| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,294,089 | C→A | 19.2% | intergenic (+116/‑159) | gldA → / → yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,294,089 | 0 | C | A | 19.2% | 139.4 / 14.7 | 52 | intergenic (+116/‑159) | gldA/yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (18/24); new base A (8/2); total (26/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.51e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
ATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAATT > minE/2294024‑2294159 | aTGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGAt > 1:2431973/1‑70 (MQ=255)aTGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGAt > 1:1089080/1‑70 (MQ=255) tACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAg > 1:1461516/1‑67 (MQ=255) tACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGATTTTGTAGGCAGGATAAg > 1:2979552/1‑67 (MQ=14) cGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTc < 1:1533679/71‑1 (MQ=255) cGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt > 1:293116/1‑70 (MQ=255) cGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt > 1:1554278/1‑70 (MQ=255) cGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGt < 1:3173641/69‑1 (MQ=255) ttATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCg < 1:321679/65‑1 (MQ=255) ttATCAGGCCTAAGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACt > 1:2043816/1‑71 (MQ=255) tCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTcc < 1:1103155/70‑1 (MQ=255) tCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCg < 1:1062469/71‑1 (MQ=255) aGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGc > 1:414829/1‑59 (MQ=17) aGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCa > 1:2116405/1‑71 (MQ=14) aGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCa > 1:2111750/1‑71 (MQ=14) aGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCAc > 1:1103871/1‑65 (MQ=255) aGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCa > 1:972255/1‑71 (MQ=255) ggCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCAt < 1:3878604/71‑1 (MQ=255) gCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATc < 1:2697775/71‑1 (MQ=255) ccTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATcc > 1:560551/1‑71 (MQ=11) aCGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCgg > 1:3306699/1‑70 (MQ=255) cGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCgg < 1:3844082/69‑1 (MQ=255) cAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATa < 1:3845639/46‑1 (MQ=255) cAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATcc > 1:2099575/1‑65 (MQ=255) aaTCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATa < 1:769919/45‑1 (MQ=255) aaTCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATa < 1:1773593/45‑1 (MQ=255) aTCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCActct < 1:818982/71‑1 (MQ=255) aTCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCActct < 1:976881/71‑1 (MQ=255) tGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt < 1:1529479/38‑1 (MQ=40) aaTATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATcc > 1:1436048/1‑55 (MQ=255) atatatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGc > 1:1873816/1‑39 (MQ=40) tatatTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCg < 1:3311391/39‑1 (MQ=255) tatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAAt < 1:2116238/70‑1 (MQ=255) tatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAAt < 1:1429239/70‑1 (MQ=255) tGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTg > 1:1637148/1‑70 (MQ=255) gAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGa < 1:2815262/70‑1 (MQ=255) gAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGa < 1:1566549/70‑1 (MQ=255) aaTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGaa < 1:1661105/70‑1 (MQ=255) tttGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGa < 1:3630816/67‑1 (MQ=255) tGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTcc > 1:2398074/1‑71 (MQ=255) tGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTc < 1:957084/70‑1 (MQ=255) gCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTcc > 1:994397/1‑70 (MQ=255) gCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTcc > 1:2090238/1‑70 (MQ=255) cGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTccc < 1:2630861/70‑1 (MQ=255) gCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACgt > 1:649805/1‑71 (MQ=255) ttttGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGa > 1:2168527/1‑58 (MQ=255) ttGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTg > 1:456866/1‑55 (MQ=255) ttGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGaaa > 1:1024017/1‑58 (MQ=255) tGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACCCCCACgttgtt < 1:211526/70‑1 (MQ=255) gTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACgttgtt > 1:1767428/1‑69 (MQ=255) gTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTc < 1:889807/70‑1 (MQ=255) gCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGaa < 1:853623/71‑1 (MQ=255) cGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTg < 1:2465430/47‑1 (MQ=255) cGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAAtt < 1:3101121/71‑1 (MQ=255) | ATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAATT > minE/2294024‑2294159 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |