| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,292,617 | A→G | 51.7% | intergenic (+168/‑253) | fsaB → / → gldA | fructose‑6‑phosphate aldolase 2/glycerol dehydrogenase, NAD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,292,617 | 0 | A | G | 51.7% | 4.6 / 76.0 | 58 | intergenic (+168/‑253) | fsaB/gldA | fructose‑6‑phosphate aldolase 2/glycerol dehydrogenase, NAD |
| Reads supporting (aligned to +/- strand): ref base A (13/15); new base G (14/16); total (27/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATTAGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGC > minE/2292549‑2292687 | tattaGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGAc > 1:1806757/1‑71 (MQ=255)tattaGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGAc > 1:1717600/1‑71 (MQ=255) ttaGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACgg < 1:2191904/71‑1 (MQ=255) taGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATACCCAGGGCGGAAACgg > 1:3649662/1‑70 (MQ=255) gCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCAt > 1:2736979/1‑71 (MQ=255) gCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCAt > 1:1264819/1‑71 (MQ=255) gCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCAt > 1:3872384/1‑71 (MQ=255) cGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTc > 1:1099856/1‑71 (MQ=255) cGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTc > 1:2359933/1‑71 (MQ=255) gCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCg < 1:2436150/71‑1 (MQ=255) gCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCg > 1:1162525/1‑71 (MQ=255) cTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTAc > 1:1889125/1‑71 (MQ=255) aGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGtt > 1:1522448/1‑70 (MQ=255) cAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAg < 1:2490910/71‑1 (MQ=255) cAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAg > 1:2686493/1‑71 (MQ=255) aaaTACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGc < 1:3519674/70‑1 (MQ=255) cGTTGCTCCGTATGTTAACCGCGTAGATGACCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCaaa > 1:804589/1‑71 (MQ=255) gTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCaaa > 1:1622517/1‑70 (MQ=255) gCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCt > 1:3718908/1‑71 (MQ=255) cTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCAtt < 1:3338192/44‑1 (MQ=255) cTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCAtt < 1:2547203/44‑1 (MQ=255) cTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCAtt < 1:3877418/44‑1 (MQ=255) cTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCt < 1:1339555/70‑1 (MQ=255) cTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCa < 1:416351/64‑1 (MQ=255) tCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAgg < 1:131046/56‑1 (MQ=255) tCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAg < 1:1059568/58‑1 (MQ=255) cGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGt > 1:681528/1‑69 (MQ=255) cGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGc > 1:3431525/1‑60 (MQ=255) cGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGt < 1:3690559/69‑1 (MQ=255) gTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGtt < 1:1984820/69‑1 (MQ=255) aTGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTc < 1:1725823/48‑1 (MQ=255) aTGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGa > 1:3084988/1‑70 (MQ=255) aTGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGa > 1:922830/1‑70 (MQ=255) tGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaa < 1:94970/70‑1 (MQ=255) tGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaa < 1:3300024/70‑1 (MQ=255) tGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaa < 1:1368391/70‑1 (MQ=255) ttAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAAt < 1:3882769/70‑1 (MQ=255) tAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCg > 1:1487228/1‑36 (MQ=255) aaCCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGc < 1:1163760/70‑1 (MQ=255) aCCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCAc < 1:2731668/71‑1 (MQ=255) aCCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCAc > 1:1969961/1‑71 (MQ=255) aCCGCCTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACCCTGTTAGAAATGCAc < 1:501305/71‑1 (MQ=255) cgTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGTAGCTGCAAACGCTGTTAGAAATGCACgcgc < 1:178876/71‑1 (MQ=255) gTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCAcgcg > 1:2832897/1‑69 (MQ=255) tAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCt > 1:2700980/1‑43 (MQ=255) tAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCa < 1:962730/37‑1 (MQ=255) tAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCa < 1:3085428/37‑1 (MQ=255) tGCCCAGGGCGGAAACGTCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGaaa < 1:499470/71‑1 (MQ=255) gCCCAGGGCGGAGACGGCATTCGTATGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAg > 1:383819/1‑71 (MQ=255) cccAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAg < 1:2537589/70‑1 (MQ=255) cccAGGGCGGAGACGACATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAg < 1:1091303/70‑1 (MQ=255) ccAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCa > 1:2009602/1‑71 (MQ=255) aGGGCGGAGACGACATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATg > 1:1373837/1‑71 (MQ=255) aGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATg > 1:3626611/1‑71 (MQ=255) ggCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGt < 1:705746/71‑1 (MQ=255) ggCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGt < 1:3157331/71‑1 (MQ=255) gCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTg < 1:3375811/71‑1 (MQ=255) cGGAAAGGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATTGTGc < 1:1687632/71‑1 (MQ=255) aaaCGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTgg > 1:2364351/1‑71 (MQ=255) aaCGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGc < 1:2197832/71‑1 (MQ=255) | TATTAGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGC > minE/2292549‑2292687 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |