| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,280,720 | G→A | 30.3% | R201* (CGA→TGA) | fabR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,280,720 | 0 | G | A | 30.3% | 76.2 / 40.2 | 56 | R201* (CGA→TGA) | fabR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (16/23); new base A (12/5); total (28/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.97e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
TTTTCTCTTGTTCACGGCGATACCAGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCTCGGCACCCGCACTGAAGACAA > minE/2280651‑2280785 | ttttCTCTTGTTCACGGCGATACCAGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGc > 1:2444611/1‑71 (MQ=255) ttGTTCACGGCGATACCAGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGCtctt < 1:2846813/68‑1 (MQ=255) ttGTTCACAGCGATACCAGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCActc < 1:2666389/66‑1 (MQ=255) gTTCACGGCGATACCAGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGCTCTTCTaa < 1:1220403/70‑1 (MQ=255) aCGGCGATACCAGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGcc < 1:1189981/71‑1 (MQ=255) cGGCGATACCAGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCg < 1:1355680/71‑1 (MQ=255) gCGATACCAGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCACTCTTCTAATTGCCGAc < 1:404785/71‑1 (MQ=255) taTACCAGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGAAGTACCAGTCGCTCTTCTAATTGCCg > 1:2736721/2‑67 (MQ=255) gATACCAGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGAc > 1:3725502/1‑69 (MQ=255) gATACCAGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGAc > 1:3720841/1‑69 (MQ=255) tACCAGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCg > 1:1352363/1‑50 (MQ=255) tACCAGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACgttg < 1:2548491/71‑1 (MQ=255) ccAGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACgttgtt < 1:2707388/71‑1 (MQ=255) ccAGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACgttgt < 1:1222453/70‑1 (MQ=255) cAGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGctct < 1:1086789/51‑1 (MQ=255) aGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTc > 1:488729/1‑70 (MQ=255) aGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTCg < 1:206130/71‑1 (MQ=255) gTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCACTCTTCTAATTGCCGACGTTGTTCGa > 1:2491908/1‑71 (MQ=255) gTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCACTCTTCTAATTGCCGACGTTGTTCGa > 1:3044293/1‑71 (MQ=255) gTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCACTCTTCTAATTGCCGACGTTGTTCGa > 1:2704167/1‑71 (MQ=255) taataaGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGCTCTTCTaa < 1:3024591/53‑1 (MQ=255) taataaGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACgttgt < 1:950147/66‑1 (MQ=255) taataaGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCACTCTTCTAATTGCCGACGTTGTTCGAc > 1:3231283/1‑71 (MQ=255) ataaGCCCCTTTCGAAATAATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTc > 1:3129101/1‑66 (MQ=255) ataaGCCCCTTTCGAAATAATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTc > 1:3133500/1‑66 (MQ=255) aGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTCGACGCCGa > 1:455231/1‑71 (MQ=255) cccTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGctc > 1:2172672/1‑39 (MQ=255) cccTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAAtt < 1:1465118/47‑1 (MQ=255) cccTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTCGACGCCGACGt < 1:660469/71‑1 (MQ=255) gAAATCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGc > 1:196289/1‑71 (MQ=255) aaaTCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTCGACGCCGACGT‑CAACGCCt < 1:2758353/71‑1 (MQ=255) aaTCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTCg > 1:3824618/1‑52 (MQ=255) aaTCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCt < 1:2859376/71‑1 (MQ=255) aaTCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCt < 1:3838214/71‑1 (MQ=255) aTCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCt < 1:3745131/70‑1 (MQ=255) aTCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCt < 1:568202/70‑1 (MQ=255) aTCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCTc > 1:638567/1‑71 (MQ=255) aTCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCTc > 1:3826911/1‑71 (MQ=255) aTCATTCGCAGTTGCAGTACCAGTCGCTATTCTAATTGCCGACGTTGTTCGACGCCg > 1:3131689/1‑57 (MQ=255) aTCATTCGCAGTTGCAGTACCAGTCACTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCTc > 1:3864641/1‑71 (MQ=255) aTCATTCGCAGTTGCAGTACCAGTCACTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCTc > 1:1265024/1‑71 (MQ=255) tCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCTCg < 1:368088/71‑1 (MQ=255) tCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTGGACGTCGGCg > 1:69428/1‑55 (MQ=255) aGTTGCAGTACCAGTCACTCTTCTAATTGCCGACGTTGTTCGa > 1:776582/1‑43 (MQ=255) gTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCTCGGCACCCGCa < 1:1080157/71‑1 (MQ=255) gTTGCAGTACCAGGCGCTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCTCGGCACCCGCa < 1:3453145/71‑1 (MQ=255) ttGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCTCGGCACCCGCAc > 1:1303221/1‑71 (MQ=255) cAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCTCGGCACCCGCACTg < 1:3805537/70‑1 (MQ=255) cAGTACCAGTCACTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCTCGGCACCCGCACTg < 1:3412211/70‑1 (MQ=255) aGTACCAGTCACTCTTCTAATTGCCGACGTTGTTCg > 1:995572/1‑36 (MQ=255) aGTACCAGTCACTCTTCTAATTGCCGACGTTGTTCg > 1:3864287/1‑36 (MQ=255) aGTACCAGTCACTCTTCTAATTGCCGACGTTGTTCg > 1:3110414/1‑36 (MQ=255) ccAGTCACTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCTCGGCACCCGCACTGAAGAc > 1:136029/1‑70 (MQ=255) cAGTCGCTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAAc < 1:3414459/46‑1 (MQ=255) cAGTCACTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCTc < 1:2431194/51‑1 (MQ=255) cAGTCACTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCTCGGCACCCGCACTGAAGACaa > 1:372488/1‑71 (MQ=255) cAGTCACTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCTCGGCACCCGCACTGAAGACaa < 1:1036203/71‑1 (MQ=255) | TTTTCTCTTGTTCACGGCGATACCAGTAATAAGCCCCTTTCGAAATCATTCGCAGTTGCAGTACCAGTCGCTCTTCTAATTGCCGACGTTGTTCGACGCCGACGTCCAACGCCTCGGCACCCGCACTGAAGACAA > minE/2280651‑2280785 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |