| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,264,627 | T→C | 12.5% | K6K (AAA→AAG) | nusG ← | transcription termination factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,264,627 | 0 | T | C | 12.5% | 185.2 / 16.2 | 72 | K6K (AAA→AAG) | nusG | transcription termination factor |
| Reads supporting (aligned to +/- strand): ref base T (37/26); new base C (5/4); total (42/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
GCTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATACCATC > minE/2264560‑2264696 | gCTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCtttt > 1:1377569/1‑69 (MQ=255) cTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCttt < 1:2040349/67‑1 (MQ=255) cTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTa < 1:2464972/70‑1 (MQ=255) aGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCtt < 1:1986871/70‑1 (MQ=255) aGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTc < 1:740811/71‑1 (MQ=255) cGACGCTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTc > 1:1782609/1‑69 (MQ=255) gACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGa > 1:246593/1‑71 (MQ=255) cGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACa > 1:1089922/1‑71 (MQ=255) tGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTa > 1:667016/1‑55 (MQ=255) tGCTACGAGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGa < 1:446268/58‑1 (MQ=255) cTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACAtct > 1:2522051/1‑69 (MQ=255) cgcgGCCTTCAAAACCGGAAAACGCCTGAACGACTTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAg < 1:3827349/69‑1 (MQ=255) gcgGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCtt > 1:3532733/1‑56 (MQ=255) gcgGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCtt > 1:336062/1‑56 (MQ=255) ggCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCt > 1:1268572/1‑71 (MQ=255) ggCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCt < 1:1403653/71‑1 (MQ=255) gCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTc < 1:2056295/71‑1 (MQ=255) cTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAg > 1:3310809/1‑71 (MQ=255) cTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAg > 1:949684/1‑71 (MQ=255) ttCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAg > 1:3314492/1‑70 (MQ=255) cAAAACCGGAAAACGCCTGAACGACGTACCAGCGcttctt < 1:1230274/40‑1 (MQ=255) cAAAACCGGAAAACGCCTGAACGACGTACCAGCGcttctt < 1:1536873/40‑1 (MQ=255) cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCttt > 1:1521743/1‑38 (MQ=255) cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:1222245/1‑70 (MQ=255) cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:2830570/1‑70 (MQ=255) cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:3365598/1‑70 (MQ=255) cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:3237980/1‑70 (MQ=255) aaaCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGa > 1:3463974/1‑42 (MQ=255) aaaCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGa > 1:1574735/1‑42 (MQ=255) aaaCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGa > 1:3286380/1‑42 (MQ=255) caCCGGAAAACGCCTGAACGACGTACCAGCGCttt < 1:2941321/34‑1 (MQ=255) aaCCGGAAAACGCCTGAACGACGTACCAGCGCttt < 1:3872057/35‑1 (MQ=255) aaCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGAc > 1:1901869/1‑50 (MQ=255) aCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACAt > 1:2143928/1‑51 (MQ=255) aCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGt > 1:2378916/1‑70 (MQ=255) aCCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAg > 1:612577/1‑69 (MQ=255) aCCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGt > 1:281783/1‑70 (MQ=255) aCCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGt > 1:2779133/1‑70 (MQ=255) aCCGGAAAACGCCTGAACGACGTACCAGCGATTTTTAGGAGCTTCAGACAt > 1:2662997/1‑51 (MQ=255) cGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGa > 1:2407527/1‑70 (MQ=255) ggAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATa < 1:682528/71‑1 (MQ=255) gAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATaa < 1:630997/71‑1 (MQ=255) aaaaCGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:1266886/1‑61 (MQ=255) aaaaCGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATaa < 1:789594/70‑1 (MQ=255) aaaCGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACAtctc > 1:2207786/1‑48 (MQ=255) cGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAAc > 1:1960002/1‑50 (MQ=255) gCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATaa < 1:144841/65‑1 (MQ=255) ccTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATaa < 1:468272/64‑1 (MQ=255) ccTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATAc < 1:3180449/71‑1 (MQ=255) ccTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATAc > 1:2343259/1‑71 (MQ=255) cTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATAcc < 1:2493191/71‑1 (MQ=255) cTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATAcc < 1:3416765/71‑1 (MQ=255) cTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCa < 1:3856333/51‑1 (MQ=255) cTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCa < 1:3612330/51‑1 (MQ=255) tGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACAt < 1:740231/38‑1 (MQ=255) tGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGa < 1:1649564/66‑1 (MQ=255) tGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATAc > 1:2755170/1‑69 (MQ=255) gAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTc > 1:769047/1‑48 (MQ=255) gAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTc > 1:2526206/1‑48 (MQ=255) gAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATa < 1:2466128/60‑1 (MQ=255) gAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATAc > 1:579203/1‑68 (MQ=255) gAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAg > 1:3836647/1‑71 (MQ=255) gTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAAcc < 1:1186225/71‑1 (MQ=255) tACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAAc > 1:18667/1‑69 (MQ=255) tACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCa < 1:403235/71‑1 (MQ=255) aCCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAg > 1:2087437/1‑46 (MQ=255) ccAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGa > 1:1817577/1‑55 (MQ=255) caaGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCa > 1:2583910/3‑44 (MQ=255) cAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGaa > 1:1324486/1‑71 (MQ=255) cAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGaa < 1:1627940/71‑1 (MQ=255) cAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGaa < 1:173228/71‑1 (MQ=255) aGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGAt < 1:3304165/47‑1 (MQ=255) aGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGAt < 1:1053311/47‑1 (MQ=255) aGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATaa < 1:149044/49‑1 (MQ=255) aGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGaa > 1:2136107/1‑70 (MQ=255) gcgcTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATa < 1:1328827/71‑1 (MQ=255) cgcTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATaa < 1:3884031/47‑1 (MQ=255) cTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGAt < 1:2942051/43‑1 (MQ=255) cTTTTTAGGAGCTTCAGACAACTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGa > 1:2788911/1‑59 (MQ=255) tttAGGCGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAgg > 1:509746/1‑53 (MQ=255) tttAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATACCATc > 1:3283468/1‑70 (MQ=255) | GCTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATACCATC > minE/2264560‑2264696 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |