| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,294,050 | A→C | 26.0% | intergenic (+77/‑198) | gldA → / → yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,294,050 | 0 | A | C | 26.0% | 103.4 / 53.2 | 73 | intergenic (+77/‑198) | gldA/yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (30/24); new base C (14/5); total (44/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGG > minE/2293983‑2294115 | aaCTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAAtc > 1:995316/1‑71 (MQ=255)aaCTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAAtc > 1:3502041/1‑71 (MQ=255) aCTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAAtct < 1:3113736/71‑1 (MQ=255) cGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCa < 1:379824/71‑1 (MQ=255) ggCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCaa > 1:3294144/1‑71 (MQ=255) gCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCaa > 1:1973101/1‑70 (MQ=255) gTCCAGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAatata > 1:1330705/1‑70 (MQ=255) ccGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATAtt > 1:3253906/1‑70 (MQ=255) ggAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAt > 1:1401993/1‑71 (MQ=255) ggAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAt > 1:2611737/1‑71 (MQ=255) ggAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAt > 1:2128710/1‑71 (MQ=255) aGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAttt < 1:3367968/71‑1 (MQ=255) gTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTg < 1:1289934/71‑1 (MQ=255) tttGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAttt < 1:1598888/69‑1 (MQ=255) ttGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGc < 1:1254907/70‑1 (MQ=255) ttGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCg > 1:3073908/1‑71 (MQ=255) tGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAAtctc < 1:2943384/50‑1 (MQ=255) tGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGc > 1:47669/1‑69 (MQ=255) accGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGaa > 1:1897338/3‑62 (MQ=14) accGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGc < 1:3647828/69‑1 (MQ=14) aaCGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGc > 1:657255/1‑71 (MQ=255) ccGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCt > 1:1373522/2‑71 (MQ=255) aCGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGc > 1:3782285/1‑66 (MQ=255) aCGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCt < 1:352729/71‑1 (MQ=255) aCGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCt < 1:2571491/71‑1 (MQ=255) cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCGTACGCCATCTCTGCAATATATTGAAtt > 1:1733740/1‑62 (MQ=14) gCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATAtt < 1:2066470/56‑1 (MQ=255) aaaaATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:62027/1‑71 (MQ=255) aaaaATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:3496625/1‑71 (MQ=255) aaaaTGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGt < 1:799777/71‑1 (MQ=255) aaaTTGCCTTATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:2628688/1‑70 (MQ=255) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:3815840/1‑70 (MQ=255) aaatGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:960635/4‑69 (MQ=255) aaTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCttt > 1:1960770/1‑67 (MQ=255) aaTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:311150/1‑69 (MQ=255) aaTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:3048736/1‑69 (MQ=255) gCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTa > 1:2573273/1‑67 (MQ=255) gCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTa > 1:3302748/1‑67 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCtt < 1:2686658/61‑1 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGt < 1:465331/65‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAAt < 1:1818995/47‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGAt > 1:1089080/1‑70 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGAt > 1:2431973/1‑70 (MQ=255) gcTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg < 1:1548148/56‑1 (MQ=255) tACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAg > 1:1461516/1‑67 (MQ=255) tACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGATTTTGTAGGCAGGATAAg > 1:2979552/1‑67 (MQ=14) cGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTc < 1:1533679/71‑1 (MQ=255) cGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt > 1:1554278/1‑70 (MQ=255) cGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt > 1:293116/1‑70 (MQ=255) cGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGt < 1:3173641/69‑1 (MQ=255) ttATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCg < 1:321679/65‑1 (MQ=255) ttATCAGGCCTAAGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACt > 1:2043816/1‑71 (MQ=255) aTCAGGCCTACGCAATCTCTGCAATATATTGAAtt < 1:1490344/35‑1 (MQ=255) aTCAGGCCTACGCAATCTCTGCAATATATTGAAtt < 1:2318912/35‑1 (MQ=255) aTCAGGCCTACGCAATCTCTGCAATATATTGAATTTg < 1:1669030/37‑1 (MQ=255) aTCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGc > 1:529753/1‑42 (MQ=255) tCAGGCCTACGCAATCTCTGCAATATATTGAATTTGc > 1:3107459/1‑37 (MQ=255) tCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTcc < 1:1103155/70‑1 (MQ=255) tCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCg < 1:1062469/71‑1 (MQ=255) aGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGc > 1:414829/1‑59 (MQ=17) aGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCa > 1:2111750/1‑71 (MQ=14) aGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCa > 1:2116405/1‑71 (MQ=14) aGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCAc > 1:1103871/1‑65 (MQ=255) aGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCa > 1:972255/1‑71 (MQ=255) ggCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCAt < 1:3878604/71‑1 (MQ=255) gCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATc < 1:2697775/71‑1 (MQ=255) ccTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATcc > 1:560551/1‑71 (MQ=11) tACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTCGTa < 1:960924/41‑1 (MQ=255) aCGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCgg > 1:3306699/1‑70 (MQ=255) cGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGt > 1:3496042/1‑38 (MQ=255) cGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCgg < 1:3844082/69‑1 (MQ=255) cAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATa < 1:3845639/46‑1 (MQ=255) cAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATcc > 1:2099575/1‑65 (MQ=255) aaTCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATa < 1:769919/45‑1 (MQ=255) aaTCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATa < 1:1773593/45‑1 (MQ=255) | AACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGG > minE/2293983‑2294115 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |