| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,264,986 | T→C | 17.5% | E15G (GAA→GGA) | secE ← | preprotein translocase membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,264,986 | 0 | T | C | 17.5% | 116.0 / 17.7 | 54 | E15G (GAA→GGA) | secE | preprotein translocase membrane subunit |
| Reads supporting (aligned to +/- strand): ref base T (21/23); new base C (4/5); total (25/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01 | |||||||||||
GCGATAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGTTTTCTA > minE/2264919‑2265054 | gcgATAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTcc < 1:2233129/70‑1 (MQ=255)gcgATAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTc > 1:3756059/1‑69 (MQ=255) tAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGcc > 1:1456226/1‑71 (MQ=255) gATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCgcgc < 1:195942/71‑1 (MQ=255) aTAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGcc > 1:1959122/1‑71 (MQ=255) tAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAgg < 1:3849324/63‑1 (MQ=255) tAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGccc > 1:1392793/1‑71 (MQ=255) ttGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGccc < 1:3127290/68‑1 (MQ=255) ttGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCt < 1:6051/71‑1 (MQ=255) ttGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCt < 1:3332946/71‑1 (MQ=255) gCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTc > 1:271491/1‑71 (MQ=255) ccGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTc < 1:2411535/70‑1 (MQ=255) gACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCtt < 1:2936108/71‑1 (MQ=255) aCAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCtt > 1:1978140/1‑70 (MQ=255) cAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCtt > 1:2564499/1‑69 (MQ=255) tCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTcc < 1:1345801/47‑1 (MQ=255) tCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCtt < 1:1809272/66‑1 (MQ=255) caGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGcc < 1:2821995/45‑1 (MQ=255) caGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTa > 1:2466121/1‑70 (MQ=255) aGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTAtt < 1:2604789/71‑1 (MQ=255) ggAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTc > 1:2453430/1‑71 (MQ=255) aGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGccc > 1:3794598/1‑47 (MQ=255) aGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGccc > 1:64020/1‑47 (MQ=255) gCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCa < 1:2569683/71‑1 (MQ=255) aaTGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt < 1:281973/71‑1 (MQ=255) ccaccaCAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCa > 1:2410870/1‑65 (MQ=255) ccaccaCAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATa < 1:1282664/71‑1 (MQ=255) caccacAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATaa < 1:1231759/71‑1 (MQ=255) accacAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATaaa < 1:2355267/71‑1 (MQ=255) ccacAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATa < 1:2645782/68‑1 (MQ=255) acaACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCt < 1:1397814/48‑1 (MQ=255) acaACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaacc > 1:2725359/1‑70 (MQ=255) acaACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTc > 1:597199/1‑57 (MQ=255) caACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCAc < 1:2809823/60‑1 (MQ=255) caACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATaaa > 1:1384511/1‑67 (MQ=255) caACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaaccaa > 1:1952259/1‑71 (MQ=255) caACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTAGGTATTCGCACTCATAaaccaa > 1:356347/1‑71 (MQ=255) aaCGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTg < 1:3670524/42‑1 (MQ=255) aaCGACCCACTTCATCGCTTCCAGGCCGCGCCCGCCTCCTTGAGCTTCGGTATTCGCACTCATAaaccaac > 1:559705/1‑71 (MQ=255) cGACCCACTTCATCGCTACCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTAt < 1:624958/51‑1 (MQ=255) gACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTg < 1:1635080/71‑1 (MQ=255) gACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTg < 1:57384/71‑1 (MQ=255) cccACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTAtt > 1:343939/1‑49 (MQ=255) ccACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCtt < 1:574151/41‑1 (MQ=255) ccACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaaccaac > 1:1848967/1‑65 (MQ=255) cACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTAtt < 1:2989762/47‑1 (MQ=255) ttCATCGCTTCCAGGCCTCGCCCGCTTCCTTGAGCTTCGGTAtt < 1:3104158/44‑1 (MQ=255) tCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTc > 1:313015/1‑37 (MQ=255) cATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGtt > 1:2864868/1‑71 (MQ=255) cATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGtt > 1:1644528/1‑71 (MQ=255) aTCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGttt > 1:120098/1‑71 (MQ=255) tCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa < 1:3486205/48‑1 (MQ=255) tCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa < 1:2313962/48‑1 (MQ=255) cTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGTTTTCTa > 1:1403646/1‑71 (MQ=255) | GCGATAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGTTTTCTA > minE/2264919‑2265054 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |