGGTTGATCGc < 1:2684914/71‑1 (MQ=255) aGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTGGTTGATCGc > 1:2536967/1‑71 (MQ=255) aGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTGGTTGATCGc < 1:1362204/71‑1 (MQ=255) gTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTGGTTGATCGCt < 1:231531/71‑1 (MQ=255) tCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTGGTTGATCg < 1:1896448/68‑1 (MQ=255) | TCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTGGTTGATCGCT > minE/2245506‑2245642 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |